Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   PN96_RS15045 Genome accession   NZ_CP009977
Coordinates   3222823..3223398 (+) Length   191 a.a.
NCBI ID   WP_024372660.1    Uniprot ID   -
Organism   Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3217823..3228398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PN96_RS15030 (PN96_14820) oxyR 3218140..3219048 (+) 909 WP_014233154.1 DNA-binding transcriptional regulator OxyR -
  PN96_RS15035 (PN96_14825) - 3219112..3221679 (-) 2568 WP_020334618.1 PBP1A family penicillin-binding protein -
  PN96_RS15040 (PN96_14830) pilM 3221823..3222839 (+) 1017 WP_020334617.1 type IV pilus assembly protein PilM Machinery gene
  PN96_RS15045 (PN96_14835) pilN 3222823..3223398 (+) 576 WP_024372660.1 PilN domain-containing protein Machinery gene
  PN96_RS15050 (PN96_14840) pilO 3223391..3223984 (+) 594 WP_020334615.1 type 4a pilus biogenesis protein PilO Machinery gene
  PN96_RS15055 (PN96_14845) pilP 3223974..3224489 (+) 516 WP_020334614.1 pilus assembly protein PilP Machinery gene
  PN96_RS15060 (PN96_14850) pilQ 3224521..3226260 (+) 1740 WP_024372659.1 type IV pilus secretin PilQ family protein Machinery gene
  PN96_RS15065 (PN96_14855) aroK 3226449..3226967 (+) 519 WP_014233147.1 shikimate kinase AroK -
  PN96_RS15070 (PN96_14860) aroB 3226999..3228099 (+) 1101 WP_020334612.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22349.65 Da        Isoelectric Point: 5.8969

>NTDB_id=133346 PN96_RS15045 WP_024372660.1 3222823..3223398(+) (pilN) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
MLHRINLLPWRENQREEHRRRFVGMFILGLFCAVGIQWGIGQFYEIQQDQQQDRLDYLTHYITELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEIAGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKERFGKEFQTFKVSFIFKAVEQEEGGQHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=133346 PN96_RS15045 WP_024372660.1 3222823..3223398(+) (pilN) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
GTGCTACATCGAATTAACTTATTGCCCTGGAGGGAGAACCAAAGGGAAGAACATCGCCGTCGTTTTGTTGGCATGTTCAT
TCTGGGCTTGTTTTGTGCTGTTGGTATTCAATGGGGGATTGGGCAGTTTTATGAGATCCAACAAGATCAGCAGCAAGACC
GACTCGACTATCTTACTCACTATATTACGGAACTTGATCAACGTATTGAAGCAATGAAAATTGCCGAGCAAGAGCACAGC
AAAATATTAGAGCGTTTGAAGGTGGTCGAAGGCTTGCAAAATGGTCGCAACAAAACCACAGAGTTCATGAACCTGATGCC
TGCTGTTATTCCTGAAGGCGTTTACGTTGATAAGATCAAAATGAATGATCTGGAAATCGAAATCGCGGGGATTTCTGATA
GCACACCACGTCTGGCAACCATGCTCGATAACATGGAGCGTTCGGCAAAACTATTGGACGTTGAAATGCACTCTATTGTC
CATGGCAAAGAACGTTTCGGTAAAGAATTTCAGACCTTCAAAGTGTCTTTCATATTTAAAGCGGTAGAGCAGGAAGAGGG
AGGTCAGCATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

81.152

100

0.812
  pilN Vibrio cholerae strain A1552

59.896

100

0.602

Multiple sequence alignment