Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LK03_RS04555 Genome accession   NZ_CP009455
Coordinates   1071257..1072519 (-) Length   420 a.a.
NCBI ID   WP_081951556.1    Uniprot ID   -
Organism   Pseudomonas cremoricolorata strain ND07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1066257..1077519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK03_RS04540 (LK03_04580) - 1067935..1069524 (-) 1590 WP_038411268.1 AAA family ATPase -
  LK03_RS04545 (LK03_04585) aroB 1069540..1070637 (-) 1098 WP_038411269.1 3-dehydroquinate synthase -
  LK03_RS04550 (LK03_04590) aroK 1070734..1071252 (-) 519 WP_038411270.1 shikimate kinase AroK -
  LK03_RS04555 pilQ 1071257..1072519 (-) 1263 WP_081951556.1 secretin N-terminal domain-containing protein Machinery gene
  LK03_RS04560 - 1072449..1073471 (-) 1023 WP_049870426.1 pilus assembly protein PilP -
  LK03_RS04565 (LK03_04605) - 1073468..1074004 (-) 537 WP_038411271.1 PilN domain-containing protein -
  LK03_RS04570 (LK03_04610) pilM 1074004..1074897 (-) 894 WP_038411272.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45944.69 Da        Isoelectric Point: 5.2178

>NTDB_id=129400 LK03_RS04555 WP_081951556.1 1071257..1072519(-) (pilQ) [Pseudomonas cremoricolorata strain ND07]
MRACWTALLILISLHTQAQPAEDERLSLNFQDVEVRAVLQVLAEHAGVNLVASDTVQGRITLRLDQVAWQQALDLVLRSK
GLGMRQEGNVLVVAPAAALLEQATQARLGEMHEAQQQPLQRELVPIHHANAAELAELLLTALAEEPALATRGGLSVDVRT
NTLIVSQPAQRLQELRKLIRQLDVPVRQVMIEARIVEVNVDYEQGLGVRWGSGLYQEGARAGKELFVDLGVERAGSVIGL
GLLRSDVLLDLELSAMEKTGNGEIISQPKVFTADKETARILKGTEVPYQETSKSGATSTSFREASLSLEVTPQITPDGDV
IMTVRVTKDEPDYLNALNNVPPIRKNEVNAKVRVANGDTIVIGGVYSTSQNKVSDKVPFLGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMGDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=129400 LK03_RS04555 WP_081951556.1 1071257..1072519(-) (pilQ) [Pseudomonas cremoricolorata strain ND07]
ATGCGAGCGTGCTGGACAGCGTTGCTGATACTGATCTCCCTGCACACCCAGGCGCAGCCCGCCGAGGATGAGCGGCTGTC
GCTGAACTTCCAGGATGTCGAGGTGCGAGCGGTGCTCCAGGTGCTGGCCGAGCACGCTGGGGTGAACCTGGTGGCCAGCG
ATACCGTGCAGGGGCGCATCACCTTGCGCCTCGATCAGGTGGCCTGGCAGCAGGCCCTCGATCTGGTGTTGCGCAGCAAG
GGGCTGGGCATGCGACAGGAAGGCAACGTGCTGGTGGTCGCCCCTGCTGCAGCGCTCTTGGAGCAGGCTACCCAGGCGCG
GCTGGGCGAGATGCATGAGGCGCAGCAACAGCCTTTGCAGCGTGAACTCGTTCCCATCCATCACGCCAATGCAGCGGAAC
TGGCAGAGCTGCTGCTCACCGCGCTGGCCGAGGAGCCCGCCCTGGCAACCCGCGGGGGTCTCAGCGTGGATGTGCGCACC
AACACCCTGATCGTCAGCCAGCCGGCGCAGCGTCTGCAAGAGCTGCGCAAACTGATCAGGCAACTGGATGTGCCGGTGCG
TCAGGTGATGATCGAAGCGCGCATCGTCGAAGTGAACGTCGACTACGAACAGGGGCTGGGTGTGCGCTGGGGCAGTGGTC
TGTACCAAGAGGGCGCGCGGGCCGGCAAGGAGCTCTTCGTCGATCTGGGTGTGGAGCGAGCGGGGTCGGTGATCGGCCTT
GGGCTACTGCGCAGCGACGTGTTGCTCGACCTGGAACTCAGTGCCATGGAAAAGACCGGCAATGGCGAGATCATCTCCCA
GCCCAAGGTCTTCACCGCCGACAAGGAAACCGCGAGGATTCTCAAGGGCACCGAGGTGCCGTACCAGGAAACCAGCAAGA
GCGGAGCGACCTCGACCTCGTTTCGCGAGGCTTCGCTGTCGCTGGAAGTCACACCGCAGATCACCCCGGATGGCGATGTG
ATCATGACCGTGCGCGTGACCAAGGACGAGCCGGACTACCTCAACGCCCTGAACAACGTACCGCCGATCCGAAAGAACGA
GGTCAACGCCAAGGTTCGCGTCGCCAACGGCGATACCATTGTGATTGGCGGCGTGTATTCGACGTCGCAAAACAAAGTGT
CGGACAAGGTGCCATTTCTCGGTGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTTTTGGTCTTTCTGACTCCGCGTATCATGGGAGACCAGGCGATAGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(255-411)

Secretin and TonB N terminus short domain (STN)

(49-95)

Bacterial type II/III secretion system short domain (Secretin_N)

(123-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

56.221

100

0.581
  comQ Acinetobacter baylyi ADP1

42.152

100

0.448
  pilQ Acinetobacter baumannii D1279779

39.78

100

0.431
  pilQ Acinetobacter baumannii strain A118

39.78

100

0.431
  pilQ Legionella pneumophila strain Lp02

42.169

98.81

0.417
  pilQ Legionella pneumophila strain ERS1305867

42.169

98.81

0.417
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.19

100

0.412
  pilQ Vibrio cholerae strain A1552

41.19

100

0.412
  pilQ Vibrio campbellii strain DS40M4

39.623

100

0.4
  pilQ Neisseria gonorrhoeae strain FA1090

37.198

98.571

0.367
  pilQ Neisseria meningitidis MC58

37.108

98.81

0.367
  pilQ Neisseria meningitidis M1080

37.108

98.81

0.367

Multiple sequence alignment