Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HUW69_RS01460 Genome accession   NZ_LT992490
Coordinates   324080..325795 (+) Length   571 a.a.
NCBI ID   WP_032480167.1    Uniprot ID   -
Organism   Vibrio cholerae strain 4295STDY6534200     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 319080..330795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW69_RS01440 (SAMEA4374365_00297) pilM 321373..322389 (+) 1017 WP_029627685.1 type IV pilus assembly protein PilM Machinery gene
  HUW69_RS01445 (SAMEA4374365_00298) pilN 322379..322963 (+) 585 WP_029627684.1 PilN domain-containing protein Machinery gene
  HUW69_RS01450 (SAMEA4374365_00299) pilO 322956..323546 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  HUW69_RS01455 (SAMEA4374365_00300) pilP 323536..324054 (+) 519 WP_029627683.1 pilus assembly protein PilP Machinery gene
  HUW69_RS01460 (SAMEA4374365_00301) pilQ 324080..325795 (+) 1716 WP_032480167.1 type IV pilus secretin PilQ Machinery gene
  HUW69_RS01465 (SAMEA4374365_00302) aroK 325982..326506 (+) 525 WP_000818615.1 shikimate kinase AroK -
  HUW69_RS01470 (SAMEA4374365_00303) aroB 326534..327619 (+) 1086 WP_000439813.1 3-dehydroquinate synthase -
  HUW69_RS01475 (SAMEA4374365_00304) - 327673..329130 (+) 1458 WP_029627682.1 AAA family ATPase -
  HUW69_RS01480 (SAMEA4374365_00305) - 329200..330033 (+) 834 WP_029627681.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62315.47 Da        Isoelectric Point: 4.6627

>NTDB_id=1150261 HUW69_RS01460 WP_032480167.1 324080..325795(+) (pilQ) [Vibrio cholerae strain 4295STDY6534200]
MRNGLKTYVAQTWLTLWVGLALCASSTVFSAEFATTNQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLNIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKAPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGAISIDERTNSLLIRELPDNIAVIREIIESLDVPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSNSVGGSIESNLWQQGLLAGDKFPVDEFLNVNLASTSTNASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1150261 HUW69_RS01460 WP_032480167.1 324080..325795(+) (pilQ) [Vibrio cholerae strain 4295STDY6534200]
ATGAGAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTATGGGTCGGTTTGGCACTTTGTGCTTCTTCCAC
GGTGTTTAGTGCTGAATTCGCTACTACCAACCAGTTGGAAAATATTGACTTTCGAGTCAACAAAGAGAAAGCGGCGGTCT
TAATTGTCGAATTGGCCTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAACATTGAGCTGCTTAAA
ACCGATGTGGCGGATGACAAACTGTATCTGCTCGATGTGAAGGATTTTTCAACCCCTGTTGAGAGTGTGGAAGTATTCCG
TAAAGCGCCGAGCACACAACTTGTTGTGACTGTCGATGGTGAGTTTCAGCACGATTACACCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTTAAAGCTGATGAAAAGCCTAAGCCAAAGAGTGTCTTGGAAAAAGAAGGCAAGCTTATCTCG
ATTAACTTCCAAGATATTCCTGTACGTAACGTATTGCAGTTGATTGCCGATTATAACGGTTTCAACTTAGTTGTTTCTGA
CTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTTGATAAGCGCGTCGATGGCAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGATTTACGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCGGAAGAACTCGGCGATTTAAAATCAGAAATTATCAAGATTAACTTTGCCAAAGCATCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTCAACATGTTGTCTGAGCGAGGGGCGATCAGTATTGACGAACGGACTAACT
CACTGTTGATTCGAGAGCTACCCGATAACATCGCAGTGATCCGCGAGATCATCGAATCACTCGATGTTCCAGTCAAACAA
GTGCAAATTGAGGCACGAATCGTCACAGTGAAAGAAGGTAACTTGGAGGAGCTGGGGGTGCGCTGGGGCGTGATGTCGAC
CAATGGTAGCAACTCGGTCGGAGGAAGCATAGAAAGTAACTTATGGCAGCAAGGTTTATTGGCAGGTGATAAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCAACCTCTACTAACGCCTCTTCGATTGCATTCCAAGTGGCCAAATTAGGT
TCTGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCAAAGGCTGAAATCATCTCTAGCCCTCGTTTGAT
CACTACGAATAAACAGCCTGCCTATATTGAGCAAGGTACCGAGATCCCTTACCTAGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTGAGCTTGAAGGTGACGCCACAAATCACCCCAGATAACCGCTTGGTGCTGGATTTA
AGCGTGACCCAAGACCGACGGGGTGAAACGGTGAAAACGGGTACTGGAGAAGCTGTCTCCATCGATACTCAGCGCATTGG
TACTCAAGTACTGGTGAATAATGGAGAAACCGTGGTGCTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTACCACTGTTGGGGGACTTGCCTGTTTTAGGTGCGTTATTCAGACGAACCTATGAACAAATGGGCAAAAGTGAACTG
CTAATTTTTGTCACGCCGAAAGTCGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

97.723

100

0.977
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

97.723

100

0.977
  pilQ Vibrio campbellii strain DS40M4

75.13

100

0.762
  pilQ Legionella pneumophila strain ERS1305867

42.238

97.023

0.41
  pilQ Legionella pneumophila strain Lp02

42.238

97.023

0.41
  pilQ Acinetobacter baumannii D1279779

38.21

100

0.389
  pilQ Acinetobacter baumannii strain A118

38.21

100

0.389
  comQ Acinetobacter baylyi ADP1

38.128

100

0.385
  pilQ Pseudomonas aeruginosa PAK

38.809

97.023

0.377

Multiple sequence alignment