Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   U9J37_RS10195 Genome accession   NZ_OY808997
Coordinates   2235244..2235831 (-) Length   195 a.a.
NCBI ID   WP_005474158.1    Uniprot ID   -
Organism   Vibrio sp. 16 isolate VDT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2230244..2240831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9J37_RS10170 (VDT1_2076) aroB 2230628..2231716 (-) 1089 WP_005474138.1 3-dehydroquinate synthase -
  U9J37_RS10175 (VDT1_2077) aroK 2231742..2232260 (-) 519 WP_005474150.1 shikimate kinase AroK -
  U9J37_RS10180 (VDT1_2078) pilQ 2232437..2234098 (-) 1662 WP_005474140.1 type IV pilus secretin PilQ Machinery gene
  U9J37_RS10185 (VDT1_2079) pilP 2234159..2234671 (-) 513 WP_005474229.1 pilus assembly protein PilP Machinery gene
  U9J37_RS10190 (VDT1_2080) pilO 2234661..2235251 (-) 591 WP_005474200.1 type 4a pilus biogenesis protein PilO Machinery gene
  U9J37_RS10195 (VDT1_2081) pilN 2235244..2235831 (-) 588 WP_005474158.1 PilN domain-containing protein Machinery gene
  U9J37_RS10200 (VDT1_2082) pilM 2235821..2236816 (-) 996 WP_005474188.1 type IV pilus assembly protein PilM Machinery gene
  U9J37_RS10205 (VDT1_2083) - 2236955..2239498 (+) 2544 WP_043887187.1 penicillin-binding protein 1A -
  U9J37_RS10210 (VDT1_2084) oxyR 2239613..2240512 (-) 900 WP_005474154.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 195 a.a.        Molecular weight: 22181.34 Da        Isoelectric Point: 10.2757

>NTDB_id=1161982 U9J37_RS10195 WP_005474158.1 2235244..2235831(-) (pilN) [Vibrio sp. 16 isolate VDT1]
MLHSVNLIPWRDQLRDRHKHRFVGLLLIGVLVAVAAQWGAASYLQRQTQAQQSRLNYLNAYISQLDKQIDALKEVEQEHK
ALLTRLAVVEKLQNQRNKTTDFMNTLPQLIPEGVYVDKVKMNGLQVRVSGISDTTSRLATMLDNLESSTHIRNVEMHSIV
HDTPRFNKKFQSFNVSFSFVASAPNKQNVEVSSNG

Nucleotide


Download         Length: 588 bp        

>NTDB_id=1161982 U9J37_RS10195 WP_005474158.1 2235244..2235831(-) (pilN) [Vibrio sp. 16 isolate VDT1]
ATGCTGCATAGTGTCAATTTAATTCCATGGCGTGATCAACTACGCGACCGTCACAAACACCGATTCGTTGGCTTGCTTTT
AATTGGCGTCCTTGTTGCCGTAGCCGCTCAGTGGGGGGCGGCAAGCTACCTTCAACGCCAAACCCAAGCACAGCAGTCTC
GCTTGAATTACCTCAACGCCTACATTTCCCAACTTGATAAGCAGATTGATGCCTTAAAAGAAGTAGAGCAGGAGCATAAG
GCGCTGTTAACGCGACTTGCAGTGGTCGAGAAGCTACAAAATCAGCGTAATAAAACCACTGATTTTATGAATACATTGCC
GCAACTCATCCCTGAAGGTGTCTATGTCGACAAGGTGAAAATGAATGGTTTGCAGGTGCGTGTATCAGGGATCAGCGATA
CCACTTCTCGTTTGGCAACCATGCTCGATAACTTAGAGAGCTCCACCCACATTCGAAACGTAGAGATGCACTCCATCGTG
CATGACACCCCGCGGTTCAATAAGAAATTTCAAAGCTTTAATGTCTCTTTCTCCTTTGTTGCTAGTGCACCAAATAAACA
GAATGTAGAGGTAAGCAGCAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

60.428

95.897

0.579
  pilN Vibrio campbellii strain DS40M4

57.542

91.795

0.528

Multiple sequence alignment