Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NJ69_RS20175 Genome accession   NZ_CP009747
Coordinates   4604843..4606069 (+) Length   408 a.a.
NCBI ID   WP_116887364.1    Uniprot ID   A0AAI8K8K8
Organism   Pseudomonas parafulva strain CRS01-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4599843..4611069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NJ69_RS20160 (NJ69_20150) pilM 4602417..4603325 (+) 909 WP_167335977.1 pilus assembly protein PilM -
  NJ69_RS20165 (NJ69_20155) - 4603322..4603855 (+) 534 WP_039582579.1 PilN domain-containing protein -
  NJ69_RS20170 (NJ69_20160) - 4603870..4604877 (+) 1008 WP_162889484.1 pilus assembly protein PilP -
  NJ69_RS20175 (NJ69_20165) pilQ 4604843..4606069 (+) 1227 WP_116887364.1 secretin N-terminal domain-containing protein Machinery gene
  NJ69_RS20180 (NJ69_20170) aroK 4606074..4606592 (+) 519 WP_039582580.1 shikimate kinase AroK -
  NJ69_RS20185 (NJ69_20175) aroB 4606688..4607803 (+) 1116 WP_039582581.1 3-dehydroquinate synthase -
  NJ69_RS20190 (NJ69_20180) - 4607800..4609425 (+) 1626 WP_029614299.1 AAA family ATPase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 43752.91 Da        Isoelectric Point: 5.3254

>NTDB_id=131837 NJ69_RS20175 WP_116887364.1 4604843..4606069(+) (pilQ) [Pseudomonas parafulva strain CRS01-1]
MQALATTAYKGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDTVQGNVTLRLQDVPWDQALDLVLRSRGLARREQGNVLL
IAPAAELAEQTRETQADRLLEARSQPLQRALLPVHHADASALAPLLLASLALDDPADARATLSVDPRTNTLVAVQSAGRL
AELRQLIAQLDVPVRQVMIEARIVEANVDYEKVLGVRWGAKHGGSPGRSGSELFVDLGASPATSGIGLGLVGSNALLDLQ
LSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQQSSKSGATSVSFREASLSLEVTPQITPDGKVIMAVRVTKDEPD
YANALNDVPPIRKNEVNAKVRVADGQTIVIGGVYSTSNSNVVDKVPFLGDLPYVGRLFRRDALQEKKSELLVFLTPRIMN
DQAIAVSR

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=131837 NJ69_RS20175 WP_116887364.1 4604843..4606069(+) (pilQ) [Pseudomonas parafulva strain CRS01-1]
ATGCAGGCACTGGCGACGACGGCCTACAAAGGTGAGCCGCTGTCGCTGAACTTCCAGGATGTGGAGGTGCGAGCGGTGCT
GCAGGTGCTGGCCGACTATGCAGGGGTCAATCTGGTGGCCAGCGACACGGTCCAGGGCAACGTCACGCTGCGCTTGCAGG
ACGTACCCTGGGACCAGGCGCTGGACCTGGTGCTGCGCAGTCGCGGATTGGCCAGGCGCGAGCAGGGTAATGTACTGCTG
ATCGCCCCTGCCGCCGAGCTTGCCGAGCAAACGCGCGAGACGCAGGCTGACCGCCTGCTGGAGGCGCGGTCGCAGCCGCT
GCAGCGCGCACTGCTGCCCGTTCACCATGCCGATGCGTCCGCGCTGGCACCGCTGTTGCTGGCCAGCCTGGCCCTCGATG
ACCCGGCGGATGCGCGCGCCACGCTGAGCGTGGATCCACGCACCAACACCCTCGTCGCCGTCCAGTCCGCCGGGCGGCTG
GCCGAACTGCGGCAGTTGATCGCGCAGTTGGACGTGCCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAA
CGTCGACTACGAGAAGGTGCTTGGCGTACGCTGGGGCGCCAAACATGGCGGTTCGCCGGGACGCTCGGGCAGCGAACTGT
TCGTCGATCTCGGCGCCTCGCCCGCCACGTCCGGCATCGGTCTAGGCTTGGTGGGCAGCAATGCGCTGCTCGACTTGCAG
CTCAGCGCCATGGAAAAGAGCGGGAATGGCGAGATCATCTCGCAGCCCAAGGTGGTCACGGCCGACAAGGAAACCGCACG
CATCCTCAAGGGCACCGAGGTGCCGTACCAGCAGTCGAGCAAGAGTGGCGCCACCTCGGTGTCGTTCCGCGAGGCCTCGC
TTTCATTGGAGGTCACCCCGCAAATCACCCCTGACGGCAAAGTCATCATGGCGGTGCGCGTGACCAAGGACGAGCCCGAC
TACGCCAATGCCCTCAACGACGTGCCGCCGATTCGCAAGAACGAGGTCAACGCCAAGGTACGCGTGGCCGACGGTCAGAC
CATTGTGATTGGTGGCGTGTATTCGACGTCGAACAGCAATGTGGTCGACAAGGTGCCATTTCTTGGCGATCTGCCGTATG
TTGGACGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAAT
GACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(243-399)

Bacterial type II/III secretion system short domain (Secretin_N)

(111-176)

Secretin and TonB N terminus short domain (STN)

(37-83)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.916

100

0.64
  pilQ Acinetobacter baumannii strain A118

41.432

100

0.468
  pilQ Acinetobacter baumannii D1279779

41.432

100

0.468
  comQ Acinetobacter baylyi ADP1

42.444

100

0.468
  pilQ Legionella pneumophila strain ERS1305867

41.549

100

0.434
  pilQ Legionella pneumophila strain Lp02

41.549

100

0.434
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.766

100

0.429
  pilQ Vibrio cholerae strain A1552

41.766

100

0.429
  pilQ Vibrio campbellii strain DS40M4

40.566

100

0.422
  pilQ Neisseria gonorrhoeae strain FA1090

37.269

100

0.395
  pilQ Neisseria meningitidis M1080

37.355

100

0.395
  pilQ Neisseria meningitidis MC58

37.355

100

0.395

Multiple sequence alignment