Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VCBK1071_RS02615 Genome accession   NZ_LT897797
Coordinates   531697..532125 (-) Length   142 a.a.
NCBI ID   WP_101410158.1    Uniprot ID   -
Organism   Vibrio cholerae isolate Vibrio cholerae str. BC1071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 526697..537125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCBK1071_RS02595 (VCBK1071_00503) coaE 527198..527806 (-) 609 WP_033928827.1 dephospho-CoA kinase -
  VCBK1071_RS02600 (VCBK1071_00504) pilD 527803..528678 (-) 876 WP_032478479.1 prepilin peptidase Machinery gene
  VCBK1071_RS02605 (VCBK1071_00505) pilC 528736..529962 (-) 1227 WP_101410156.1 type II secretion system F family protein Machinery gene
  VCBK1071_RS02610 (VCBK1071_00506) pilB 530005..531693 (-) 1689 WP_044127953.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VCBK1071_RS02615 (VCBK1071_00507) pilA 531697..532125 (-) 429 WP_101410158.1 pilin Machinery gene
  VCBK1071_RS02620 (VCBK1071_00508) nadC 532370..533260 (-) 891 WP_101410160.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VCBK1071_RS02625 (VCBK1071_00509) ampD 533558..534103 (+) 546 WP_101410162.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VCBK1071_RS02630 (VCBK1071_00510) fldB 534334..534852 (-) 519 WP_069503252.1 flavodoxin FldB -
  VCBK1071_RS02635 (VCBK1071_00511) xerD 534990..535898 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  VCBK1071_RS02640 (VCBK1071_00512) dsbC 535915..536667 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14677.79 Da        Isoelectric Point: 9.0103

>NTDB_id=1146656 VCBK1071_RS02615 WP_101410158.1 531697..532125(-) (pilA) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
MKAYKNKQQQGFTLIELMIVVAIIGVLAAIAVPQYQKYVAKSEAAAALASITGHRTNVETYVLENGSFPAIGDLAPPSAS
IGTIQYGAAQASGAGSIVFAFGTSGASPSVVNRNVTLARDENGSWKCTTTIDTKIAPKGCTP

Nucleotide


Download         Length: 429 bp        

>NTDB_id=1146656 VCBK1071_RS02615 WP_101410158.1 531697..532125(-) (pilA) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCGATTATTGGTGTTTT
GGCTGCCATTGCAGTGCCTCAGTACCAAAAATACGTCGCAAAAAGTGAGGCAGCAGCAGCACTGGCTTCGATCACTGGAC
ATAGAACAAACGTTGAAACATACGTATTGGAAAATGGTTCCTTCCCAGCAATAGGAGATTTAGCCCCGCCATCAGCAAGT
ATAGGCACTATTCAATATGGTGCCGCACAAGCATCTGGAGCTGGCAGCATAGTTTTTGCCTTCGGTACAAGTGGAGCAAG
CCCTAGTGTTGTAAATAGAAATGTTACTTTAGCTCGTGATGAAAATGGATCGTGGAAATGTACAACCACTATTGATACAA
AAATCGCGCCTAAAGGCTGTACACCATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

51.656

100

0.549
  pilA Vibrio cholerae strain A1552

51.656

100

0.549
  pilA Vibrio cholerae C6706

51.656

100

0.549
  pilA Pseudomonas aeruginosa PAK

43.624

100

0.458
  pilA Vibrio parahaemolyticus RIMD 2210633

43.056

100

0.437
  pilA Acinetobacter baumannii strain A118

42.754

97.183

0.415
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.972

100

0.415
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.007

99.296

0.387

Multiple sequence alignment