Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACMTA2_RS27975 Genome accession   NZ_OZ221631
Coordinates   6344427..6345644 (+) Length   405 a.a.
NCBI ID   WP_413792676.1    Uniprot ID   -
Organism   Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6339427..6350644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMTA2_RS27955 - 6340845..6341141 (+) 297 WP_413792673.1 DUF2845 domain-containing protein -
  ACMTA2_RS27960 - 6341440..6341793 (-) 354 WP_411385653.1 BON domain-containing protein -
  ACMTA2_RS27965 - 6342080..6342499 (-) 420 WP_413792674.1 pilin -
  ACMTA2_RS27970 pilB 6342724..6344424 (+) 1701 WP_413792675.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMTA2_RS27975 pilC 6344427..6345644 (+) 1218 WP_413792676.1 type II secretion system F family protein Machinery gene
  ACMTA2_RS27980 pilD 6345646..6346515 (+) 870 WP_413792677.1 prepilin peptidase Machinery gene
  ACMTA2_RS27985 coaE 6346515..6347138 (+) 624 WP_413792678.1 dephospho-CoA kinase -
  ACMTA2_RS27990 yacG 6347135..6347335 (+) 201 WP_041476151.1 DNA gyrase inhibitor YacG -
  ACMTA2_RS27995 - 6347434..6347649 (-) 216 WP_092333756.1 hypothetical protein -
  ACMTA2_RS28000 - 6347713..6348399 (-) 687 WP_413792679.1 energy-coupling factor ABC transporter permease -
  ACMTA2_RS28005 - 6348484..6349998 (-) 1515 WP_413792680.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44207.97 Da        Isoelectric Point: 10.0551

>NTDB_id=1170556 ACMTA2_RS27975 WP_413792676.1 6344427..6345644(+) (pilC) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
MAVKAVKTDVYTWEGKDRKGAKMTGELTGQSPALVKAQLRKQGINPQKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNANMRKLVDEVKQEVAAGNSFAASLRKCPQYFDDLYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPTAVILVAAVVTGILLVKVVPQFESVFSGLGAQLPAFTVMVIGLSEFMQQWWWMLLGGLAGAFFGVK
YALKRSQAFRDWRDKWLLKLPLIGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIRQDVSTGM
QLNFSMRASGIFPNLAIQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVILGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1170556 ACMTA2_RS27975 WP_413792676.1 6344427..6345644(+) (pilC) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
ATGGCGGTCAAGGCAGTGAAAACCGACGTCTATACGTGGGAAGGCAAAGACCGCAAAGGCGCGAAAATGACTGGCGAACT
GACCGGCCAGAGCCCGGCCCTGGTCAAGGCACAACTGCGCAAACAAGGCATCAACCCGCAGAAAGTGCGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCGCTGGACATTGCCCTCTTCACCCGTCAGATGGCAACGATGCTCAAG
GCTGGCGTGCCGCTGTTGCAGGCGTTCGACATCATCGGAGAAGGCTTCGACAACGCCAACATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAGGTCGCCGCGGGCAACAGCTTCGCCGCCTCGCTGCGCAAATGCCCGCAGTACTTCGACGATTTGTACT
GCAACCTGGTGGATGCCGGTGAACAGGCCGGTGCCCTGGATACACTGTTGGATCGGGTGGCGACCTACAAGGAAAAGAGC
GAAGCCCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCGACGGCGGTGATACTCGTCGCAGCGGTCGTCACTGGCAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCGAATCGGTCTTTTCAGGGCTCGGCGCACAGCTACCGGCCTTCACGGTGATGG
TCATCGGTCTGTCGGAATTCATGCAGCAATGGTGGTGGATGCTGCTGGGCGGCTTGGCCGGGGCGTTTTTCGGGGTGAAA
TATGCCCTCAAGCGTTCCCAGGCGTTTCGTGACTGGCGAGACAAGTGGCTGCTCAAGCTCCCCTTGATCGGCACCTTGAT
GTACAAGTCCGCGGTGGCGCGCTTTGCTCGCACGCTCTCGACCACATTCGCCGCTGGCGTGCCGCTGGTCGAAGCCCTCG
ATTCGGTATCGGGCGCCACCGGCAACGTGGTGTTCAAGCGCGCCGTGCAGCGCATCCGCCAGGATGTCTCCACCGGCATG
CAACTGAATTTCTCCATGCGCGCGTCAGGGATCTTTCCGAACCTGGCCATCCAGATGACCGCCATCGGCGAGGAATCCGG
CGCGCTGGATGACATGCTCGACAAAGTGGCGAGTTTTTATGAAGCCGAAGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGATCCTGGGGGTCGTCGTGGGTGGCCTGGTGGTCGCCATGTACCTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baylyi ADP1

59.548

98.272

0.585
  pilC Acinetobacter baumannii D1279779

57.598

100

0.58
  pilC Legionella pneumophila strain ERS1305867

55.051

97.778

0.538
  pilG Neisseria gonorrhoeae MS11

43.532

99.259

0.432
  pilG Neisseria meningitidis 44/76-A

43.035

99.259

0.427
  pilC Vibrio cholerae strain A1552

43.434

97.778

0.425
  pilC Vibrio campbellii strain DS40M4

43.182

97.778

0.422
  pilC Thermus thermophilus HB27

37.406

99.012

0.37

Multiple sequence alignment