Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   DQN33_RS00820 Genome accession   NZ_LS483408
Coordinates   131775..132716 (+) Length   313 a.a.
NCBI ID   WP_012657673.1    Uniprot ID   -
Organism   Streptococcus uberis strain NCTC4674     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 126775..137716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN33_RS00810 (NCTC4674_00165) rpoC 127578..131216 (+) 3639 WP_012657671.1 DNA-directed RNA polymerase subunit beta' -
  DQN33_RS00815 (NCTC4674_00166) - 131337..131702 (+) 366 WP_041817508.1 DUF1033 family protein -
  DQN33_RS00820 (NCTC4674_00167) comGA/cglA 131775..132716 (+) 942 WP_012657673.1 competence type IV pilus ATPase ComGA Machinery gene
  DQN33_RS00825 (NCTC4674_00168) comYB 132834..133682 (+) 849 WP_331813062.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQN33_RS00830 (NCTC4674_00169) comGC/cglC 133683..134006 (+) 324 WP_046388917.1 competence type IV pilus major pilin ComGC Machinery gene
  DQN33_RS00835 (NCTC4674_00170) comGD 133960..134397 (+) 438 WP_052733618.1 competence type IV pilus minor pilin ComGD -
  DQN33_RS00840 (NCTC4674_00171) comGE 134369..134668 (+) 300 WP_052733619.1 competence type IV pilus minor pilin ComGE -
  DQN33_RS00845 (NCTC4674_00172) comGF 134628..135083 (+) 456 WP_046388919.1 competence type IV pilus minor pilin ComGF -
  DQN33_RS00850 (NCTC4674_00173) comGG 135061..135420 (+) 360 WP_111672967.1 competence type IV pilus minor pilin ComGG -
  DQN33_RS00855 (NCTC4674_00174) comYH 135465..136421 (+) 957 WP_111672968.1 class I SAM-dependent methyltransferase Machinery gene
  DQN33_RS00860 (NCTC4674_00175) - 136477..137667 (+) 1191 WP_012657681.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35648.93 Da        Isoelectric Point: 6.7725

>NTDB_id=1140485 DQN33_RS00820 WP_012657673.1 131775..132716(+) (comGA/cglA) [Streptococcus uberis strain NCTC4674]
MIQSLGMQLIQEASLKQAQDIYLIPQKDSYQVFMRVGDSRQKIGDYEWKDATNLISHFKFVAGMNVGEKRRTQLGSCDYH
IDQQGLVSLRLSTVGDYRGHESLVIRLLATQESQLHYWFDSFERIEKAIHGRGLYLFAGPVGSGKTRLMYALMEKYMQDS
QIITIEDPVEIKQDAILQLQVNNSIGMTYDNLIKLSLRHRPDLVIIGEIRDTETAQAVIRASLTGAMVFSTIHAKSIPGV
FSRLIELGVKRDELLNCLNLIAYQRLIAGGGLIDFATEKFEEHTHNQWNQEVEQLLKQGYIDNEKAKLEKIKG

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1140485 DQN33_RS00820 WP_012657673.1 131775..132716(+) (comGA/cglA) [Streptococcus uberis strain NCTC4674]
ATGATACAAAGCTTAGGCATGCAATTGATTCAAGAAGCCAGTCTCAAACAAGCACAAGATATTTATCTCATACCTCAAAA
AGACTCTTATCAAGTTTTTATGAGAGTTGGAGATAGTCGGCAAAAAATAGGAGATTATGAGTGGAAGGATGCGACAAATC
TCATTAGTCATTTTAAATTTGTCGCAGGTATGAATGTTGGCGAAAAAAGAAGAACACAACTAGGGTCCTGTGATTATCAC
ATAGATCAGCAAGGCCTTGTCTCCTTGAGACTGTCAACAGTGGGTGACTATCGCGGGCATGAAAGTTTGGTTATCCGCCT
TTTAGCCACACAAGAGTCACAATTACATTATTGGTTTGACAGTTTTGAAAGGATTGAAAAAGCCATTCATGGTCGAGGTC
TTTATTTATTTGCTGGTCCAGTAGGTTCAGGTAAAACACGGTTGATGTACGCGTTAATGGAAAAATATATGCAGGACAGC
CAAATTATTACGATTGAAGATCCTGTTGAGATTAAGCAAGATGCTATTTTACAACTCCAAGTCAATAATAGTATTGGAAT
GACTTATGATAACCTGATTAAATTGTCTTTGAGGCATCGGCCTGATTTAGTGATTATAGGTGAAATACGTGATACAGAAA
CAGCACAGGCTGTTATTAGAGCAAGTTTGACGGGGGCCATGGTTTTCTCGACCATTCATGCAAAGAGTATTCCAGGAGTT
TTCTCTCGACTGATTGAACTCGGTGTGAAACGAGATGAATTGTTAAACTGTCTCAATTTGATAGCTTATCAACGGTTAAT
TGCAGGAGGAGGGCTAATTGATTTTGCCACAGAAAAATTCGAAGAGCATACCCATAATCAATGGAATCAAGAGGTTGAGC
AACTTCTTAAACAAGGATATATCGATAACGAAAAAGCCAAGTTGGAAAAAATTAAAGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

63.023

99.361

0.626
  comYA Streptococcus mutans UA140

62.5

99.681

0.623
  comYA Streptococcus mutans UA159

62.5

99.681

0.623
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.058

99.361

0.617
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.415

99.361

0.61
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.415

99.361

0.61
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.415

99.361

0.61
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.415

99.361

0.61
  comYA Streptococcus gordonii str. Challis substr. CH1

58.654

99.681

0.585
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508

Multiple sequence alignment