Detailed information NTG0119360

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CH92_RS16655
• Genome accession: NZ_CP007441
• Coordinates: 3666196..3667062 (-)
• Length: 288 a.a.
• NCBI ID: WP_025242899.1
• Uniprot ID: W8R1D1
• Organism: Stutzerimonas stutzeri strain 28a24
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3661196..3672062

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CH92_RS16625 (CH92_16980)	rpsI	3661856..3662248 (-)	393	WP_021209313.1	30S ribosomal protein S9	-
CH92_RS16630 (CH92_16985)	rplM	3662263..3662691 (-)	429	WP_019342644.1	50S ribosomal protein L13	-
CH92_RS16635 (CH92_16990)	-	3663085..3664122 (+)	1038	WP_025242895.1	NADP(H)-dependent aldo-keto reductase	-
CH92_RS16640 (CH92_16995)	-	3664229..3664921 (+)	693	WP_025242896.1	energy-coupling factor ABC transporter permease	-
CH92_RS16645 (CH92_17000)	yacG	3664973..3665170 (-)	198	WP_025242897.1	DNA gyrase inhibitor YacG	-
CH92_RS16650 (CH92_17005)	coaE	3665167..3665775 (-)	609	WP_025242898.1	dephospho-CoA kinase	-
CH92_RS16655 (CH92_17010)	pilD	3666196..3667062 (-)	867	WP_025242899.1	A24 family peptidase	Machinery gene
CH92_RS16660 (CH92_17015)	pilC	3667065..3668282 (-)	1218	WP_025242900.1	type II secretion system F family protein	Machinery gene
CH92_RS16665 (CH92_17020)	pilB	3668284..3669990 (-)	1707	WP_025242901.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CH92_RS16670 (CH92_17025)	pilE	3670216..3670683 (+)	468	WP_025242902.1	pilin	Machinery gene

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31801.85 Da
• Isoelectric Point: 6.9991

>NTDB_id=119360 CH92_RS16655 WP_025242899.1 3666196..3667062(-) (pilD) [Stutzerimonas stutzeri strain 28a24]
MDVVTFLASNTLAFVLCTLVLGLLVGSFLNVVIHRLPIMMQRDWRAQAREFLELPAEPAATFNLILPNSHCPHCDHEIRP
WENIPLASWLALRGKCSSCRAPIAKRYPLIELLCGLLSAYVACHFGFSWQAGAMLLLTWGLLAMSMIDVDHQLLPDSIVL
PLLWLGLIVNQFGLFATLPDALWGAIAGYLSLWLVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSVVGA
VLGTIMLRVQKADSGTAIPFGPYLAIAGWIALLWGDRITSSYLQFAQF

Nucleotide

• Length: 867 bp

>NTDB_id=119360 CH92_RS16655 WP_025242899.1 3666196..3667062(-) (pilD) [Stutzerimonas stutzeri strain 28a24]
ATGGATGTGGTGACTTTTCTGGCCAGCAATACGCTGGCCTTTGTTTTATGCACGTTGGTGCTCGGCCTATTGGTTGGCAG
TTTTCTCAACGTCGTAATCCATCGCCTGCCGATCATGATGCAGCGCGATTGGCGCGCTCAGGCTCGAGAGTTTCTGGAAC
TGCCTGCCGAGCCAGCTGCGACATTTAATCTGATCCTTCCAAATTCCCATTGCCCGCATTGCGATCACGAGATACGCCCG
TGGGAGAACATCCCGTTGGCGAGCTGGCTGGCGTTGCGCGGCAAGTGTTCTTCTTGCCGCGCGCCGATCGCTAAGCGCTA
CCCGTTGATCGAACTGCTCTGCGGGCTGTTGTCCGCCTACGTCGCCTGTCATTTCGGTTTTTCCTGGCAGGCTGGTGCGA
TGTTGCTTCTGACCTGGGGGTTGCTGGCGATGAGCATGATTGATGTCGATCATCAGCTGTTGCCCGACTCCATCGTGCTG
CCGCTGCTTTGGCTGGGGTTGATCGTCAATCAGTTCGGGCTGTTTGCTACGTTGCCTGACGCGCTTTGGGGGGCGATTGC
CGGCTATCTCAGTCTTTGGTTGGTGTACTGGCTGTTCAAGCTGGTGACTGGCAAAGAGGGCATGGGTTACGGCGATTTCA
AGTTGCTGGCGATGCTAGGAGCTTGGGGCGGCTGGCAGGTTTTGCCGCTGACCATCCTGCTGTCATCTGTAGTGGGCGCG
GTGTTGGGTACGATCATGCTGCGTGTGCAGAAGGCCGATAGCGGCACCGCCATTCCTTTTGGGCCGTATCTGGCGATCGC
TGGTTGGATCGCGCTGCTGTGGGGTGACCGGATCACCTCAAGCTATCTTCAATTCGCCCAGTTCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (136-243)

3D structure

• AlphaFold DB: W8R1D1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	59.191	94.444	0.559
pilD	Vibrio campbellii strain DS40M4	55.311	94.792	0.524
pilD	Acinetobacter nosocomialis M2	49.648	98.611	0.49
pilD	Acinetobacter baumannii D1279779	49.296	98.611	0.486
pilD	Neisseria gonorrhoeae MS11	52.874	90.625	0.479