Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LA59_RS14655 Genome accession   NZ_CP009467
Coordinates   3204652..3206337 (+) Length   561 a.a.
NCBI ID   WP_049535467.1    Uniprot ID   -
Organism   Vibrio harveyi strain ATCC 33843 (392 [MAV])     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3199652..3211337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA59_RS14630 (LA59_14620) pdhR 3201280..3202047 (-) 768 WP_069687466.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  LA59_RS14635 (LA59_14625) ampD 3202452..3203003 (-) 552 WP_069687467.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LA59_RS14640 (LA59_14630) nadC 3203096..3203983 (+) 888 WP_005446259.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LA59_RS14650 (LA59_14640) pilA 3204248..3204652 (+) 405 WP_050936251.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LA59_RS14655 (LA59_14645) pilB 3204652..3206337 (+) 1686 WP_049535467.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LA59_RS14660 (LA59_14650) pilC 3206371..3207594 (+) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  LA59_RS14665 (LA59_14655) pilD 3207667..3208536 (+) 870 WP_069687468.1 A24 family peptidase Machinery gene
  LA59_RS14670 (LA59_14660) coaE 3208537..3209151 (+) 615 WP_017189130.1 dephospho-CoA kinase -
  LA59_RS14675 (LA59_14665) zapD 3209179..3209919 (+) 741 WP_005446251.1 cell division protein ZapD -
  LA59_RS14680 (LA59_14670) yacG 3209982..3210176 (+) 195 WP_005446249.1 DNA gyrase inhibitor YacG -
  LA59_RS14685 (LA59_14675) rplS 3210543..3210896 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62000.80 Da        Isoelectric Point: 5.2849

>NTDB_id=129593 LA59_RS14655 WP_049535467.1 3204652..3206337(+) (pilB) [Vibrio harveyi strain ATCC 33843 (392 [MAV])]
MLTNLSTVLRQAGILTFSQEESLLEQAKASGISMPEALLSSGFFQSHELTEHLSSIFGLSCTSLSQYEYSSLCQKLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFKELTAAIRRLYGRALGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDHQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKTEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPHCRLPQEPNSQLQHIGITSTED
IFQANPDGCNECTQGYSGRTGIYEVMKFDESLSNALIKGASVHELEKLAIANGMQTLQMSGVEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=129593 LA59_RS14655 WP_049535467.1 3204652..3206337(+) (pilB) [Vibrio harveyi strain ATCC 33843 (392 [MAV])]
ATGCTAACCAACCTCTCAACGGTTCTTCGTCAAGCGGGAATACTGACTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGC
AAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTGCTCAGTTCTGGCTTTTTTCAATCTCACGAATTGACTGAGCACCTGA
GTTCTATCTTTGGACTTTCCTGCACATCACTCAGTCAGTATGAGTACTCGTCGCTGTGCCAAAAGCTTGGCTTACGCGAT
CTGATCACTCGACATAATGCCTTGCCACTCAATCGCACATCTTCCACATTAATACTTGCGGTTGCAGACCCAACTAACTT
GCAAGCGGAAGATGACTTTCGTTTTGCTACCGGCTTACAAGTCGAGCTAGTTCTGGCGGATTTTAAAGAACTCACTGCAG
CCATTCGTCGTTTATATGGTCGTGCACTCGGTCAAGAAAAGTCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCAGGT
CTCGTTGATATTGGTGAGGACGAAATTGAGAACATTGAAGATCTCAGCCAAGATGAATCGCCTGTCAGTCGTTATATTAA
CCAAATTCTGCTCGATGCCGTTCGTAAAGGGGCTTCCGATATTCACTTCGAGCCCTATGAAAACATGTACCGAGTGCGTT
TGCGTTGCGATGGCATCTTGATCGAAATTCAGCAGCCTCCGAGCCATTTAAGCCGTCGCCTCTCAGCCCGAATAAAGATC
CTATCTAAGCTTGATATCGCTGAGCGCCGTTTACCACAGGATGGTCGCATCAAGCTCAAATTAAACCAAGACACCGCGAT
TGACATGCGAGTCTCCACGCTGCCAACTCTGTTTGGGGAGAAAATCGTACTACGCTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAACTCGGATACAGCGATCATCAAAAGCAATTGTATTTAGAAGCACTGCGCCGCCCACAAGGCATGATC
CTAATGACAGGACCAACGGGCAGCGGCAAAACGGTTTCTCTTTACACTGGTTTGAGCATTCTCAATAAAACCGAGATCAA
CATTTCCACCGCAGAAGACCCCGTTGAGATCAACTTACCCGGCATTAACCAAGTTCAAGTTCAACCTAAGATCGGCTTTG
GCTTTGCAGAAGCGCTTCGCTCTTTCTTGCGTCAAGATCCCGATGTAGTCATGGTGGGTGAGATCCGAGATCTCGACACG
GCAGAAATCGCGATCAAAGCGTCACAAACCGGGCACTTAGTCCTTTCTACCCTGCACACCAACTCCGCCGCCGAGACGGT
CATTCGCTTATCAAATATGGGGGTAGAAAGCTTTAACCTCGCATCCTCTCTTAGTTTGATCATCGCTCAGCGTTTAGCTC
GAAAACTTTGTCCACATTGCAGACTACCTCAAGAACCAAACAGCCAACTTCAACATATTGGAATTACCAGCACTGAAGAT
ATCTTCCAAGCGAACCCTGACGGTTGTAACGAATGCACCCAAGGATACTCTGGTCGCACAGGCATTTACGAAGTGATGAA
GTTTGATGAATCCCTGTCAAATGCGCTCATCAAAGGCGCTTCAGTACATGAATTAGAAAAGCTAGCGATTGCCAATGGTA
TGCAGACTCTACAAATGTCCGGTGTTGAAAAGCTCAAACAAGGCGTCACCAGCTTTAGTGAGCTGCAACGCGTTCTCTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

96.257

100

0.963
  pilB Vibrio parahaemolyticus RIMD 2210633

89.305

100

0.893
  pilB Vibrio cholerae strain A1552

73.132

100

0.733
  pilB Acinetobacter baylyi ADP1

48.294

99.287

0.479
  pilB Acinetobacter baumannii D1279779

51.838

92.157

0.478
  pilB Legionella pneumophila strain ERS1305867

47.97

96.613

0.463
  pilF Neisseria gonorrhoeae MS11

42.883

100

0.43
  pilB/pilB1 Synechocystis sp. PCC 6803

37.037

100

0.392
  pilF Thermus thermophilus HB27

36.477

100

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.559

99.465

0.364

Multiple sequence alignment