Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   U9J37_RS10180 Genome accession   NZ_OY808997
Coordinates   2232437..2234098 (-) Length   553 a.a.
NCBI ID   WP_005474140.1    Uniprot ID   -
Organism   Vibrio sp. 16 isolate VDT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2227437..2239098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9J37_RS10160 (VDT1_2074) - 2228151..2228978 (-) 828 WP_005474179.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  U9J37_RS10165 (VDT1_2075) - 2229046..2230530 (-) 1485 WP_322413810.1 SPOR domain-containing protein -
  U9J37_RS10170 (VDT1_2076) aroB 2230628..2231716 (-) 1089 WP_005474138.1 3-dehydroquinate synthase -
  U9J37_RS10175 (VDT1_2077) aroK 2231742..2232260 (-) 519 WP_005474150.1 shikimate kinase AroK -
  U9J37_RS10180 (VDT1_2078) pilQ 2232437..2234098 (-) 1662 WP_005474140.1 type IV pilus secretin PilQ Machinery gene
  U9J37_RS10185 (VDT1_2079) pilP 2234159..2234671 (-) 513 WP_005474229.1 pilus assembly protein PilP Machinery gene
  U9J37_RS10190 (VDT1_2080) pilO 2234661..2235251 (-) 591 WP_005474200.1 type 4a pilus biogenesis protein PilO Machinery gene
  U9J37_RS10195 (VDT1_2081) pilN 2235244..2235831 (-) 588 WP_005474158.1 PilN domain-containing protein Machinery gene
  U9J37_RS10200 (VDT1_2082) pilM 2235821..2236816 (-) 996 WP_005474188.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 553 a.a.        Molecular weight: 60510.08 Da        Isoelectric Point: 4.4273

>NTDB_id=1161979 U9J37_RS10180 WP_005474140.1 2232437..2234098(-) (pilQ) [Vibrio sp. 16 isolate VDT1]
MVICALVLLPTSYAQQAMNQLNSIDFRVDSQQNGKIVVELATPHIAVDIHKAQEGLSIELMDTSVSDDKLYVLDVKDFAT
NVSSIEVFRKEDRVILLTSVDGDYGYDYHLTGNYLELVVTQPKVETKLQEKSILEKSGKKISINFQDIPVRNVLQLIADY
NDFNLVVSDSVQGNLTLRLDGVPWQQVLDIILQVKGLDKRIDGNVVLVAPKAELDLREQQMLEKSRLEEELGDLSSEIIK
IKFAKASEIAEMIGGQGAASMLSERGSLTVDERTNALLIRDLKENIDVIRDIIDTLDIPVKQVQIEARIVSVNEGNLDEL
GVRWGFTSTNGNNTVGGSIESNLFQAGLLEGDNLPVDDFLNVNLGATSATASSIAFQVAKLGSDLLLDLELSALQRESKA
EVISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQITPDNRLVLDLNVTQDRPGDVVKTGTGEA
VAINTQRIGTQVLVENGETVVLGGIFQHSVTDTVDKVPLLGDLPLLGALFRRSYQQMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1662 bp        

