Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OM978_RS17015 Genome accession   NZ_OX352320
Coordinates   3593170..3594810 (-) Length   546 a.a.
NCBI ID   WP_264343468.1    Uniprot ID   -
Organism   Rheinheimera sp. MM224     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3588170..3599810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM978_RS16990 (JAMGFMIE_03418) - 3588262..3588528 (+) 267 WP_264343460.1 hypothetical protein -
  OM978_RS21540 (JAMGFMIE_03419) - 3588530..3588724 (+) 195 WP_413690726.1 DUF2970 domain-containing protein -
  OM978_RS16995 (JAMGFMIE_03420) - 3588643..3589542 (-) 900 WP_264343462.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  OM978_RS17000 (JAMGFMIE_03421) - 3589579..3591390 (-) 1812 WP_264343464.1 SPOR domain-containing protein -
  OM978_RS17005 (JAMGFMIE_03422) aroB 3591387..3592454 (-) 1068 WP_264343466.1 3-dehydroquinate synthase -
  OM978_RS17010 (JAMGFMIE_03423) aroK 3592493..3593011 (-) 519 WP_008900467.1 shikimate kinase AroK -
  OM978_RS17015 (JAMGFMIE_03424) pilQ 3593170..3594810 (-) 1641 WP_264343468.1 type IV pilus secretin PilQ Machinery gene
  OM978_RS17020 (JAMGFMIE_03425) - 3594892..3595437 (-) 546 WP_264343469.1 pilus assembly protein PilP -
  OM978_RS17025 (JAMGFMIE_03426) - 3595434..3596033 (-) 600 WP_233008318.1 type 4a pilus biogenesis protein PilO -
  OM978_RS17030 (JAMGFMIE_03427) - 3596030..3596641 (-) 612 WP_264343470.1 PilN domain-containing protein -
  OM978_RS17035 (JAMGFMIE_03428) - 3596629..3597708 (-) 1080 WP_264343471.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 546 a.a.        Molecular weight: 60262.11 Da        Isoelectric Point: 4.5231

>NTDB_id=1155317 OM978_RS17015 WP_264343468.1 3593170..3594810(-) (pilQ) [Rheinheimera sp. MM224]
MTAWAFAQQDSKDFINEIDSIDFRRGEQGEGKVLVFLQQNSAAVDVVEQNGQIQLDFHDTKISDELLYQLDVLDFATVVK
GIETFREGNKARLVIETLDAFTYSYQQIDEVFHLVVQKKSAEQTKTAAAGQYVGRAISLNFQDIPVRTVLQIIADYNGFN
LVTSDSVTGNITLRLDGTPWDQALDIVLKVRGLDKRMDGNILMIAPSDELAAREAKELQAQQQVEQLEPLMSEFLPINYA
KAADIAALLNNKEATLLSARGNVAVDDRTNTLLVKDTATTIENVRRLLRRLDVPVKQVLIESRMVTVKDNVRDEIGLRWG
FSDQQNDRAWSGNAPGANDLAGGTVPALNDRWNVNLPVANPAGSIAFHVTKFADGTLLDLELSALEQENKGEIIASPRIT
TANQKKARIEQGTEIPYLEASSSGATTVTFKKAVLSLEVTPQITPDNKIILDLEITQDTRGETVSTGNGPAQAIDTQRIQ
TQVLVDNGATVVLGGIYQQQMTNTTSKVPVFGDIPYLGNLFKRRNDFNEKTELLIFVTPKILTESL

