Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CH92_RS16660 Genome accession   NZ_CP007441
Coordinates   3667065..3668282 (-) Length   405 a.a.
NCBI ID   WP_025242900.1    Uniprot ID   W8RX81
Organism   Stutzerimonas stutzeri strain 28a24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3662065..3673282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH92_RS16630 (CH92_16985) rplM 3662263..3662691 (-) 429 WP_019342644.1 50S ribosomal protein L13 -
  CH92_RS16635 (CH92_16990) - 3663085..3664122 (+) 1038 WP_025242895.1 NADP(H)-dependent aldo-keto reductase -
  CH92_RS16640 (CH92_16995) - 3664229..3664921 (+) 693 WP_025242896.1 energy-coupling factor ABC transporter permease -
  CH92_RS16645 (CH92_17000) yacG 3664973..3665170 (-) 198 WP_025242897.1 DNA gyrase inhibitor YacG -
  CH92_RS16650 (CH92_17005) coaE 3665167..3665775 (-) 609 WP_025242898.1 dephospho-CoA kinase -
  CH92_RS16655 (CH92_17010) pilD 3666196..3667062 (-) 867 WP_025242899.1 A24 family peptidase Machinery gene
  CH92_RS16660 (CH92_17015) pilC 3667065..3668282 (-) 1218 WP_025242900.1 type II secretion system F family protein Machinery gene
  CH92_RS16665 (CH92_17020) pilB 3668284..3669990 (-) 1707 WP_025242901.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CH92_RS16670 (CH92_17025) pilE 3670216..3670683 (+) 468 WP_025242902.1 pilin Machinery gene
  CH92_RS16675 (CH92_17030) - 3671881..3672399 (+) 519 WP_038623084.1 transposase -
  CH92_RS16680 (CH92_17040) - 3672689..3673126 (+) 438 WP_025242903.1 YtoQ family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43965.67 Da        Isoelectric Point: 9.8511

>NTDB_id=119361 CH92_RS16660 WP_025242900.1 3667065..3668282(-) (pilC) [Stutzerimonas stutzeri strain 28a24]
MAQKAVKTSVFTWEGTNRQGAKIKGELSGVSPALVKAQLRKQGVNPQKVRKKSVSLFSAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDDLKQEVAAGNSFATALRKKPQYFDDLYCNLVDSGEQSGSLETLLDRVATYKEKT
EALKAKIKKAMNYPIAVVLVAIIVSAILLIKVVPQFQDVFANFGAELPAFTLFVIGISEALQAWWHLVLLAMVGAAYAFK
TAHGKSEGFRNWFDRFLLKMPIVGDILYKSAVARFARTLATTFAAGVPLVDALDSVAGATGNVVFRNATMKVKSDVSSGM
QLNFSMRTTGTFPSMAIQMTAIGEESGSLDEMLAKVATFYEDEVDNMVDGLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=119361 CH92_RS16660 WP_025242900.1 3667065..3668282(-) (pilC) [Stutzerimonas stutzeri strain 28a24]
ATGGCGCAGAAAGCGGTAAAAACCAGTGTCTTCACCTGGGAAGGGACGAACAGACAGGGCGCAAAGATAAAAGGCGAGCT
GAGCGGCGTAAGCCCCGCACTGGTCAAGGCGCAGTTGCGCAAACAGGGCGTCAATCCGCAGAAGGTGCGCAAGAAGTCGG
TGTCGCTGTTCAGCGCCGGCAAAAAGATCAAGCCCATGGACATCGCCCTGTTTACACGGCAGATGGCAACCATGATGAAA
GCCGGCGTTCCGCTGCTGCAGTCGTTCGACATCATTGGTGAGGGTTTCGATAACCCGAACATGCGCAAGCTGGTCGACGA
CCTCAAGCAGGAGGTTGCCGCGGGTAACAGCTTCGCGACGGCGCTGCGTAAGAAACCTCAATACTTCGATGATCTCTATT
GCAACCTGGTTGATTCGGGCGAGCAGTCCGGCTCTCTGGAAACCTTGTTGGATCGCGTCGCGACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAAGCCATGAATTACCCCATCGCGGTGGTGCTGGTTGCGATCATCGTGTCGGCCAT
TCTGTTGATCAAGGTGGTCCCACAGTTTCAGGACGTGTTCGCCAACTTTGGTGCGGAACTCCCTGCGTTTACATTGTTTG
TAATTGGCATATCCGAAGCGCTGCAGGCGTGGTGGCATCTGGTGTTGCTTGCCATGGTTGGCGCTGCTTACGCCTTCAAG
ACGGCCCACGGCAAGTCCGAGGGTTTTCGTAACTGGTTTGACCGATTCCTTCTCAAAATGCCGATCGTGGGTGACATCCT
CTACAAGTCAGCGGTGGCGCGCTTCGCGCGGACCCTGGCGACCACCTTCGCGGCGGGTGTGCCTCTAGTCGATGCGTTGG
ATTCGGTCGCCGGAGCTACTGGCAATGTGGTGTTCCGCAACGCGACGATGAAGGTCAAGAGTGATGTGTCCAGCGGTATG
CAGCTGAACTTCTCCATGCGCACCACTGGCACATTCCCGAGCATGGCGATCCAGATGACCGCTATTGGCGAAGAGTCCGG
CTCGCTGGATGAAATGCTGGCCAAGGTTGCGACCTTCTATGAAGACGAAGTGGACAACATGGTCGATGGGCTGACAGCGT
TGATGGAGCCGATGATCATGGCGGTTCTTGGTGTGCTGGTTGGCGGCTTGATCATCGCCATGTACCTGCCGATCTTCCAG
TTGGGAGCTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8RX81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

91.358

100

0.914
  pilC Acinetobacter baylyi ADP1

59.36

100

0.595
  pilC Acinetobacter baumannii D1279779

59.012

100

0.59
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilG Neisseria gonorrhoeae MS11

45.274

99.259

0.449
  pilG Neisseria meningitidis 44/76-A

45.274

99.259

0.449
  pilC Vibrio cholerae strain A1552

42.929

97.778

0.42
  pilC Vibrio campbellii strain DS40M4

41.162

97.778

0.402
  pilC Thermus thermophilus HB27

37.343

98.519

0.368

Multiple sequence alignment