Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMTA2_RS27970 Genome accession   NZ_OZ221631
Coordinates   6342724..6344424 (+) Length   566 a.a.
NCBI ID   WP_413792675.1    Uniprot ID   -
Organism   Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6337724..6349424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMTA2_RS27950 - 6337910..6340843 (+) 2934 WP_413792672.1 DUF748 domain-containing protein -
  ACMTA2_RS27955 - 6340845..6341141 (+) 297 WP_413792673.1 DUF2845 domain-containing protein -
  ACMTA2_RS27960 - 6341440..6341793 (-) 354 WP_411385653.1 BON domain-containing protein -
  ACMTA2_RS27965 - 6342080..6342499 (-) 420 WP_413792674.1 pilin -
  ACMTA2_RS27970 pilB 6342724..6344424 (+) 1701 WP_413792675.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMTA2_RS27975 pilC 6344427..6345644 (+) 1218 WP_413792676.1 type II secretion system F family protein Machinery gene
  ACMTA2_RS27980 pilD 6345646..6346515 (+) 870 WP_413792677.1 prepilin peptidase Machinery gene
  ACMTA2_RS27985 coaE 6346515..6347138 (+) 624 WP_413792678.1 dephospho-CoA kinase -
  ACMTA2_RS27990 yacG 6347135..6347335 (+) 201 WP_041476151.1 DNA gyrase inhibitor YacG -
  ACMTA2_RS27995 - 6347434..6347649 (-) 216 WP_092333756.1 hypothetical protein -
  ACMTA2_RS28000 - 6347713..6348399 (-) 687 WP_413792679.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62715.70 Da        Isoelectric Point: 5.4270

>NTDB_id=1170555 ACMTA2_RS27970 WP_413792675.1 6342724..6344424(+) (pilB) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
MNDIALSGLTKQLVMAELLTEQSAQQAYQQAQRSRIPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKEAQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTRQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRVRTDGMLQEVAKPPIQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLVRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKKSIEIPEETLLKEGFPR
ERIGTFTIYEPVGCQQCNHGYKGRVGVYEVVRNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1170555 ACMTA2_RS27970 WP_413792675.1 6342724..6344424(+) (pilB) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
ATGAACGACATCGCCCTCAGCGGTCTGACCAAGCAATTGGTGATGGCCGAACTGCTCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCCCAACGCAGTCGCATCCCCCTGGTCAGCTACCTGGTGCAGAACAAACTGGTCCAAAGCCGCCAGGTCG
CGGAGATCGCCTCGGAGCATTTCGGCGTGGCCCTGCTGGACCTCAACAGCTTGGACAAGGAAGCCCAGCCCACGGGGCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCACTGTGGCGGCGCGGCAACAAGCTGTTCGTTGGCATTTCCGA
CCCTACCAACCACCAGGCCATCAACGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTCTTCGAATCCAGTAGCACGGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
CTGGACATCGAATCGATCGACGACACCAGGCAGGATTCCATCGCCGGCCAGGACACCGACGACGCGCCCGTGGTGCGCTT
CGTCAACAAGATGTTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAGAGCTATCGCG
TACGAGTGCGCACCGACGGCATGCTGCAGGAAGTGGCCAAGCCACCGATCCAATTGGCGACCCGCATCGCCGCCCGCCTC
AAGGTCATGGCCAGCCTGGATATTTCCGAGCGACGCAAGCCACAGGATGGCCGGCTGAAAATGCGTCTGTCGAAAACCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAGGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTGGGCTACGAACCGGAACAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCGCAAGGG
CTGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGGCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAAGTCAACGTGAACCCGCGCCAGG
GGCTCGACTTTGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAAGCCGCCCAGACCGGGCACCTGGTGCTCTCCACCTTGCACACCAACAGCGCCGCCGA
AACCCTGGTCCGCTTGCAGAACATGGGCATTCCGGGGTTCAACATCGCCACTGCCGTGCACCTGATCATCGCCCAGCGGC
TGGCGCGCAAGCTGTGCAACCACTGCAAGAAGAGCATCGAGATTCCCGAGGAAACCTTGCTCAAGGAAGGTTTCCCCCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCCAACAGTGCAACCACGGGTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAGGAACACGCCCGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGCTTCAATGACCTGCGCACATCGGGGCTGCTCAAAGTGATGCAGGGCGTTACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.127

100

0.581
  pilB Acinetobacter baumannii D1279779

56.966

100

0.571
  pilB Legionella pneumophila strain ERS1305867

54.85

100

0.549
  pilB Vibrio cholerae strain A1552

50.265

99.823

0.502
  pilB Vibrio campbellii strain DS40M4

48.421

100

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

48.677

100

0.488
  pilF Neisseria gonorrhoeae MS11

48.135

99.47

0.479

Multiple sequence alignment