Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ACLVWI_RS16545 Genome accession   NZ_LR962897
Coordinates   3840181..3840609 (-) Length   142 a.a.
NCBI ID   WP_230433800.1    Uniprot ID   -
Organism   Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1586     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3840949..3849762 3840181..3840609 flank 340


Gene organization within MGE regions


Location: 3840181..3849762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLVWI_RS16545 (X12_003301) pilA/pilAI 3840181..3840609 (-) 429 WP_230433800.1 pilin Machinery gene
  ACLVWI_RS16550 (X12_003302) pilC 3840949..3842205 (+) 1257 WP_411862068.1 type II secretion system F family protein Machinery gene
  ACLVWI_RS16555 (X12_003303) - 3842212..3843075 (+) 864 WP_411862069.1 prepilin peptidase -
  ACLVWI_RS16560 (X12_003304) coaE 3843089..3843694 (+) 606 WP_411862070.1 dephospho-CoA kinase -
  ACLVWI_RS16565 - 3844120..3844566 (+) 447 Protein_3246 DUF6531 domain-containing protein -
  ACLVWI_RS16570 (X12_003305) - 3844747..3848328 (+) 3582 WP_411863117.1 RHS repeat-associated core domain-containing protein -
  ACLVWI_RS16575 (X12_003306) - 3848671..3848994 (+) 324 WP_104646208.1 hypothetical protein -
  ACLVWI_RS16580 (X12_003307) - 3849367..3849762 (+) 396 WP_411862071.1 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14412.69 Da        Isoelectric Point: 8.4970

>NTDB_id=1134953 ACLVWI_RS16545 WP_230433800.1 3840181..3840609(-) (pilA/pilAI) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1586]
MKKQNGFTLIELMIVIAIIAILAAIALPMYQDYVAKSQVAAGLAEITPGKVNAETRIAEGKAKTAVAADLGLATSGRCTK
VDVAVDPSGTSSIACTLAGNAQVATKTITWSRTADDATTGAAGVWSCASTVAQKLKPATCQG

Nucleotide


Download         Length: 429 bp        

>NTDB_id=1134953 ACLVWI_RS16545 WP_230433800.1 3840181..3840609(-) (pilA/pilAI) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1586]
ATGAAGAAGCAAAACGGTTTTACGCTGATCGAATTGATGATCGTCATTGCGATTATCGCAATTTTGGCTGCAATTGCCCT
GCCGATGTATCAGGACTACGTAGCGAAGTCGCAGGTTGCGGCCGGACTTGCAGAAATCACCCCGGGCAAGGTCAACGCTG
AGACGCGTATCGCCGAAGGGAAAGCGAAGACTGCGGTAGCTGCAGATCTTGGGTTGGCGACCAGCGGTCGTTGCACGAAG
GTCGATGTCGCTGTTGATCCTTCTGGCACTTCAAGTATCGCTTGCACCCTTGCTGGTAACGCACAGGTTGCCACTAAGAC
CATTACTTGGTCGCGCACCGCTGACGACGCCACAACTGGAGCCGCTGGCGTTTGGTCTTGCGCTTCCACTGTCGCCCAGA
AGCTAAAGCCAGCTACCTGCCAAGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

51.429

98.592

0.507
  pilA Acinetobacter baumannii strain A118

44.444

100

0.451
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.958

100

0.43
  pilA Pseudomonas aeruginosa PAK

38.312

100

0.415
  pilA Vibrio cholerae C6706

38.889

100

0.394
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.889

100

0.394
  pilA Vibrio cholerae strain A1552

38.889

100

0.394
  pilA Vibrio parahaemolyticus RIMD 2210633

39.552

94.366

0.373
  comP Acinetobacter baylyi ADP1

35.333

100

0.373
  pilA Ralstonia pseudosolanacearum GMI1000

33.333

100

0.373

Multiple sequence alignment