Detailed information NTG1150257

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HUW69_RS01440
• Genome accession: NZ_LT992490
• Coordinates: 321373..322389 (+)
• Length: 338 a.a.
• NCBI ID: WP_029627685.1
• Uniprot ID: -
• Organism: Vibrio cholerae strain 4295STDY6534200
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 316373..327389

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUW69_RS01425 (SAMEA4374365_00294)	-	316863..317594 (-)	732	WP_000495226.1	glutathione peroxidase	-
HUW69_RS01430 (SAMEA4374365_00295)	oxyR	317734..318627 (+)	894	WP_001025911.1	DNA-binding transcriptional regulator OxyR	-
HUW69_RS01435 (SAMEA4374365_00296)	-	318753..321230 (-)	2478	WP_029627686.1	penicillin-binding protein 1A	-
HUW69_RS01440 (SAMEA4374365_00297)	pilM	321373..322389 (+)	1017	WP_029627685.1	type IV pilus assembly protein PilM	Machinery gene
HUW69_RS01445 (SAMEA4374365_00298)	pilN	322379..322963 (+)	585	WP_029627684.1	PilN domain-containing protein	Machinery gene
HUW69_RS01450 (SAMEA4374365_00299)	pilO	322956..323546 (+)	591	WP_000157690.1	type 4a pilus biogenesis protein PilO	Machinery gene
HUW69_RS01455 (SAMEA4374365_00300)	pilP	323536..324054 (+)	519	WP_029627683.1	pilus assembly protein PilP	Machinery gene
HUW69_RS01460 (SAMEA4374365_00301)	pilQ	324080..325795 (+)	1716	WP_032480167.1	type IV pilus secretin PilQ	Machinery gene
HUW69_RS01465 (SAMEA4374365_00302)	aroK	325982..326506 (+)	525	WP_000818615.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37670.37 Da
• Isoelectric Point: 7.2623

>NTDB_id=1150257 HUW69_RS01440 WP_029627685.1 321373..322389(+) (pilM) [Vibrio cholerae strain 4295STDY6534200]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNNTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARGSTTTFQVYATKKDVVDSRLHAFKKAGFEP
VLMDVQVNSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVSSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGAQSLGGLWLSGGGATLSGLEEALYQRLSLPCEVLNPFSLFKNNVAKRKRSLVEGQRFST
ATGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=1150257 HUW69_RS01440 WP_029627685.1 321373..322389(+) (pilM) [Vibrio cholerae strain 4295STDY6534200]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCGGCAGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATAACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGGTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCACA
CCAATCTCCGTTTCCGGTGGAGGAACTGAGTCTCGATTTCGTCAAAGTGACGGAAAAGAGCCTTGCCCGCGGCAGCACCA
CGACTTTTCAAGTGTATGCCACCAAAAAAGACGTAGTCGACAGTCGTTTGCATGCCTTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTAAATAGTTTGTTACATTTATGGCAACTTGCTAGCCGTGCATATCGACGTCCCGACTGGAT
GTTGGTGGATATTGGTTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAGCTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCATGGTGCGCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGAAGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCTCACTGGTTGAGGGCCAGCGTTTTAGTACC
GCAACCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	97.041	100	0.97
pilM	Vibrio campbellii strain DS40M4	48.817	100	0.488