Detailed information NTG0133345

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PN96_RS15040
• Genome accession: NZ_CP009977
• Coordinates: 3221823..3222839 (+)
• Length: 338 a.a.
• NCBI ID: WP_020334617.1
• Uniprot ID: A0AAN0Y4M4
• Organism: Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3216823..3227839

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PN96_RS15025 (PN96_14815)	-	3217253..3217981 (-)	729	WP_020334619.1	redoxin family protein	-
PN96_RS15030 (PN96_14820)	oxyR	3218140..3219048 (+)	909	WP_014233154.1	DNA-binding transcriptional regulator OxyR	-
PN96_RS15035 (PN96_14825)	-	3219112..3221679 (-)	2568	WP_020334618.1	PBP1A family penicillin-binding protein	-
PN96_RS15040 (PN96_14830)	pilM	3221823..3222839 (+)	1017	WP_020334617.1	type IV pilus assembly protein PilM	Machinery gene
PN96_RS15045 (PN96_14835)	pilN	3222823..3223398 (+)	576	WP_024372660.1	PilN domain-containing protein	Machinery gene
PN96_RS15050 (PN96_14840)	pilO	3223391..3223984 (+)	594	WP_020334615.1	type 4a pilus biogenesis protein PilO	Machinery gene
PN96_RS15055 (PN96_14845)	pilP	3223974..3224489 (+)	516	WP_020334614.1	pilus assembly protein PilP	Machinery gene
PN96_RS15060 (PN96_14850)	pilQ	3224521..3226260 (+)	1740	WP_024372659.1	type IV pilus secretin PilQ family protein	Machinery gene
PN96_RS15065 (PN96_14855)	aroK	3226449..3226967 (+)	519	WP_014233147.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37587.78 Da
• Isoelectric Point: 7.2098

>NTDB_id=133345 PN96_RS15040 WP_020334617.1 3221823..3222839(+) (pilM) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
MDKRIVTGIDIGHNSLKAVVLKPNGEQFALLGYKEILLKDGIVAENNTINHQEIVNTLKQVKKDLPFGASRVAISVPDNA
VISKKLHIEQDLEENEIEFAVVQAFSHQSPFPVDELSLDFVRLNHEGGLRGPVSYQVFATRKDVVESRVEAIRKSGFKPV
LVEVHSQSLGQIWKLATERFPDKSQYGLLDVGSVSSSFTMFTEQNELFHKEFACGTGSIQCSVQESPLSGEKQAKTSQFN
RQMVDRVKRQIQLYTSINGSQNIKGLWLSGEGSTTPMLAEELSHQLAVECELLNPLGLFEMNVAKRKRRAVEWQNFSTAA
GLAVRGIQWLGGHRATSN

Nucleotide

• Length: 1017 bp

>NTDB_id=133345 PN96_RS15040 WP_020334617.1 3221823..3222839(+) (pilM) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
ATGGATAAGCGAATCGTTACAGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTCGTACTTAAACCCAATGGCGAGCA
ATTCGCCTTGTTGGGTTACAAAGAAATATTACTTAAGGATGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTTAAACAGGTGAAAAAAGACCTGCCATTTGGTGCAAGCAGAGTGGCAATTTCAGTGCCTGATAATGCG
GTTATCAGCAAAAAACTGCACATCGAACAAGACCTTGAAGAGAACGAAATAGAGTTCGCGGTCGTGCAGGCATTTTCTCA
TCAATCTCCTTTCCCCGTAGATGAGTTGAGTCTGGACTTTGTCCGTCTTAATCATGAAGGAGGCCTGCGAGGGCCAGTTA
GCTACCAAGTTTTTGCAACACGTAAAGATGTCGTGGAGAGTCGGGTTGAAGCAATACGTAAATCCGGTTTTAAACCTGTC
CTCGTCGAGGTCCATTCACAAAGCCTCGGGCAGATATGGAAACTGGCCACAGAACGTTTTCCAGATAAAAGTCAGTACGG
TTTGCTTGATGTTGGTTCTGTGTCGAGCTCTTTCACGATGTTCACCGAGCAAAACGAGCTATTTCATAAAGAATTTGCCT
GTGGAACAGGTTCCATTCAGTGCTCTGTGCAGGAAAGTCCTCTGTCCGGAGAAAAGCAAGCCAAAACGAGTCAATTTAAC
CGTCAGATGGTCGATCGTGTCAAACGTCAGATACAGCTTTATACGTCGATTAATGGGTCACAAAACATCAAAGGTCTTTG
GTTATCCGGTGAAGGGTCAACAACGCCAATGTTGGCTGAGGAGTTATCTCATCAATTGGCGGTCGAGTGCGAGTTACTGA
ATCCTTTAGGCCTATTTGAAATGAACGTCGCTAAACGTAAGCGTCGAGCGGTGGAATGGCAGAATTTTTCAACCGCCGCT
GGCTTGGCAGTACGAGGTATCCAATGGTTGGGAGGTCATCGTGCTACATCGAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-326)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio campbellii strain DS40M4	74.556	100	0.746
pilM	Vibrio cholerae strain A1552	48.521	100	0.485