Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   U9J37_RS10200 Genome accession   NZ_OY808997
Coordinates   2235821..2236816 (-) Length   331 a.a.
NCBI ID   WP_005474188.1    Uniprot ID   -
Organism   Vibrio sp. 16 isolate VDT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2230821..2241816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9J37_RS10175 (VDT1_2077) aroK 2231742..2232260 (-) 519 WP_005474150.1 shikimate kinase AroK -
  U9J37_RS10180 (VDT1_2078) pilQ 2232437..2234098 (-) 1662 WP_005474140.1 type IV pilus secretin PilQ Machinery gene
  U9J37_RS10185 (VDT1_2079) pilP 2234159..2234671 (-) 513 WP_005474229.1 pilus assembly protein PilP Machinery gene
  U9J37_RS10190 (VDT1_2080) pilO 2234661..2235251 (-) 591 WP_005474200.1 type 4a pilus biogenesis protein PilO Machinery gene
  U9J37_RS10195 (VDT1_2081) pilN 2235244..2235831 (-) 588 WP_005474158.1 PilN domain-containing protein Machinery gene
  U9J37_RS10200 (VDT1_2082) pilM 2235821..2236816 (-) 996 WP_005474188.1 type IV pilus assembly protein PilM Machinery gene
  U9J37_RS10205 (VDT1_2083) - 2236955..2239498 (+) 2544 WP_043887187.1 penicillin-binding protein 1A -
  U9J37_RS10210 (VDT1_2084) oxyR 2239613..2240512 (-) 900 WP_005474154.1 DNA-binding transcriptional regulator OxyR -
  U9J37_RS10215 (VDT1_2085) - 2240681..2241409 (+) 729 WP_005474181.1 redoxin family protein -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 36775.19 Da        Isoelectric Point: 6.8234

>NTDB_id=1161983 U9J37_RS10200 WP_005474188.1 2235821..2236816(-) (pilM) [Vibrio sp. 16 isolate VDT1]
MGKSLVIGVDIGHHSIKAVALKPSKGTFTLVSYQELPIEADIFTDNHMLNYQKIVKKLKELRKSLPLFSRKVALAIPDNA
VISKVLQIDSALDAQEREYAIFQAFAHQSPFPIEELNLDFVEVSRASASQALASYQVYATKRDVAESRVQAATKAGYQPV
LLDVQTHSLVHIWQMASRMQHKFDWLLVDVGLSQTSVCMDLQDKAPFCKEIALGTQLIENDHQLEHLVHGSTERFIFALV
DKLQRNIQMLTSVHGVSVQGIWLAGGGAVTPMLAEEIARRLNTECELFNPLALFERQNNKKSASYSTGHAYATAAGLALR
GLDWLEQDHAA

Nucleotide


Download         Length: 996 bp        

>NTDB_id=1161983 U9J37_RS10200 WP_005474188.1 2235821..2236816(-) (pilM) [Vibrio sp. 16 isolate VDT1]
ATGGGTAAATCTCTAGTCATCGGCGTTGATATTGGCCACCACAGTATAAAAGCGGTAGCTCTCAAGCCCTCAAAAGGCAC
GTTTACACTCGTGAGTTACCAAGAGTTGCCAATCGAAGCCGACATTTTCACTGATAACCACATGTTAAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGGAAGTCCCTACCGCTATTTAGTCGCAAAGTTGCGTTGGCAATACCAGACAATGCT
GTAATAAGTAAGGTTTTACAGATAGATAGCGCATTAGACGCGCAGGAAAGAGAGTACGCGATTTTCCAAGCGTTTGCTCA
TCAGTCGCCATTTCCTATCGAAGAGCTCAATCTTGACTTCGTTGAAGTGAGCCGAGCGAGTGCATCACAAGCTTTGGCCA
GCTATCAAGTGTATGCCACCAAACGTGATGTCGCTGAAAGTCGAGTTCAGGCCGCGACAAAAGCTGGCTATCAACCCGTA
TTACTCGATGTGCAAACGCACAGCCTGGTCCATATTTGGCAAATGGCATCAAGAATGCAGCACAAGTTTGATTGGTTGCT
GGTGGACGTTGGCTTGAGCCAAACCTCGGTGTGTATGGACTTGCAAGATAAGGCGCCTTTTTGCAAAGAGATTGCTTTAG
GGACGCAACTGATTGAAAACGATCATCAATTGGAGCACCTAGTCCACGGGAGTACAGAGCGTTTTATTTTTGCTCTGGTC
GATAAGTTGCAGCGCAACATTCAAATGCTCACTTCGGTTCATGGTGTTTCGGTGCAAGGAATCTGGCTTGCGGGCGGAGG
CGCCGTCACGCCGATGTTGGCAGAAGAAATCGCTAGGCGGCTGAACACTGAGTGTGAGCTGTTCAACCCGTTGGCGCTAT
TTGAGCGGCAAAACAACAAGAAATCCGCCAGTTATTCGACAGGCCATGCGTACGCAACAGCTGCAGGTTTGGCGTTAAGA
GGCCTGGATTGGTTGGAGCAAGATCATGCTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(235-321)

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

55.917

100

0.571
  pilM Vibrio campbellii strain DS40M4

44.776

100

0.453

Multiple sequence alignment