Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   H0514_RS00830 Genome accession   NZ_LR822020
Coordinates   147182..148123 (+) Length   313 a.a.
NCBI ID   WP_179972347.1    Uniprot ID   A0A7U7H347
Organism   Streptococcus thermophilus isolate STH_CIRM_956     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 142182..153123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0514_RS00820 (STHERMO_0165) rpoC 142942..146580 (+) 3639 WP_179973253.1 DNA-directed RNA polymerase subunit beta' -
  H0514_RS00825 (STHERMO_0166) - 146739..147101 (+) 363 WP_179972348.1 DUF1033 family protein -
  H0514_RS00830 (STHERMO_0167) comGA/cglA/cilD 147182..148123 (+) 942 WP_179972347.1 competence type IV pilus ATPase ComGA Machinery gene
  H0514_RS00835 (STHERMO_0168) comYB 148005..149105 (+) 1101 WP_179973355.1 competence type IV pilus assembly protein ComGB Machinery gene
  H0514_RS00840 (STHERMO_0169) comYC 149102..149428 (+) 327 WP_179972346.1 competence type IV pilus major pilin ComGC Machinery gene
  H0514_RS00845 (STHERMO_0170) comGD 149388..149816 (+) 429 WP_269473123.1 competence type IV pilus minor pilin ComGD -
  H0514_RS00850 (STHERMO_0171) comGE 149788..150081 (+) 294 WP_179972344.1 competence type IV pilus minor pilin ComGE -
  H0514_RS00855 (STHERMO_0172) comYF 150065..150502 (+) 438 WP_179972343.1 competence type IV pilus minor pilin ComGF Machinery gene
  H0514_RS00860 (STHERMO_0173) comGG 150480..150791 (+) 312 WP_179972342.1 competence type IV pilus minor pilin ComGG -
  H0514_RS00865 (STHERMO_0174) comYH 150842..151798 (+) 957 WP_179972341.1 class I SAM-dependent methyltransferase Machinery gene
  H0514_RS00870 (STHERMO_0175) - 151854..153047 (+) 1194 WP_179972340.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35597.82 Da        Isoelectric Point: 6.1042

>NTDB_id=1131064 H0514_RS00830 WP_179972347.1 147182..148123(+) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_956]
MVTEFAKEMIKNADSCGAQDIYFIPRQDKYELYMRVGQERRLIDVYRPDFMASLISHFKFVAGMMVGEKRRSQLGSCDYD
CDDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIMDALDYRGLYLFAGPVGSGKTTLMHELVQECFKGQ
QVISIEDPVEIKQDDVLQLQVNKAIDMTYDSLIKLSLRHRPDILIIGEIRDKETARAVIRASLTGVTVLSTIHAKSITGV
YERLLDFGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNHQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1131064 H0514_RS00830 WP_179972347.1 147182..148123(+) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_956]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATTTCATTCCACGTCA
GGATAAATATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAAAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGATGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTATGGATGCCTTGGATTATCGAGGAC
TGTATCTTTTTGCGGGTCCCGTAGGTTCTGGGAAGACTACACTTATGCATGAGTTGGTTCAGGAGTGTTTTAAGGGGCAG
CAGGTAATTTCGATTGAGGATCCTGTTGAAATCAAACAGGATGATGTCTTGCAACTCCAGGTTAATAAGGCAATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATATTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATCAGAGCTAGTCTAACAGGTGTAACTGTTCTTTCAACTATTCATGCTAAGAGTATTACTGGCGTT
TACGAACGTCTCTTGGACTTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCATCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7H347

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.595

99.361

0.652
  comYA Streptococcus mutans UA159

64.856

100

0.649
  comYA Streptococcus mutans UA140

64.856

100

0.649
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.952

99.361

0.645
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.952

99.361

0.645
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.952

99.361

0.645
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.952

99.361

0.645
  comYA Streptococcus gordonii str. Challis substr. CH1

63.898

100

0.639
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.897

99.681

0.607
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

38.591

95.208

0.367

Multiple sequence alignment