Detailed information NTG1146653

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: VCBK1071_RS02600
• Genome accession: NZ_LT897797
• Coordinates: 527803..528678 (-)
• Length: 291 a.a.
• NCBI ID: WP_032478479.1
• Uniprot ID: -
• Organism: Vibrio cholerae isolate Vibrio cholerae str. BC1071
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 522803..533678

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VCBK1071_RS02575 (VCBK1071_00500)	parC	523611..525896 (+)	2286	WP_000102513.1	DNA topoisomerase IV subunit A	-
VCBK1071_RS02580 (VCBK1071_00501)	yacG	525945..526142 (-)	198	WP_000162865.1	DNA gyrase inhibitor YacG	-
VCBK1071_RS02590 (VCBK1071_00502)	zapD	526426..527166 (-)	741	WP_076008577.1	cell division protein ZapD	-
VCBK1071_RS02595 (VCBK1071_00503)	coaE	527198..527806 (-)	609	WP_033928827.1	dephospho-CoA kinase	-
VCBK1071_RS02600 (VCBK1071_00504)	pilD	527803..528678 (-)	876	WP_032478479.1	prepilin peptidase	Machinery gene
VCBK1071_RS02605 (VCBK1071_00505)	pilC	528736..529962 (-)	1227	WP_101410156.1	type II secretion system F family protein	Machinery gene
VCBK1071_RS02610 (VCBK1071_00506)	pilB	530005..531693 (-)	1689	WP_044127953.1	type IV-A pilus assembly ATPase PilB	Machinery gene
VCBK1071_RS02615 (VCBK1071_00507)	pilA	531697..532125 (-)	429	WP_101410158.1	pilin	Machinery gene
VCBK1071_RS02620 (VCBK1071_00508)	nadC	532370..533260 (-)	891	WP_101410160.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32465.75 Da
• Isoelectric Point: 7.1618

>NTDB_id=1146653 VCBK1071_RS02600 WP_032478479.1 527803..528678(-) (pilD) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSMVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide

• Length: 876 bp

>NTDB_id=1146653 VCBK1071_RS02600 WP_032478479.1 527803..528678(-) (pilD) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACCCTGTTTGGCTTGATTGTCGGCAGCTTTCTCAA
TGTGGTGATCTACCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGCCAAACACCGATTCGCGTCATT
GATAATATCCCGCTACTGAGTTGGCTGGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTAATTGCCACTCACTTTCCGTTCGGAGTGTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTATTGATTGCGGCGACCTTCATCGACTTCGATACCCTGTTGCTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCTGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGCTATCTCTCGCTGTGGTCGATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCACTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGATGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGGTGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCATTATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	99.656	100	0.997
pilD	Vibrio campbellii strain DS40M4	75	98.969	0.742
pilD	Acinetobacter nosocomialis M2	53.676	93.471	0.502
pilD	Acinetobacter baumannii D1279779	53.309	93.471	0.498
pilD	Neisseria gonorrhoeae MS11	44.792	98.969	0.443