Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   B6116_RS03295 Genome accession   NZ_CP007667
Coordinates   632730..633188 (-) Length   152 a.a.
NCBI ID   WP_002244388.1    Uniprot ID   -
Organism   Neisseria meningitidis strain B6116/77     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 627730..638188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6116_RS03280 (B6116_00616) - 628188..630224 (+) 2037 WP_002244386.1 M3 family metallopeptidase -
  B6116_RS03285 (B6116_00617) - 630646..631212 (-) 567 WP_002220235.1 manganese efflux pump MntP family protein -
  B6116_RS03290 (B6116_00619) - 631676..632176 (-) 501 WP_002226858.1 REP-associated tyrosine transposase -
  B6116_RS13530 - 632213..632500 (-) 288 WP_139619977.1 hypothetical protein -
  B6116_RS03295 (B6116_00620) pilE 632730..633188 (-) 459 WP_002244388.1 pilin Machinery gene
  B6116_RS14370 - 633547..633729 (+) 183 WP_153308415.1 hypothetical protein -
  B6116_RS14375 - 633853..634050 (+) 198 WP_153308414.1 hypothetical protein -
  B6116_RS14380 - 634137..634274 (-) 138 WP_153308413.1 hypothetical protein -
  B6116_RS03310 (B6116_00623) katA 634350..635864 (-) 1515 WP_002239455.1 catalase KatA -
  B6116_RS03315 (B6116_00624) - 636212..637063 (+) 852 WP_002244390.1 RNA polymerase factor sigma-54 -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15934.28 Da        Isoelectric Point: 9.3582

>NTDB_id=122188 B6116_RS03295 WP_002244388.1 632730..633188(-) (pilE) [Neisseria meningitidis strain B6116/77]
MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEALTLAEGQKSAVVEYYSDNGTFPANNTSAGIAASSTIT
GKYVSSVAVTAEASGNASIAKITAKMKKNGVNKDISEKTLVLVGKQNSGSFSWTCQKGDVDEKFLPSSCRAK

Nucleotide


Download         Length: 459 bp        

>NTDB_id=122188 B6116_RS03295 WP_002244388.1 632730..633188(-) (pilE) [Neisseria meningitidis strain B6116/77]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAGCTGATGATCGTCATCGCCATCGTCGGTATCTTGGCAGCCGTCGC
CCTGCCCGCATACCAAGACTACACCGCGCGCGCCCAAATGTCCGAAGCCCTGACTTTGGCGGAAGGTCAAAAATCCGCAG
TGGTCGAGTATTATTCCGACAACGGTACGTTCCCTGCCAACAATACTTCCGCAGGTATTGCTGCTTCTAGTACAATTACA
GGTAAATATGTGAGTTCTGTAGCAGTTACAGCGGAAGCATCTGGCAATGCATCTATTGCCAAGATTACCGCCAAAATGAA
AAAAAATGGTGTGAACAAAGATATTTCAGAAAAAACTTTGGTGCTGGTGGGTAAACAAAACAGTGGTTCGTTCTCTTGGA
CTTGTCAAAAAGGCGATGTGGACGAAAAATTCCTGCCGTCTTCCTGCCGCGCCAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

57.229

100

0.625
  pilE Neisseria gonorrhoeae MS11

53.846

100

0.599
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.458

100

0.461
  pilA2 Legionella pneumophila strain ERS1305867

44.295

98.026

0.434
  pilA2 Legionella pneumophila str. Paris

43.333

98.684

0.428
  comP Acinetobacter baylyi ADP1

41.176

100

0.414
  pilA/pilA1 Eikenella corrodens VA1

38.994

100

0.408
  pilA Ralstonia pseudosolanacearum GMI1000

37.126

100

0.408
  pilA Acinetobacter baumannii strain A118

40.132

100

0.401
  pilA Acinetobacter nosocomialis M2

38.667

98.684

0.382
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.538

100

0.375

Multiple sequence alignment