Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   DQL25_RS00635 Genome accession   NZ_LS483330
Coordinates   98908..99342 (+) Length   144 a.a.
NCBI ID   WP_111703092.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8328     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 93908..104342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL25_RS00605 (NCTC8328_00121) - 95577..95942 (+) 366 WP_002986560.1 DUF1033 family protein -
  DQL25_RS00610 (NCTC8328_00122) comYA 96035..96973 (+) 939 WP_023612548.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL25_RS00615 (NCTC8328_00123) comYB 96909..97943 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL25_RS00620 (NCTC8328_00124) comYC 97945..98271 (+) 327 WP_032465607.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL25_RS00625 (NCTC8328_00125) comGD 98246..98674 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DQL25_RS00630 (NCTC8328_00126) comGE 98631..98915 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  DQL25_RS00635 (NCTC8328_00127) comYF 98908..99342 (+) 435 WP_111703092.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL25_RS00640 (NCTC8328_00128) comGG 99326..99652 (+) 327 WP_002992739.1 competence type IV pilus minor pilin ComGG -
  DQL25_RS00645 (NCTC8328_00129) comYH 99750..100703 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  DQL25_RS00650 (NCTC8328_00130) - 100762..101958 (+) 1197 WP_023612537.1 acetate kinase -
  DQL25_RS00655 - 102145..102453 (+) 309 WP_111703093.1 hypothetical protein -
  DQL25_RS00660 (NCTC8328_00131) proC 102536..103306 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16658.27 Da        Isoelectric Point: 10.2526

>NTDB_id=1136834 DQL25_RS00635 WP_111703092.1 98908..99342(+) (comYF) [Streptococcus pyogenes strain NCTC8328]
MSKQLSNIKAFTFLEALIALLVISGSLLVYQGLTRALLKHSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1136834 DQL25_RS00635 WP_111703092.1 98908..99342(+) (comYF) [Streptococcus pyogenes strain NCTC8328]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCTTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCGAGCCCTCCTTAAACATAGCCATTATCTAGCCCGTCATGATCAAGATAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

43.284

93.056

0.403
  comGF/cglF Streptococcus pneumoniae Rx1

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae D39

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae R6

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae TIGR4

42.105

92.361

0.389
  comGF/cglF Streptococcus mitis SK321

41.353

92.361

0.382

Multiple sequence alignment