Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VCBK1071_RS02610 Genome accession   NZ_LT897797
Coordinates   530005..531693 (-) Length   562 a.a.
NCBI ID   WP_044127953.1    Uniprot ID   -
Organism   Vibrio cholerae isolate Vibrio cholerae str. BC1071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 525005..536693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCBK1071_RS02580 (VCBK1071_00501) yacG 525945..526142 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  VCBK1071_RS02590 (VCBK1071_00502) zapD 526426..527166 (-) 741 WP_076008577.1 cell division protein ZapD -
  VCBK1071_RS02595 (VCBK1071_00503) coaE 527198..527806 (-) 609 WP_033928827.1 dephospho-CoA kinase -
  VCBK1071_RS02600 (VCBK1071_00504) pilD 527803..528678 (-) 876 WP_032478479.1 prepilin peptidase Machinery gene
  VCBK1071_RS02605 (VCBK1071_00505) pilC 528736..529962 (-) 1227 WP_101410156.1 type II secretion system F family protein Machinery gene
  VCBK1071_RS02610 (VCBK1071_00506) pilB 530005..531693 (-) 1689 WP_044127953.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VCBK1071_RS02615 (VCBK1071_00507) pilA 531697..532125 (-) 429 WP_101410158.1 pilin Machinery gene
  VCBK1071_RS02620 (VCBK1071_00508) nadC 532370..533260 (-) 891 WP_101410160.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VCBK1071_RS02625 (VCBK1071_00509) ampD 533558..534103 (+) 546 WP_101410162.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VCBK1071_RS02630 (VCBK1071_00510) fldB 534334..534852 (-) 519 WP_069503252.1 flavodoxin FldB -
  VCBK1071_RS02635 (VCBK1071_00511) xerD 534990..535898 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  VCBK1071_RS02640 (VCBK1071_00512) dsbC 535915..536667 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62044.16 Da        Isoelectric Point: 6.0856

>NTDB_id=1146655 VCBK1071_RS02610 WP_044127953.1 530005..531693(-) (pilB) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAT
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=1146655 VCBK1071_RS02610 WP_044127953.1 530005..531693(-) (pilB) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
ATGCTCACCAACCTTGTTGCGATCTTGCGTCAGGCTGAGTTAATCAGCGCGACACAAGAACAAGCGGTGGTTACACAGGT
TAGCGCTTCGGGGACTTCAGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACCCCCAAGAGCTGACCGAGCAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCCCTGTGTCAACAGCTTGGGCTACGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTGGCGGTCTCTGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACTGGGCTGCAAGTTGAACTGGCGCTAGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCAGCCAACCAAGGGAAAGAAATCAGCCAAGATGAGCTCGCCACA
CTGGTCAAGGTCAGTGATGACGAGCTGCAATCCATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCTGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGCATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCTGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGTTTGCCGCAAGACGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTGATGACAGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTCAACACATCACAGATCAA
TATCTCCACCGCAGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACT
GCAGAAATCGCGGTAAAAGCAGCGCAAACCGGTCACTTGGTGCTTTCCACCCTGCACACCAACTCAGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTTGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACATTGCAAAATCGCGGTGCGCCCTTCTGCCCTATTGCAAAGCCAATTTGCGTTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTACAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.11

100

0.991
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Legionella pneumophila strain ERS1305867

52.838

90.925

0.48
  pilB Acinetobacter baylyi ADP1

50.385

92.527

0.466
  pilB Acinetobacter baumannii D1279779

50

92.883

0.464
  pilF Neisseria gonorrhoeae MS11

45.907

100

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

37.19

100

0.4
  pilF Thermus thermophilus HB27

39.372

96.263

0.379
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37

Multiple sequence alignment