Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   DQL25_RS00610 Genome accession   NZ_LS483330
Coordinates   96035..96973 (+) Length   312 a.a.
NCBI ID   WP_023612548.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8328     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 91035..101973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL25_RS00600 (NCTC8328_00120) rpoC 91802..95425 (+) 3624 WP_111703091.1 DNA-directed RNA polymerase subunit beta' -
  DQL25_RS00605 (NCTC8328_00121) - 95577..95942 (+) 366 WP_002986560.1 DUF1033 family protein -
  DQL25_RS00610 (NCTC8328_00122) comYA 96035..96973 (+) 939 WP_023612548.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL25_RS00615 (NCTC8328_00123) comYB 96909..97943 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL25_RS00620 (NCTC8328_00124) comYC 97945..98271 (+) 327 WP_032465607.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL25_RS00625 (NCTC8328_00125) comGD 98246..98674 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DQL25_RS00630 (NCTC8328_00126) comGE 98631..98915 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  DQL25_RS00635 (NCTC8328_00127) comYF 98908..99342 (+) 435 WP_111703092.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL25_RS00640 (NCTC8328_00128) comGG 99326..99652 (+) 327 WP_002992739.1 competence type IV pilus minor pilin ComGG -
  DQL25_RS00645 (NCTC8328_00129) comYH 99750..100703 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  DQL25_RS00650 (NCTC8328_00130) - 100762..101958 (+) 1197 WP_023612537.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35045.27 Da        Isoelectric Point: 8.5799

>NTDB_id=1136831 DQL25_RS00610 WP_023612548.1 96035..96973(+) (comYA) [Streptococcus pyogenes strain NCTC8328]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLVSHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1136831 DQL25_RS00610 WP_023612548.1 96035..96973(+) (comYA) [Streptococcus pyogenes strain NCTC8328]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTGTTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTCAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAGGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCATCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

61.415

99.679

0.612
  comYA Streptococcus mutans UA140

61.415

99.679

0.612
  comYA Streptococcus gordonii str. Challis substr. CH1

60.45

99.679

0.603
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.359

0.599
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.164

99.679

0.59
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.521

99.679

0.583
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.521

99.679

0.583
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.521

99.679

0.583
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.521

99.679

0.583
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

39.655

92.949

0.369

Multiple sequence alignment