Detailed information NTG1137695

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DQM73_RS03640
• Genome accession: NZ_LS483346
• Coordinates: 697680..698360 (+)
• Length: 226 a.a.
• NCBI ID: WP_032908211.1
• Uniprot ID: A0A2X3VED2
• Organism: Streptococcus sanguinis strain NCTC11085
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 692680..703360

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQM73_RS03620 (NCTC11085_00703)	-	692797..693543 (-)	747	WP_002912006.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
DQM73_RS03625 (NCTC11085_00704)	-	693674..696013 (-)	2340	WP_002934841.1	cation-translocating P-type ATPase	-
DQM73_RS03630 (NCTC11085_00705)	-	696171..696911 (+)	741	WP_002925501.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
DQM73_RS03635 (NCTC11085_00706)	-	696984..697532 (+)	549	WP_172455810.1	HXXEE domain-containing protein	-
DQM73_RS03640 (NCTC11085_00707)	comEA	697680..698360 (+)	681	WP_032908211.1	helix-hairpin-helix domain-containing protein	Machinery gene
DQM73_RS03645 (NCTC11085_00708)	comEC/celB	698344..700584 (+)	2241	WP_002925496.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DQM73_RS03650 (NCTC11085_00709)	-	700709..701845 (+)	1137	WP_002925494.1	DUF805 domain-containing protein	-
DQM73_RS03655 (NCTC11085_00710)	holA	702097..703143 (+)	1047	WP_032908210.1	DNA polymerase III subunit delta	-

Sequence

Protein

• Length: 226 a.a.
• Molecular weight: 23928.68 Da
• Isoelectric Point: 4.5935

>NTDB_id=1137695 DQM73_RS03640 WP_032908211.1 697680..698360(+) (comEA) [Streptococcus sanguinis strain NCTC11085]
MLEEIIEKLKEYKLFVGLAVVGAVLGGFFLIKGNHQPQNQVAALSQEITSSSSSADEESEKVVSKDKADEEESEQVTVDI
KGAVKNPGVYELRAGARVHEAIQKAGGLTADAEAKSINQAQKLMDEAVIYVAKIGEEGADVTQTGQHQAGVSDSAGASGG
KSDKVNLNTATEAELQTISGIGQKRASDIIAYRESNGSFKSVDDLKKVSGIGDKTLEKLKEYVTVD

Nucleotide

• Length: 681 bp

>NTDB_id=1137695 DQM73_RS03640 WP_032908211.1 697680..698360(+) (comEA) [Streptococcus sanguinis strain NCTC11085]
ATGCTTGAAGAAATCATTGAAAAGCTGAAAGAATACAAGCTCTTTGTTGGTCTAGCAGTAGTTGGCGCTGTGCTAGGTGG
CTTTTTCCTCATCAAAGGAAATCATCAGCCGCAAAATCAGGTAGCCGCCTTGAGTCAAGAAATAACTTCCTCATCCAGCT
CAGCTGATGAAGAGAGTGAAAAGGTCGTCTCTAAGGACAAGGCGGACGAAGAGGAGAGTGAGCAAGTCACTGTGGATATT
AAAGGCGCAGTTAAGAATCCTGGTGTCTATGAGTTGAGAGCAGGAGCGAGAGTCCATGAAGCCATCCAAAAAGCTGGCGG
ATTGACAGCAGACGCCGAGGCTAAGTCCATCAATCAGGCGCAAAAATTAATGGATGAAGCAGTAATCTATGTGGCAAAGA
TTGGTGAAGAAGGAGCCGATGTCACACAGACTGGACAGCATCAAGCAGGAGTTTCTGATTCTGCGGGTGCCAGTGGTGGA
AAGTCAGACAAGGTCAATCTAAATACGGCGACAGAGGCTGAACTGCAGACTATTTCAGGCATCGGACAGAAGCGAGCTTC
TGACATCATCGCCTACCGCGAAAGCAATGGAAGTTTCAAGTCAGTAGATGATTTGAAAAAGGTATCAGGAATTGGGGATA
AGACCTTAGAAAAGCTGAAAGAATATGTTACAGTGGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (78-131)

• Helix-hairpin-helix motif (HHH_3) (162-224)

3D structure

• AlphaFold DB: A0A2X3VED2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	50	100	0.513
comEA/celA/cilE	Streptococcus mitis SK321	49.569	100	0.509
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	49.569	100	0.509
comEA/celA/cilE	Streptococcus pneumoniae R6	49.138	100	0.504
comEA/celA/cilE	Streptococcus pneumoniae Rx1	49.138	100	0.504
comEA/celA/cilE	Streptococcus pneumoniae D39	49.138	100	0.504
comEA/celA/cilE	Streptococcus mitis NCTC 12261	49.778	99.558	0.496
comEA	Bacillus subtilis subsp. subtilis str. 168	42.986	97.788	0.42
comEA	Lactococcus lactis subsp. cremoris KW2	41.15	100	0.412
comEA	Latilactobacillus sakei subsp. sakei 23K	34.286	100	0.372
comEA	Staphylococcus aureus MW2	36.771	98.673	0.363