Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LA59_RS14650 Genome accession   NZ_CP009467
Coordinates   3204248..3204652 (+) Length   134 a.a.
NCBI ID   WP_050936251.1    Uniprot ID   -
Organism   Vibrio harveyi strain ATCC 33843 (392 [MAV])     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3199248..3209652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA59_RS14630 (LA59_14620) pdhR 3201280..3202047 (-) 768 WP_069687466.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  LA59_RS14635 (LA59_14625) ampD 3202452..3203003 (-) 552 WP_069687467.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LA59_RS14640 (LA59_14630) nadC 3203096..3203983 (+) 888 WP_005446259.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LA59_RS14650 (LA59_14640) pilA 3204248..3204652 (+) 405 WP_050936251.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LA59_RS14655 (LA59_14645) pilB 3204652..3206337 (+) 1686 WP_049535467.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LA59_RS14660 (LA59_14650) pilC 3206371..3207594 (+) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  LA59_RS14665 (LA59_14655) pilD 3207667..3208536 (+) 870 WP_069687468.1 A24 family peptidase Machinery gene
  LA59_RS14670 (LA59_14660) coaE 3208537..3209151 (+) 615 WP_017189130.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 13689.67 Da        Isoelectric Point: 6.9451

>NTDB_id=129592 LA59_RS14650 WP_050936251.1 3204248..3204652(+) (pilA) [Vibrio harveyi strain ATCC 33843 (392 [MAV])]
MKTNKQKKQQGFTLIELMIVVAIIGVLSAIAVPAYQNYVAKGEAATALGSLRALVTPAELKLQQDGELSGVVSDLGGSAS
HALGNITTTGTGVSAAVLTFTFTKGSLSGDAITLTKSTSGWVCNDGTTILDNCN

Nucleotide


Download         Length: 405 bp        

>NTDB_id=129592 LA59_RS14650 WP_050936251.1 3204248..3204652(+) (pilA) [Vibrio harveyi strain ATCC 33843 (392 [MAV])]
ATGAAAACGAATAAACAGAAGAAGCAGCAGGGTTTTACTCTAATTGAATTGATGATTGTGGTTGCAATTATTGGCGTGCT
TTCCGCAATCGCAGTTCCCGCTTATCAAAATTATGTAGCAAAAGGGGAAGCAGCTACAGCACTTGGTAGCTTAAGAGCTC
TAGTAACACCTGCAGAGTTAAAGCTACAGCAAGATGGCGAACTATCTGGTGTGGTGTCTGATTTAGGAGGTTCTGCCTCA
CATGCCTTAGGTAATATAACTACAACAGGCACAGGTGTCAGTGCTGCAGTGCTGACATTTACTTTTACGAAAGGCTCTCT
ATCTGGTGACGCGATTACTTTAACTAAGTCAACTTCAGGTTGGGTTTGTAACGACGGTACAACTATCTTAGATAACTGTA
ATTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-129)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

51.181

94.776

0.485
  pilA Pseudomonas aeruginosa PAK

41.304

100

0.425
  pilA Vibrio cholerae strain A1552

38.406

100

0.396
  pilA Vibrio cholerae C6706

38.406

100

0.396
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.406

100

0.396
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.686

100

0.396
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.129

92.537

0.381
  comP Acinetobacter baylyi ADP1

37.405

97.761

0.366

Multiple sequence alignment