Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   DQN33_RS00825 Genome accession   NZ_LS483408
Coordinates   132834..133682 (+) Length   282 a.a.
NCBI ID   WP_331813062.1    Uniprot ID   -
Organism   Streptococcus uberis strain NCTC4674     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 127834..138682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN33_RS00815 (NCTC4674_00166) - 131337..131702 (+) 366 WP_041817508.1 DUF1033 family protein -
  DQN33_RS00820 (NCTC4674_00167) comGA/cglA 131775..132716 (+) 942 WP_012657673.1 competence type IV pilus ATPase ComGA Machinery gene
  DQN33_RS00825 (NCTC4674_00168) comYB 132834..133682 (+) 849 WP_331813062.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQN33_RS00830 (NCTC4674_00169) comGC/cglC 133683..134006 (+) 324 WP_046388917.1 competence type IV pilus major pilin ComGC Machinery gene
  DQN33_RS00835 (NCTC4674_00170) comGD 133960..134397 (+) 438 WP_052733618.1 competence type IV pilus minor pilin ComGD -
  DQN33_RS00840 (NCTC4674_00171) comGE 134369..134668 (+) 300 WP_052733619.1 competence type IV pilus minor pilin ComGE -
  DQN33_RS00845 (NCTC4674_00172) comGF 134628..135083 (+) 456 WP_046388919.1 competence type IV pilus minor pilin ComGF -
  DQN33_RS00850 (NCTC4674_00173) comGG 135061..135420 (+) 360 WP_111672967.1 competence type IV pilus minor pilin ComGG -
  DQN33_RS00855 (NCTC4674_00174) comYH 135465..136421 (+) 957 WP_111672968.1 class I SAM-dependent methyltransferase Machinery gene
  DQN33_RS00860 (NCTC4674_00175) - 136477..137667 (+) 1191 WP_012657681.1 acetate kinase -
  DQN33_RS00865 (NCTC4674_00176) - 137785..137988 (+) 204 WP_012657682.1 helix-turn-helix transcriptional regulator -
  DQN33_RS00870 - 137990..138442 (+) 453 WP_111672969.1 hypothetical protein -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 32522.89 Da        Isoelectric Point: 8.3916

>NTDB_id=1140486 DQN33_RS00825 WP_331813062.1 132834..133682(+) (comYB) [Streptococcus uberis strain NCTC4674]
MKVDLMNGRDISEMMAHLGFSDALVTQLSLAEVHGNTTNCLAKIEDYMAQSNRIRQKTIEVITYPMVLMTFLIVILMGLR
HYLLPQIGEENQLTHFLNLFPTVLLLTFFAMIAVVIFLRLCWRKKSQLKQLLVYSKIPLLSAVLQLYLTAYYAREWGNLI
AQGVELATILQLMQKEKSVLMREMGSTMEKALLEGKSFDQSVGIFPFFKRELSLMIEYGEIKGKLGQELDIYAQVSWENY
FRKLRQMTQWLQPLIFILVAIIIVMIYAAMLLPMYQTIGGEI

Nucleotide


Download         Length: 849 bp        

>NTDB_id=1140486 DQN33_RS00825 WP_331813062.1 132834..133682(+) (comYB) [Streptococcus uberis strain NCTC4674]
ATGAAAGTAGATTTAATGAATGGACGAGATATTTCGGAAATGATGGCCCATCTTGGATTCTCAGATGCATTGGTAACACA
GCTAAGTTTAGCTGAAGTCCATGGGAACACCACCAACTGTTTAGCAAAGATAGAAGATTATATGGCTCAATCCAATCGGA
TTAGACAAAAAACCATTGAAGTCATTACTTATCCTATGGTCTTGATGACCTTCTTAATCGTGATTTTGATGGGCTTACGC
CATTATTTACTTCCGCAAATAGGAGAGGAAAACCAGCTAACACATTTTCTAAATTTATTTCCAACAGTATTGCTTTTGAC
TTTCTTTGCTATGATTGCGGTAGTAATCTTCCTCAGACTCTGCTGGCGAAAAAAATCCCAGTTAAAACAATTATTAGTGT
ATAGTAAAATCCCTCTTTTATCAGCTGTTTTACAATTGTATCTAACTGCCTATTATGCCAGAGAGTGGGGCAATCTTATT
GCACAAGGTGTTGAACTAGCAACTATTTTGCAACTGATGCAAAAGGAAAAATCCGTATTGATGCGGGAAATGGGTTCAAC
AATGGAAAAAGCTTTGTTGGAAGGGAAATCTTTTGACCAGTCTGTGGGCATCTTCCCATTTTTTAAAAGGGAATTGAGCT
TAATGATTGAATATGGAGAAATCAAAGGAAAACTCGGTCAAGAGTTAGACATTTATGCACAAGTCAGTTGGGAAAACTAT
TTTAGAAAGTTACGACAGATGACTCAATGGTTACAACCCCTCATTTTCATACTTGTAGCCATTATAATCGTTATGATTTA
TGCGGCAATGTTATTGCCAATGTATCAAACCATCGGAGGTGAAATATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(3-85)

Type II secretion system (T2SS), protein F (T2SSF)

(153-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA159

54.676

98.582

0.539
  comYB Streptococcus mutans UA140

54.676

98.582

0.539
  comGB/cglB Streptococcus mitis NCTC 12261

48.746

98.936

0.482
  comGB Lactococcus lactis subsp. cremoris KW2

45.804

100

0.465
  comYB Streptococcus gordonii str. Challis substr. CH1

47.122

98.582

0.465
  comGB/cglB Streptococcus mitis SK321

47.122

98.582

0.465
  comGB/cglB Streptococcus pneumoniae Rx1

46.043

98.582

0.454
  comGB/cglB Streptococcus pneumoniae D39

46.043

98.582

0.454
  comGB/cglB Streptococcus pneumoniae R6

46.043

98.582

0.454
  comGB/cglB Streptococcus pneumoniae TIGR4

46.043

98.582

0.454

Multiple sequence alignment