Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   BWEI_RS13470 Genome accession   NZ_CP009746
Coordinates   2692642..2692980 (-) Length   112 a.a.
NCBI ID   WP_002012564.1    Uniprot ID   J8I4L2
Organism   Bacillus mycoides strain WSBC10204     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2692642..2710863 2692642..2692980 within 0


Gene organization within MGE regions


Location: 2692642..2710863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWEI_RS13470 (bwei_2854) ssbA 2692642..2692980 (-) 339 WP_002012564.1 single-stranded DNA-binding protein Machinery gene
  BWEI_RS13475 (bwei_2855) - 2693142..2693396 (-) 255 WP_002012563.1 DUF4318 domain-containing protein -
  BWEI_RS13480 (bwei_2856) - 2693491..2694222 (-) 732 WP_002127140.1 Bax inhibitor-1/YccA family protein -
  BWEI_RS13485 (bwei_2857) - 2694298..2696193 (-) 1896 WP_002127139.1 ABC-F family ATP-binding cassette domain-containing protein -
  BWEI_RS13490 (bwei_2858) - 2696190..2696837 (-) 648 WP_002031647.1 HD domain-containing protein -
  BWEI_RS13495 (bwei_2859) - 2696947..2697642 (+) 696 WP_002031649.1 hypothetical protein -
  BWEI_RS30635 (bwei_2860) - 2698235..2698387 (+) 153 WP_002031650.1 hypothetical protein -
  BWEI_RS28585 - 2698563..2698705 (+) 143 Protein_2792 DUF3956 family protein -
  BWEI_RS13505 (bwei_2861) - 2699173..2699688 (-) 516 WP_000512143.1 DUF3231 family protein -
  BWEI_RS13510 (bwei_2862) - 2700034..2700339 (+) 306 WP_002031652.1 WGxxGxxG family protein -
  BWEI_RS13515 (bwei_2863) - 2700709..2701251 (-) 543 WP_002127134.1 site-specific integrase -
  BWEI_RS13520 (bwei_2864) - 2701248..2701718 (-) 471 WP_033709027.1 ArpU family phage packaging/lysis transcriptional regulator -
  BWEI_RS31165 (bwei_2865) - 2701920..2702521 (-) 602 Protein_2797 DUF6944 family repetitive protein -
  BWEI_RS13535 - 2703956..2704234 (+) 279 WP_002127130.1 hypothetical protein -
  BWEI_RS13540 (bwei_2869) - 2705095..2705874 (+) 780 WP_038626174.1 BclA C-terminal domain-containing protein -
  BWEI_RS13545 - 2706386..2707086 (+) 701 Protein_2800 exosporium leader peptide-containing protein -
  BWEI_RS13550 (bwei_2872) - 2707383..2707847 (+) 465 WP_002035352.1 hypothetical protein -
  BWEI_RS13560 (bwei_2875) - 2708133..2709275 (+) 1143 WP_050426684.1 exosporium leader peptide-containing protein -
  BWEI_RS30640 - 2709488..2709706 (+) 219 Protein_2803 hypothetical protein -
  BWEI_RS13565 (bwei_2876) - 2709727..2710863 (+) 1137 WP_002035357.1 site-specific integrase -

Sequence


Protein


Download         Length: 112 a.a.        Molecular weight: 12966.75 Da        Isoelectric Point: 9.1002

>NTDB_id=131785 BWEI_RS13470 WP_002012564.1 2692642..2692980(-) (ssbA) [Bacillus mycoides strain WSBC10204]
MMNRVVLIGRLTKEPELYYTKQGVAYARICVAVNRGFRNSLGEQQVDFINCVVWRRSAENVTEYCTKGSLVGITGRIHTS
NYDDEQGKRVYRTEVLIENITFLERRRESASQ

Nucleotide


Download         Length: 339 bp        

>NTDB_id=131785 BWEI_RS13470 WP_002012564.1 2692642..2692980(-) (ssbA) [Bacillus mycoides strain WSBC10204]
ATGATGAATCGAGTTGTATTAATCGGTAGATTGACAAAGGAGCCAGAATTATACTACACAAAACAAGGTGTCGCTTATGC
ACGAATATGTGTTGCGGTGAATAGAGGTTTTCGAAATAGTTTAGGTGAGCAACAAGTAGATTTTATTAATTGTGTTGTAT
GGAGAAGGTCGGCTGAAAATGTAACAGAATATTGTACGAAAGGCTCACTTGTTGGGATTACAGGGCGTATTCATACGAGT
AATTACGATGATGAACAAGGAAAGAGAGTATATAGAACTGAAGTTCTGATTGAGAATATTACATTTTTAGAGAGAAGGCG
GGAGAGTGCATCGCAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB J8I4L2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

57.547

94.643

0.545

  ssb Latilactobacillus sakei subsp. sakei 23K

53.774

94.643

0.509

  ssbB Bacillus subtilis subsp. subtilis str. 168

50.442

100

0.509

  ssbB Lactococcus lactis subsp. cremoris KW2

43.86

100

0.446

  ssbB Streptococcus sobrinus strain NIDR 6715-7

42.727

98.214

0.42

  ssbB/cilA Streptococcus pneumoniae TIGR4

42.727

98.214

0.42

  ssbB/cilA Streptococcus pneumoniae D39

41.818

98.214

0.411

  ssbB/cilA Streptococcus pneumoniae Rx1

41.818

98.214

0.411

  ssbB/cilA Streptococcus pneumoniae R6

41.818

98.214

0.411

  ssbB/cilA Streptococcus mitis SK321

41.818

98.214

0.411

  ssbB/cilA Streptococcus mitis NCTC 12261

40.909

98.214

0.402

  ssbA Streptococcus mutans UA159

40.909

98.214

0.402


Multiple sequence alignment