Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CH92_RS16665 Genome accession   NZ_CP007441
Coordinates   3668284..3669990 (-) Length   568 a.a.
NCBI ID   WP_025242901.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain 28a24     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3663284..3674990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH92_RS16640 (CH92_16995) - 3664229..3664921 (+) 693 WP_025242896.1 energy-coupling factor ABC transporter permease -
  CH92_RS16645 (CH92_17000) yacG 3664973..3665170 (-) 198 WP_025242897.1 DNA gyrase inhibitor YacG -
  CH92_RS16650 (CH92_17005) coaE 3665167..3665775 (-) 609 WP_025242898.1 dephospho-CoA kinase -
  CH92_RS16655 (CH92_17010) pilD 3666196..3667062 (-) 867 WP_025242899.1 A24 family peptidase Machinery gene
  CH92_RS16660 (CH92_17015) pilC 3667065..3668282 (-) 1218 WP_025242900.1 type II secretion system F family protein Machinery gene
  CH92_RS16665 (CH92_17020) pilB 3668284..3669990 (-) 1707 WP_025242901.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CH92_RS16670 (CH92_17025) pilE 3670216..3670683 (+) 468 WP_025242902.1 pilin Machinery gene
  CH92_RS16675 (CH92_17030) - 3671881..3672399 (+) 519 WP_038623084.1 transposase -
  CH92_RS16680 (CH92_17040) - 3672689..3673126 (+) 438 WP_025242903.1 YtoQ family protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62013.00 Da        Isoelectric Point: 5.2276

>NTDB_id=119362 CH92_RS16665 WP_025242901.1 3668284..3669990(-) (pilB) [Stutzerimonas stutzeri strain 28a24]
MTDNVALSGLAKQMVLTGLLDDKTAQQAQQQAVRNKLSLVTYVVQNKLANGRAVAELAAEQFGVAYMDLNALDKESQLKD
VVSEKLARQHRVLPLIKRGHKLFVAVSDPTNQQAVTDIQFSTGLTTEAILVEDDKLGLALDKFFESATSGLEDLGDVDLD
GVDVQTVSDDDSANDGAGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAFRVRLRTDGILHEVARPPIQLAPRISA
RLKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALKQP
QGMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCGSCKKEVELPREALMEEGF
PADKIGTFKVYGPVGCENCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=119362 CH92_RS16665 WP_025242901.1 3668284..3669990(-) (pilB) [Stutzerimonas stutzeri strain 28a24]
ATGACCGACAACGTTGCCCTTTCCGGCCTGGCCAAGCAGATGGTGCTAACCGGCCTGCTCGACGACAAGACTGCACAGCA
AGCGCAACAGCAGGCGGTGCGTAACAAGCTGTCACTTGTGACCTATGTTGTGCAGAACAAACTCGCCAATGGCCGGGCGG
TGGCTGAGCTGGCGGCTGAGCAGTTTGGGGTTGCATACATGGACCTCAACGCGCTGGATAAGGAAAGCCAATTAAAGGAC
GTTGTGTCTGAAAAATTGGCGCGTCAGCACCGTGTATTGCCGCTGATAAAACGTGGCCACAAGCTGTTTGTAGCCGTGTC
GGATCCGACCAATCAGCAGGCTGTCACGGATATCCAGTTCAGCACCGGGCTGACGACCGAGGCGATCCTGGTCGAGGACG
ACAAGCTCGGGTTGGCCCTCGACAAGTTTTTTGAAAGCGCGACCTCCGGCCTGGAGGATCTCGGTGACGTGGACCTGGAC
GGGGTCGACGTTCAAACCGTATCGGATGATGATTCAGCGAATGATGGTGCAGGCGAGGCCGCCGACGACGCGCCGGTGGT
TCGCTTCGTCAACAAGATGCTGCTCGATGCCATTCGTGGCGGATCCTCCGACCTGCACTTCGAGCCTTACGAAAAAGCAT
TTCGCGTACGGCTGCGTACGGATGGCATCCTGCATGAAGTCGCGCGGCCTCCGATCCAGCTTGCGCCCCGTATCTCTGCA
CGTCTAAAAGTCATGGCCGGCCTCGATATATCCGAGCGGCGCAAGCCGCAGGACGGCCGGATCAAGATGAAGCTGTCCAA
GACCAAGGCCATCGACTTCCGCGTGAACACGCTGCCGACCTTGTGGGGCGAAAAGATCGTGATGCGGATTCTCGATCCGT
CCAGTGCCCAGATGGGCATCGATGCGCTGGGTTACGAAGAGGATCAGAAAGAGCTGTACATGAATGCCCTCAAGCAACCA
CAGGGCATGATTCTGGTAACCGGGCCGACCGGATCGGGAAAGACCGTTTCGCTTTACACCGGCTTGAATATTCTCAACAC
GGTAGACGTGAATATCTCCACTGCTGAAGACCCAGTGGAAATCAACCTGGAGGGCATCAACCAGGTCAACGTCAACCCTC
GCCAAGGCATGGACTTCTCTCAGGCGCTACGTGCATTCCTGCGCCAGGACCCGGACATCATCATGGTGGGTGAGATTCGT
GACCTGGAAACCGCCGAAATCGCCATCAAGGCGGCTCAGACGGGTCATATGGTGATGTCTACCTTACACACCAACAGCGC
CGCGGAAACGCTGACGCGACTTCGGAACATGGGCGTTGCGGCTTTCAACATCGCCACCTCGGTAAACCTGATTATCGCCC
AGCGCCTTGCGCGCAAGCTCTGCGGTAGCTGCAAGAAAGAAGTGGAGTTGCCGCGCGAGGCATTAATGGAAGAGGGCTTC
CCTGCCGATAAGATCGGCACCTTCAAAGTTTACGGCCCGGTGGGCTGCGAAAACTGCAAGGGCGGATACAAAGGCCGTGT
CGGTATTTATGAAGTGGTTAAAAACACGCCGGCCCTGGCCAGGATTATCATGGAGGACGGCAACTCCATCGACATTTCCA
CCCAGATGCGTAAAGACGGCTTCAACGATCTGCGTACATCGGCTCTTGTAAAAGCCATGCAGGGCGTGACCAGCCTGGAA
GAAGTCAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.331

100

0.593
  pilB Acinetobacter baylyi ADP1

58.333

99.296

0.579
  pilB Legionella pneumophila strain ERS1305867

55.148

100

0.556
  pilB Vibrio parahaemolyticus RIMD 2210633

48.854

99.824

0.488
  pilF Neisseria gonorrhoeae MS11

52.207

91.725

0.479
  pilB Vibrio cholerae strain A1552

49.724

95.599

0.475
  pilB Vibrio campbellii strain DS40M4

47.695

99.296

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

39.579

92.077

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

93.134

0.364

Multiple sequence alignment