Nucleotide


Download         Length: 1641 bp        

>NTDB_id=1155317 OM978_RS17015 WP_264343468.1 3593170..3594810(-) (pilQ) [Rheinheimera sp. MM224]
GTGACGGCTTGGGCTTTCGCACAGCAAGACAGCAAAGACTTTATTAATGAAATTGATTCGATAGATTTTCGCCGTGGTGA
ACAAGGCGAAGGCAAGGTACTGGTGTTCCTGCAACAAAACTCGGCTGCTGTGGATGTTGTTGAGCAAAATGGCCAAATTC
AGCTGGATTTCCATGACACTAAAATCAGCGATGAGCTGTTGTACCAACTGGATGTGCTGGATTTTGCCACTGTGGTCAAA
GGCATTGAAACTTTCCGCGAAGGCAACAAAGCCAGGTTAGTGATTGAAACCCTGGACGCTTTTACCTACAGCTACCAGCA
AATTGACGAGGTATTTCATCTGGTCGTGCAGAAAAAATCTGCAGAACAAACCAAAACAGCTGCAGCAGGCCAGTATGTCG
GTCGGGCTATTTCTCTAAATTTCCAGGATATTCCGGTCCGCACCGTGCTGCAGATCATTGCGGATTACAACGGCTTTAAT
CTGGTGACCAGCGACTCAGTGACAGGCAATATCACTTTACGGCTGGACGGCACACCCTGGGATCAGGCGCTGGATATAGT
GCTGAAAGTGCGTGGACTGGATAAACGTATGGACGGCAATATTCTGATGATAGCGCCGTCCGATGAGCTCGCCGCGCGTG
AAGCCAAAGAGCTGCAGGCTCAGCAACAGGTAGAGCAACTGGAGCCTTTAATGTCCGAGTTTCTACCTATTAATTACGCC
AAAGCGGCGGATATAGCTGCTTTACTCAACAATAAAGAAGCGACCTTATTATCTGCCCGTGGCAATGTGGCAGTGGATGA
CAGAACCAATACCTTATTGGTGAAAGATACAGCGACCACTATTGAAAACGTTCGGCGTTTATTAAGACGGCTGGATGTGC
CGGTGAAACAAGTGCTGATTGAATCCCGCATGGTGACAGTCAAAGACAATGTGCGGGACGAGATAGGTTTACGCTGGGGT
TTTAGTGATCAGCAAAATGACAGAGCCTGGTCTGGCAATGCACCTGGTGCCAATGATCTGGCAGGCGGCACAGTACCAGC
GCTGAATGACAGATGGAATGTGAATTTACCTGTAGCGAATCCTGCCGGTAGTATAGCTTTTCATGTAACTAAATTTGCCG
ATGGCACCTTGCTGGATTTGGAGTTGTCTGCACTCGAGCAGGAAAATAAAGGTGAAATTATTGCCAGCCCACGTATTACT
ACAGCCAATCAGAAAAAAGCCCGGATAGAGCAGGGCACTGAAATTCCTTACCTGGAAGCATCCAGCAGTGGAGCTACCAC
AGTCACCTTTAAAAAGGCGGTATTAAGCCTTGAAGTGACGCCCCAAATTACACCTGACAATAAAATCATTCTGGATTTAG
AGATCACGCAGGACACAAGGGGTGAAACTGTATCGACAGGCAATGGCCCTGCACAGGCGATAGACACTCAGCGCATCCAG
ACCCAGGTGCTGGTCGATAATGGCGCTACAGTAGTCTTGGGGGGCATCTATCAGCAGCAGATGACCAACACCACCTCAAA
AGTGCCGGTTTTTGGCGACATTCCGTATTTAGGGAATTTATTTAAACGCCGTAATGACTTTAATGAAAAAACCGAATTAC
TGATTTTTGTCACACCAAAAATTCTGACTGAAAGCTTGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(234-297)

Secretin and TonB N terminus short domain (STN)

(161-206)

Bacterial type II and III secretion system protein (Secretin)

(384-542)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

50.092

99.817

0.5
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

50.56

98.168

0.496
  pilQ Vibrio cholerae strain A1552

50.56

98.168

0.496
  pilQ Legionella pneumophila strain ERS1305867

47.048

99.267

0.467
  pilQ Legionella pneumophila strain Lp02

46.95

99.084

0.465
  comQ Acinetobacter baylyi ADP1

39.613

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.927

100

0.412
  pilQ Acinetobacter baumannii strain A118

38.927

100

0.412
  pilQ Pseudomonas aeruginosa PAK

40.143

100

0.41
  pilQ Neisseria meningitidis MC58

38.419

99.634

0.383
  pilQ Neisseria gonorrhoeae strain FA1090

38.278

100

0.383
  pilQ Neisseria meningitidis M1080

38.235

99.634

0.381

Multiple sequence alignment