Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   H1X04_RS08525 Genome accession   NZ_LR822043
Coordinates   1649217..1650158 (-) Length   313 a.a.
NCBI ID   WP_011226629.1    Uniprot ID   -
Organism   Streptococcus thermophilus isolate STH_CIRM_2101     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1644217..1655158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1X04_RS08485 (STHERMO_1885) - 1644293..1645486 (-) 1194 WP_022096898.1 acetate kinase -
  H1X04_RS08490 (STHERMO_1886) comYH 1645542..1646498 (-) 957 WP_180480160.1 class I SAM-dependent methyltransferase Machinery gene
  H1X04_RS08495 (STHERMO_1887) comGG 1646543..1646860 (-) 318 WP_011226623.1 competence type IV pilus minor pilin ComGG -
  H1X04_RS08500 (STHERMO_1888) comYF 1646838..1647275 (-) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  H1X04_RS08505 (STHERMO_1889) comGE 1647259..1647552 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  H1X04_RS08510 (STHERMO_1890) comYD 1647524..1647952 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  H1X04_RS08515 (STHERMO_1891) comYC 1647912..1648238 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  H1X04_RS08520 (STHERMO_1892) comYB 1648235..1649335 (-) 1101 WP_148148900.1 competence type IV pilus assembly protein ComGB Machinery gene
  H1X04_RS08525 (STHERMO_1893) comGA/cglA/cilD 1649217..1650158 (-) 942 WP_011226629.1 competence type IV pilus ATPase ComGA Machinery gene
  H1X04_RS08530 (STHERMO_1894) - 1650239..1650601 (-) 363 WP_011226630.1 DUF1033 family protein -
  H1X04_RS08535 (STHERMO_1895) rpoC 1650760..1654398 (-) 3639 WP_011226631.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35422.56 Da        Isoelectric Point: 6.3927

>NTDB_id=1132209 H1X04_RS08525 WP_011226629.1 1649217..1650158(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_2101]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMNYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1132209 H1X04_RS08525 WP_011226629.1 1649217..1650158(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_2101]
ATGGTAACAGAATTTGCTAAGGAAATGATAAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTTTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCTATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GAACTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGTGTAACTGTTCTCTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCTACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.273

99.361

0.649
  comYA Streptococcus mutans UA159

64.537

100

0.645
  comYA Streptococcus mutans UA140

64.537

100

0.645
  comGA/cglA/cilD Streptococcus pneumoniae D39

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae R6

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

63.987

99.361

0.636
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.218

99.681

0.61
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

38.816

97.125

0.377

Multiple sequence alignment