>NTDB_id=1161979 U9J37_RS10180 WP_005474140.1 2232437..2234098(-) (pilQ) [Vibrio sp. 16 isolate VDT1]
ATGGTTATTTGTGCACTGGTGCTATTGCCGACCAGTTATGCTCAGCAGGCTATGAATCAATTAAATTCTATAGACTTTCG
GGTTGATTCGCAGCAAAACGGCAAAATAGTGGTCGAACTGGCCACGCCTCACATCGCTGTCGATATTCATAAAGCGCAAG
AAGGTCTGAGTATTGAATTGATGGACACGAGCGTTTCCGACGACAAGCTTTACGTATTGGATGTGAAAGACTTCGCTACC
AATGTGTCGTCGATAGAGGTGTTTCGTAAAGAGGATCGTGTCATTTTGCTCACCAGTGTCGATGGTGATTATGGCTACGA
CTACCATTTGACGGGCAACTATCTCGAATTGGTCGTCACTCAGCCCAAGGTAGAAACGAAGCTTCAAGAGAAAAGTATTC
TTGAAAAAAGTGGCAAAAAAATATCGATTAATTTTCAAGATATCCCAGTTCGTAACGTGCTTCAGCTTATCGCGGACTAC
AACGACTTTAACTTAGTCGTATCAGATTCGGTTCAAGGCAATTTAACGCTTCGTCTTGATGGTGTGCCTTGGCAACAAGT
GCTGGATATTATCCTTCAGGTGAAAGGCTTAGATAAGCGTATCGACGGCAATGTGGTTCTGGTTGCACCAAAGGCCGAGC
TAGATTTGCGTGAACAGCAGATGCTGGAAAAGTCGCGCCTAGAAGAAGAGTTGGGTGATTTGAGCTCAGAGATTATCAAG
ATTAAGTTTGCCAAAGCTTCGGAAATTGCAGAAATGATCGGCGGGCAGGGAGCCGCCAGTATGCTCTCTGAGCGCGGCTC
TTTAACTGTGGATGAACGCACTAATGCGCTTTTGATTCGTGATTTGAAAGAGAATATTGACGTTATTCGCGACATTATCG
ACACCCTCGACATTCCTGTAAAACAGGTTCAAATCGAAGCGCGCATTGTTAGTGTCAACGAAGGTAACTTAGATGAGCTA
GGTGTCCGTTGGGGCTTTACTTCTACGAACGGAAACAACACTGTGGGCGGCTCGATTGAGAGTAACCTGTTCCAAGCAGG
GTTGCTTGAAGGGGACAACTTGCCAGTGGATGACTTCTTAAATGTAAACCTAGGTGCCACGTCAGCGACTGCGTCTTCGA
TAGCGTTTCAAGTGGCAAAATTAGGCTCAGATCTGTTGTTAGACTTGGAGCTCTCAGCCTTGCAACGTGAGTCAAAAGCT
GAAGTGATCTCAAGTCCGCGCCTGATTACCACAAACAAAAAGCCTGCCTACATTGAACAGGGTACAGAAATTCCCTATTT
GGAGTCTTCGTCAAGTGGTGCGACTACGGTGACATTTAAGAAAGCGGTACTCAGTTTAAAAGTTACGCCGCAGATCACCC
CTGACAACCGTCTGGTTCTGGATTTAAATGTTACTCAAGATAGGCCTGGTGACGTAGTCAAAACCGGAACGGGTGAAGCG
GTGGCGATCAATACCCAGCGCATTGGTACCCAAGTGTTGGTAGAGAATGGCGAGACGGTCGTACTAGGGGGCATTTTCCA
GCACAGTGTTACCGATACGGTGGACAAGGTGCCTCTGCTTGGTGATTTACCGCTTTTGGGTGCGTTGTTTAGACGTTCTT
ATCAACAAATGGGCAAAAGTGAGCTGCTTATTTTTGTTACGCCGAAAGTCGTGATTCAGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(163-211)

Bacterial type II/III secretion system short domain (Secretin_N)

(237-302)

Bacterial type II and III secretion system protein (Secretin)

(393-551)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

75.045

100

0.75
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

75.045

100

0.75
  pilQ Vibrio campbellii strain DS40M4

76.243

98.192

0.749
  comQ Acinetobacter baylyi ADP1

40.104

100

0.418
  pilQ Pseudomonas aeruginosa PAK

40.526

100

0.418
  pilQ Legionella pneumophila strain ERS1305867

41.241

99.096

0.409
  pilQ Legionella pneumophila strain Lp02

41.241

99.096

0.409
  pilQ Acinetobacter baumannii strain A118

38.783

100

0.403
  pilQ Acinetobacter baumannii D1279779

38.783

100

0.403

Multiple sequence alignment