Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   O165_RS20790 Genome accession   NZ_CP009365
Coordinates   4527997..4529220 (-) Length   407 a.a.
NCBI ID   WP_023629542.1    Uniprot ID   -
Organism   Pseudomonas soli strain SJ10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4522997..4534220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O165_RS20775 (O165_021225) - 4524640..4526265 (-) 1626 WP_038706966.1 AAA family ATPase -
  O165_RS20780 (O165_021230) aroB 4526281..4527378 (-) 1098 WP_023631794.1 3-dehydroquinate synthase -
  O165_RS20785 (O165_021235) aroK 4527474..4527992 (-) 519 WP_038706967.1 shikimate kinase AroK -
  O165_RS20790 (O165_021240) pilQ 4527997..4529220 (-) 1224 WP_023629542.1 type IV pilus secretin PilQ Machinery gene
  O165_RS20795 - 4529270..4530190 (-) 921 WP_052062231.1 pilus assembly protein PilP -
  O165_RS20800 (O165_021250) - 4530187..4530705 (-) 519 WP_160291120.1 PilN domain-containing protein -
  O165_RS20805 (O165_021255) pilM 4530723..4531619 (-) 897 WP_023629540.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44154.70 Da        Isoelectric Point: 4.9166

>NTDB_id=128946 O165_RS20790 WP_023629542.1 4527997..4529220(-) (pilQ) [Pseudomonas soli strain SJ10]
MSNILAVQAAQFQGEPISLNFQDVELRSVLQVMAEHAGVNLVASDSVQGRITLHLEDVPWAQALDLVLRSKGLGRRQEGN
VLLIAPVAELAGQARGMGEEVLLEPLRRELIPVFHAKAAELAELLLASLADDGILTGRGSLSVDARTNTLVVTQPGERLA
DLRRLVAQLDVPVRQVMIEARIVEASVDYEKGLGVRWGGPLYGVDSRLGSDLFVDLGVERATSGLGVGLLRSDILLDLEL
SAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETTRSGATSISFREASLSLEVTPQITPDGKVVMDVRVTKDEPDF
INALNNVPPIRKNEVTAKVRVTDGQTIVIGGVYSTSQNKVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRIMSD
QAIAVSR

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=128946 O165_RS20790 WP_023629542.1 4527997..4529220(-) (pilQ) [Pseudomonas soli strain SJ10]
ATGTCGAACATCCTGGCAGTGCAGGCAGCGCAATTCCAGGGTGAGCCGATATCGCTCAACTTCCAGGATGTCGAACTGCG
CTCAGTGCTTCAGGTCATGGCGGAGCACGCCGGCGTCAACCTGGTCGCCAGCGATTCGGTGCAGGGGCGAATAACCCTGC
ACCTGGAAGACGTGCCTTGGGCCCAGGCACTTGACCTTGTGCTGCGCAGCAAGGGGCTGGGCCGGCGCCAGGAGGGCAAC
GTGCTGTTGATCGCTCCCGTTGCTGAGCTGGCCGGGCAGGCCCGGGGCATGGGCGAGGAGGTGTTGCTGGAGCCTTTGCG
ACGAGAGCTGATCCCTGTCTTTCATGCAAAAGCCGCCGAGCTGGCGGAACTGTTGCTTGCGAGCTTGGCCGATGACGGCA
TCCTGACTGGGCGCGGCAGCCTCAGTGTGGATGCACGGACCAACACGCTGGTGGTTACCCAGCCTGGCGAACGACTTGCC
GATTTGCGAAGGTTGGTAGCGCAGTTGGATGTGCCGGTACGCCAGGTCATGATCGAGGCGCGTATCGTAGAAGCCAGTGT
CGATTACGAGAAGGGCCTGGGTGTGCGATGGGGCGGCCCGTTGTATGGGGTGGACAGTCGCCTGGGTAGTGACCTGTTCG
TCGACCTTGGGGTTGAACGGGCCACTTCCGGGCTCGGCGTGGGCCTGCTGCGCAGCGACATTCTGCTGGATCTGGAGCTC
AGCGCCATGGAGAAGAGCGGCAATGGCGAGATCATCTCCCAGCCCAAGGTGGTCACGGCTGACAAGGAAACCGCCCGCAT
ACTCAAGGGGACCGAAGTACCCTATCAAGAGACCACTCGCAGTGGGGCTACCTCCATTTCTTTTCGTGAGGCCTCCCTGT
CGCTGGAGGTAACGCCGCAGATCACGCCGGATGGCAAGGTAGTGATGGATGTGCGCGTGACCAAGGACGAGCCCGACTTC
ATCAATGCACTCAACAATGTACCGCCGATCCGCAAGAACGAGGTTACGGCGAAGGTCCGGGTGACCGATGGGCAGACCAT
TGTGATTGGCGGCGTGTACTCGACGTCGCAGAACAAAGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTG
GGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGAC
CAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(110-175)

Bacterial type II and III secretion system protein (Secretin)

(242-398)

Secretin and TonB N terminus short domain (STN)

(40-86)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

57.798

100

0.619
  comQ Acinetobacter baylyi ADP1

43.498

100

0.477
  pilQ Legionella pneumophila strain ERS1305867

44.787

100

0.464
  pilQ Acinetobacter baumannii strain A118

40.959

100

0.462
  pilQ Acinetobacter baumannii D1279779

40.959

100

0.462
  pilQ Legionella pneumophila strain Lp02

44.313

100

0.459
  pilQ Vibrio campbellii strain DS40M4

41.038

100

0.428
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.388

100

0.425
  pilQ Vibrio cholerae strain A1552

41.388

100

0.425
  pilQ Neisseria gonorrhoeae strain FA1090

37.081

100

0.381
  pilQ Neisseria meningitidis MC58

36.124

100

0.371
  pilQ Neisseria meningitidis M1080

36.124

100

0.371

Multiple sequence alignment