Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   DQM57_RS08395 Genome accession   NZ_LS483369
Coordinates   1624167..1624643 (+) Length   158 a.a.
NCBI ID   WP_108044216.1    Uniprot ID   A0A7T3ETK6
Organism   Neisseria cinerea strain NCTC10294     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1619167..1629643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM57_RS08370 (NCTC10294_01593) - 1619344..1619829 (+) 486 WP_111727508.1 CinA family protein -
  DQM57_RS08375 (NCTC10294_01594) msrAB 1619914..1621482 (-) 1569 WP_111727509.1 bifunctional peptide-methionine (S)-S-oxide reductase MsrA/peptide-methionine (R)-S-oxide reductase MsrB -
  DQM57_RS08380 (NCTC10294_01595) pilA 1621628..1623052 (+) 1425 WP_111727510.1 signal recognition particle-docking protein FtsY Machinery gene
  DQM57_RS08385 (NCTC10294_01596) - 1623363..1623629 (+) 267 WP_019271767.1 DUF3079 domain-containing protein -
  DQM57_RS08390 (NCTC10294_01597) - 1623839..1624099 (+) 261 WP_111727511.1 hypothetical protein -
  DQM57_RS08395 (NCTC10294_01598) pilA2 1624167..1624643 (+) 477 WP_108044216.1 pilin Machinery gene
  DQM57_RS09980 (NCTC10294_01599) - 1624684..1625307 (+) 624 WP_269460473.1 pilin -
  DQM57_RS09985 - 1625304..1625426 (-) 123 WP_003678392.1 hypothetical protein -
  DQM57_RS08405 (NCTC10294_01600) yccS 1625446..1627596 (-) 2151 WP_111727512.1 YccS family putative transporter -
  DQM57_RS08410 (NCTC10294_01601) - 1627714..1628940 (-) 1227 WP_003678396.1 PilT/PilU family type 4a pilus ATPase -

Sequence

Protein


Download         Length: 158 a.a.        Molecular weight: 16316.76 Da        Isoelectric Point: 8.4686

>NTDB_id=1138965 DQM57_RS08395 WP_108044216.1 1624167..1624643(+) (pilA2) [Neisseria cinerea strain NCTC10294]
MKAIQKGFTLIELMIVIAILGILAVIAFPAYQDYTIRAKVSEGLNLAAPAKLAVAETAASLGGLDAVTPDNNGYTFPKTG
TKYVASIAIAKKGVITVTTKATGATHDPIFVLTPTQTNSESPVEWACTYQGDSEAKHVPANCRTKAAAAPAASAGNSH

Nucleotide


Download         Length: 477 bp        

>NTDB_id=1138965 DQM57_RS08395 WP_108044216.1 1624167..1624643(+) (pilA2) [Neisseria cinerea strain NCTC10294]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAGCTGATGATCGTTATCGCCATCCTGGGTATTCTGGCCGTTATCGC
ATTCCCTGCATACCAAGACTACACCATCCGCGCTAAAGTATCCGAAGGTCTGAACTTGGCTGCGCCTGCTAAATTGGCAG
TAGCGGAAACTGCCGCTTCTTTGGGTGGTTTGGATGCAGTTACGCCTGATAATAACGGTTATACTTTCCCTAAAACCGGT
ACTAAGTATGTAGCATCTATCGCGATTGCTAAAAAAGGTGTAATCACTGTAACCACTAAAGCTACCGGTGCTACTCACGA
TCCTATCTTTGTTTTGACTCCTACTCAAACTAATTCAGAGTCTCCTGTTGAATGGGCTTGTACATACCAAGGTGATAGCG
AGGCAAAACACGTTCCTGCAAACTGCCGAACTAAAGCTGCAGCTGCACCTGCTGCATCTGCTGGTAATTCACATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(37-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T3ETK6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

57.857

88.608

0.513
  pilA2 Legionella pneumophila strain ERS1305867

56.429

88.608

0.5
  pilA Ralstonia pseudosolanacearum GMI1000

42.353

100

0.456
  pilE Neisseria gonorrhoeae MS11

40.588

100

0.437
  comP Acinetobacter baylyi ADP1

45.033

95.57

0.43
  pilE Neisseria gonorrhoeae strain FA1090

39.881

100

0.424
  pilA Haemophilus influenzae Rd KW20

41.216

93.671

0.386
  pilA Haemophilus influenzae 86-028NP

40.136

93.038

0.373
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.278

91.139

0.367
  pilA Glaesserella parasuis strain SC1401

38.158

96.203

0.367

Multiple sequence alignment