Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   H1W91_RS08915 Genome accession   NZ_LR822032
Coordinates   1710285..1711226 (-) Length   313 a.a.
NCBI ID   WP_022096896.1    Uniprot ID   -
Organism   Streptococcus thermophilus isolate STH_CIRM_1050     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1705285..1716226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W91_RS08875 (STHERMO_1966) - 1705361..1706554 (-) 1194 WP_014608740.1 acetate kinase -
  H1W91_RS08880 (STHERMO_1967) comYH 1706610..1707566 (-) 957 WP_180477178.1 class I SAM-dependent methyltransferase Machinery gene
  H1W91_RS08885 (STHERMO_1968) comGG 1707611..1707928 (-) 318 WP_180477179.1 competence type IV pilus minor pilin ComGG -
  H1W91_RS08890 (STHERMO_1969) comYF 1707906..1708343 (-) 438 WP_082309153.1 competence type IV pilus minor pilin ComGF Machinery gene
  H1W91_RS08895 (STHERMO_1970) comGE 1708327..1708620 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  H1W91_RS08900 (STHERMO_1971) comGD 1708592..1709020 (-) 429 WP_253269448.1 competence type IV pilus minor pilin ComGD -
  H1W91_RS08905 (STHERMO_1972) comYC 1708980..1709306 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  H1W91_RS08910 (STHERMO_1973) comYB 1709303..1710403 (-) 1101 WP_120764773.1 competence type IV pilus assembly protein ComGB Machinery gene
  H1W91_RS08915 (STHERMO_1974) comGA/cglA/cilD 1710285..1711226 (-) 942 WP_022096896.1 competence type IV pilus ATPase ComGA Machinery gene
  H1W91_RS08920 (STHERMO_1975) - 1711307..1711669 (-) 363 WP_014608746.1 DUF1033 family protein -
  H1W91_RS08925 (STHERMO_1976) rpoC 1711828..1715466 (-) 3639 WP_011226631.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35409.56 Da        Isoelectric Point: 6.3927

>NTDB_id=1131610 H1W91_RS08915 WP_022096896.1 1710285..1711226(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_1050]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1131610 H1W91_RS08915 WP_022096896.1 1710285..1711226(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_1050]
ATGGTAACAGAATTTGCTAAGGAAATGATAAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTTTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCTGAACGTCGGGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCGATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGGGTAACTGTTCTTTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.595

99.361

0.652
  comYA Streptococcus mutans UA159

64.856

100

0.649
  comYA Streptococcus mutans UA140

64.856

100

0.649
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.309

99.361

0.639
  comYA Streptococcus gordonii str. Challis substr. CH1

63.259

100

0.633
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.538

99.681

0.613
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

38.816

97.125

0.377

Multiple sequence alignment