Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   DQL25_RS00645 Genome accession   NZ_LS483330
Coordinates   99750..100703 (+) Length   317 a.a.
NCBI ID   WP_002987790.1    Uniprot ID   A0A5S4TCX5
Organism   Streptococcus pyogenes strain NCTC8328     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 94750..105703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL25_RS00605 (NCTC8328_00121) - 95577..95942 (+) 366 WP_002986560.1 DUF1033 family protein -
  DQL25_RS00610 (NCTC8328_00122) comYA 96035..96973 (+) 939 WP_023612548.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL25_RS00615 (NCTC8328_00123) comYB 96909..97943 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL25_RS00620 (NCTC8328_00124) comYC 97945..98271 (+) 327 WP_032465607.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL25_RS00625 (NCTC8328_00125) comGD 98246..98674 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DQL25_RS00630 (NCTC8328_00126) comGE 98631..98915 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  DQL25_RS00635 (NCTC8328_00127) comYF 98908..99342 (+) 435 WP_111703092.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL25_RS00640 (NCTC8328_00128) comGG 99326..99652 (+) 327 WP_002992739.1 competence type IV pilus minor pilin ComGG -
  DQL25_RS00645 (NCTC8328_00129) comYH 99750..100703 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  DQL25_RS00650 (NCTC8328_00130) - 100762..101958 (+) 1197 WP_023612537.1 acetate kinase -
  DQL25_RS00655 - 102145..102453 (+) 309 WP_111703093.1 hypothetical protein -
  DQL25_RS00660 (NCTC8328_00131) proC 102536..103306 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -
  DQL25_RS00665 (NCTC8328_00132) pepA 103354..104421 (-) 1068 WP_111703094.1 glutamyl aminopeptidase -
  DQL25_RS00675 (NCTC8328_00133) - 104886..105170 (+) 285 WP_002992749.1 DUF4651 domain-containing protein -
  DQL25_RS00680 (NCTC8328_00134) - 105167..105484 (+) 318 WP_002992751.1 thioredoxin family protein -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 35985.08 Da        Isoelectric Point: 4.7205

>NTDB_id=1136835 DQL25_RS00645 WP_002987790.1 99750..100703(+) (comYH) [Streptococcus pyogenes strain NCTC8328]
MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEGASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQT
EQLQANHQFTPDAIGFILLYLLEQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSLKYLKKDGFAIFLAPVNLLTSPQ
SQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIVLQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN

Nucleotide


Download         Length: 954 bp        

>NTDB_id=1136835 DQL25_RS00645 WP_002987790.1 99750..100703(+) (comYH) [Streptococcus pyogenes strain NCTC8328]
ATGACTTTTGAAAAAATTGAAGAAGCTTATCAGCTGCTCTTAGAGAACTGTCAACTAATTGAAAATGATCTAAAAACACA
TATATACGATGCCATTGTTGAACAAAACTCCTTTTATTTAGGGGCTGAGGGAGCTAGTCCTCAAGTTGCTCAAAATAGTG
ATAAACTGAAAGCCTTGTGCCTGACAAAAGAAGAATGGCGTAAGGCCTACCAGTTTCTTTTTATTAAGGCAGCTCAGACT
GAGCAACTCCAAGCCAACCATCAGTTCACACCAGATGCTATTGGCTTCATTCTGCTGTATCTTTTGGAACAATTGAGTGA
TAAAGATAGCTTAGAGGTACTTGAGATTGGAAGTGGAACAGGGAACCTAGCCCAAACCCTTCTCAACAACACGAGCAAGA
GCCTTGATTATGTAGGGATTGAACTTGATGATCTCTTGATTGATCTGTCAGCCAGTATTGCTGAAATAATGGATTCTTCA
GCTCATTTTATTCAAGAAGATGCGGTAAGGCCTCAATTACTAAAAGAAAGTGACATTGTCATCAGTGACTTACCAGTTGG
TTATTATCCTAACGATGATATTGCCAAACGGTACAAGGTGGCTAGTTCAGATAAGCATACCTATGCCCATCATTTATTAA
TGGAACAGTCTTTAAAATACTTGAAAAAAGACGGTTTTGCGATTTTTCTGGCACCAGTCAATTTATTGACGAGCCCTCAG
AGCCAGTTATTGAAACAGTGGTTAAAAGATTATGCTCAGGTGGTGGCCTTGATTACACTACCAGATTCTATTTTTGGTCA
TCCCTCAAATGCCAAGTCCATTATTGTCTTACAAAAACAAACAGACCACCCAATGGAAACCTTTGTCTATCCAATTCGGG
ATTTGAAGCTTGCAGAGAATATTCATGATTTTATGGAAAATTTCAAAAAATGGAAACTGAGTAATGTCAATTAA

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(68-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TCX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA159

66.139

99.685

0.659
  comYH Streptococcus mutans UA140

65.823

99.685

0.656

Multiple sequence alignment