Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   BF32_RS26760 Genome accession   NZ_CP009600
Coordinates   5068346..5068684 (+) Length   112 a.a.
NCBI ID   WP_000982029.1    Uniprot ID   A0A437SMJ3
Organism   Bacillus thuringiensis strain HD571     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 5058064..5068684 5068346..5068684 within 0


Gene organization within MGE regions


Location: 5058064..5068684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF32_RS26685 (BF32_5228) - 5058064..5058255 (+) 192 WP_000512844.1 DUF3954 domain-containing protein -
  BF32_RS26690 (BF32_5229) - 5058573..5058890 (-) 318 WP_000722282.1 hypothetical protein -
  BF32_RS26700 (BF32_5231) - 5058953..5060106 (+) 1154 Protein_5102 lipase family protein -
  BF32_RS26710 (BF32_5233) - 5060103..5060336 (+) 234 WP_001249586.1 hypothetical protein -
  BF32_RS30125 (BF32_5234) - 5060905..5061180 (+) 276 WP_001256463.1 hypothetical protein -
  BF32_RS30130 (BF32_5235) - 5061184..5061459 (+) 276 WP_307832946.1 N-acetylmuramoyl-L-alanine amidase -
  BF32_RS26720 (BF32_5236) - 5061518..5061859 (-) 342 WP_000384715.1 hypothetical protein -
  BF32_RS26725 (BF32_5237) - 5062066..5062377 (-) 312 WP_000923399.1 contact-dependent growth inhibition system immunity protein -
  BF32_RS26730 (BF32_5238) - 5062394..5063317 (-) 924 WP_080517984.1 RNase A-like domain-containing protein -
  BF32_RS26735 (BF32_5239) - 5063610..5064371 (-) 762 WP_000499700.1 hypothetical protein -
  BF32_RS26740 (BF32_5240) - 5064481..5065128 (+) 648 WP_000165945.1 HD domain-containing protein -
  BF32_RS26745 (BF32_5241) - 5065125..5067020 (+) 1896 WP_000783196.1 ABC-F family ATP-binding cassette domain-containing protein -
  BF32_RS26750 (BF32_5242) - 5067096..5067827 (+) 732 WP_001260645.1 Bax inhibitor-1/YccA family membrane protein -
  BF32_RS26755 (BF32_5243) - 5067933..5068187 (+) 255 WP_000975124.1 DUF4318 domain-containing protein -
  BF32_RS26760 (BF32_5244) ssbA 5068346..5068684 (+) 339 WP_000982029.1 single-stranded DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 112 a.a.        Molecular weight: 12948.86 Da        Isoelectric Point: 9.4347

>NTDB_id=130589 BF32_RS26760 WP_000982029.1 5068346..5068684(+) (ssbA) [Bacillus thuringiensis strain HD571]
MMNRVVLIGRLTKEPELYYTKQGVAYARVCVAVNRGFRNSLGEQQVDFINCVVWRKSAENVTEYCKKGSLVGITGRIHTR
NYEDDQGKRIYITEVVIESITFLERRREGVSQ

Nucleotide


Download         Length: 339 bp        

>NTDB_id=130589 BF32_RS26760 WP_000982029.1 5068346..5068684(+) (ssbA) [Bacillus thuringiensis strain HD571]
ATGATGAATCGAGTTGTATTAATCGGTAGATTGACAAAGGAGCCAGAATTATACTACACAAAGCAAGGCGTCGCGTATGC
ACGAGTATGTGTTGCGGTGAATAGAGGATTTCGAAATAGTTTAGGTGAACAACAAGTAGATTTTATAAATTGTGTCGTAT
GGCGAAAATCGGCTGAGAATGTAACTGAATATTGTAAGAAAGGGTCTCTTGTTGGAATTACCGGACGTATTCATACGAGG
AATTACGAGGATGATCAAGGAAAGAGAATATATATAACGGAAGTCGTGATTGAGAGCATTACATTTTTGGAGAGAAGGCG
CGAAGGTGTATCGCAATAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A437SMJ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

59.434

94.643

0.563
  ssb Latilactobacillus sakei subsp. sakei 23K

56.604

94.643

0.536
  ssbB Bacillus subtilis subsp. subtilis str. 168

51.887

94.643

0.491
  ssbB Lactococcus lactis subsp. cremoris KW2

48.571

93.75

0.455
  ssbB Streptococcus sobrinus strain NIDR 6715-7

46.226

94.643

0.437
  ssbA Streptococcus mutans UA159

44.34

94.643

0.42
  ssbB/cilA Streptococcus pneumoniae TIGR4

42.453

94.643

0.402
  ssbB/cilA Streptococcus mitis NCTC 12261

42.453

94.643

0.402
  ssbB/cilA Streptococcus pneumoniae D39

41.509

94.643

0.393
  ssbB/cilA Streptococcus pneumoniae R6

41.509

94.643

0.393
  ssbB/cilA Streptococcus pneumoniae Rx1

41.509

94.643

0.393
  ssbB/cilA Streptococcus mitis SK321

41.509

94.643

0.393

Multiple sequence alignment