Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   AS54_RS12200 Genome accession   NZ_CP009318
Coordinates   2151414..2151752 (+) Length   112 a.a.
NCBI ID   WP_000982029.1    Uniprot ID   A0A437SMJ3
Organism   Bacillus cereus 03BB102     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2141132..2151752 2151414..2151752 within 0


Gene organization within MGE regions


Location: 2141132..2151752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS54_RS12125 (AS54_2259) - 2141132..2141323 (+) 192 WP_000512844.1 DUF3954 domain-containing protein -
  AS54_RS12130 (AS54_2260) - 2141642..2141959 (-) 318 WP_000722282.1 hypothetical protein -
  AS54_RS12140 (AS54_2262) - 2142022..2143174 (+) 1153 Protein_2135 lipase family protein -
  AS54_RS12150 (AS54_2263) - 2143171..2143404 (+) 234 WP_001249587.1 hypothetical protein -
  AS54_RS31185 (AS54_2264) - 2143973..2144248 (+) 276 WP_001256463.1 hypothetical protein -
  AS54_RS31190 (AS54_2265) - 2144252..2144527 (+) 276 WP_307832946.1 N-acetylmuramoyl-L-alanine amidase -
  AS54_RS12160 (AS54_2266) - 2144586..2144927 (-) 342 WP_000384715.1 hypothetical protein -
  AS54_RS12165 (AS54_2267) - 2145134..2145445 (-) 312 WP_000923399.1 contact-dependent growth inhibition system immunity protein -
  AS54_RS12170 (AS54_2268) - 2145462..2146385 (-) 924 WP_002002651.1 RNase A-like domain-containing protein -
  AS54_RS12175 (AS54_2269) - 2146678..2147439 (-) 762 WP_000499735.1 hypothetical protein -
  AS54_RS12180 (AS54_2270) - 2147549..2148196 (+) 648 WP_000165945.1 HD domain-containing protein -
  AS54_RS12185 (AS54_2271) - 2148193..2150088 (+) 1896 WP_000783196.1 ABC-F family ATP-binding cassette domain-containing protein -
  AS54_RS12190 (AS54_2272) - 2150164..2150895 (+) 732 WP_001260645.1 Bax inhibitor-1/YccA family membrane protein -
  AS54_RS12195 (AS54_2273) - 2151001..2151255 (+) 255 WP_000975124.1 DUF4318 domain-containing protein -
  AS54_RS12200 (AS54_2274) ssbA 2151414..2151752 (+) 339 WP_000982029.1 single-stranded DNA-binding protein Machinery gene

Sequence


Protein


Download         Length: 112 a.a.        Molecular weight: 12948.86 Da        Isoelectric Point: 9.4347

>NTDB_id=128484 AS54_RS12200 WP_000982029.1 2151414..2151752(+) (ssbA) [Bacillus cereus 03BB102]
MMNRVVLIGRLTKEPELYYTKQGVAYARVCVAVNRGFRNSLGEQQVDFINCVVWRKSAENVTEYCKKGSLVGITGRIHTR
NYEDDQGKRIYITEVVIESITFLERRREGVSQ

Nucleotide


Download         Length: 339 bp        

>NTDB_id=128484 AS54_RS12200 WP_000982029.1 2151414..2151752(+) (ssbA) [Bacillus cereus 03BB102]
ATGATGAATCGAGTTGTATTAATCGGTAGATTGACAAAGGAGCCAGAATTATACTACACAAAGCAAGGCGTCGCGTATGC
ACGAGTATGTGTTGCGGTGAATAGAGGATTTCGAAATAGTTTAGGTGAACAACAAGTAGATTTTATAAATTGTGTCGTAT
GGCGAAAATCGGCTGAGAATGTAACTGAATATTGTAAGAAAGGGTCTCTTGTTGGAATTACCGGACGTATTCATACGAGG
AATTACGAGGATGATCAAGGAAAGAGAATATATATAACGGAAGTCGTGATTGAGAGCATTACATTTTTGGAGAGAAGGCG
CGAAGGTGTATCGCAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A437SMJ3

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

59.434

94.643

0.563

  ssb Latilactobacillus sakei subsp. sakei 23K

56.604

94.643

0.536

  ssbB Bacillus subtilis subsp. subtilis str. 168

51.887

94.643

0.491

  ssbB Lactococcus lactis subsp. cremoris KW2

48.571

93.75

0.455

  ssbB Streptococcus sobrinus strain NIDR 6715-7

46.226

94.643

0.437

  ssbA Streptococcus mutans UA159

44.34

94.643

0.42

  ssbB/cilA Streptococcus pneumoniae TIGR4

42.453

94.643

0.402

  ssbB/cilA Streptococcus mitis NCTC 12261

42.453

94.643

0.402

  ssbB/cilA Streptococcus pneumoniae D39

41.509

94.643

0.393

  ssbB/cilA Streptococcus pneumoniae R6

41.509

94.643

0.393

  ssbB/cilA Streptococcus pneumoniae Rx1

41.509

94.643

0.393

  ssbB/cilA Streptococcus mitis SK321

41.509

94.643

0.393


Multiple sequence alignment