Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   H0514_RS00835 Genome accession   NZ_LR822020
Coordinates   148005..149105 (+) Length   366 a.a.
NCBI ID   WP_179973355.1    Uniprot ID   -
Organism   Streptococcus thermophilus isolate STH_CIRM_956     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 143005..154105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0514_RS00825 (STHERMO_0166) - 146739..147101 (+) 363 WP_179972348.1 DUF1033 family protein -
  H0514_RS00830 (STHERMO_0167) comGA/cglA/cilD 147182..148123 (+) 942 WP_179972347.1 competence type IV pilus ATPase ComGA Machinery gene
  H0514_RS00835 (STHERMO_0168) comYB 148005..149105 (+) 1101 WP_179973355.1 competence type IV pilus assembly protein ComGB Machinery gene
  H0514_RS00840 (STHERMO_0169) comYC 149102..149428 (+) 327 WP_179972346.1 competence type IV pilus major pilin ComGC Machinery gene
  H0514_RS00845 (STHERMO_0170) comGD 149388..149816 (+) 429 WP_269473123.1 competence type IV pilus minor pilin ComGD -
  H0514_RS00850 (STHERMO_0171) comGE 149788..150081 (+) 294 WP_179972344.1 competence type IV pilus minor pilin ComGE -
  H0514_RS00855 (STHERMO_0172) comYF 150065..150502 (+) 438 WP_179972343.1 competence type IV pilus minor pilin ComGF Machinery gene
  H0514_RS00860 (STHERMO_0173) comGG 150480..150791 (+) 312 WP_179972342.1 competence type IV pilus minor pilin ComGG -
  H0514_RS00865 (STHERMO_0174) comYH 150842..151798 (+) 957 WP_179972341.1 class I SAM-dependent methyltransferase Machinery gene
  H0514_RS00870 (STHERMO_0175) - 151854..153047 (+) 1194 WP_179972340.1 acetate kinase -
  H0514_RS00875 (STHERMO_0176) - 153295..153492 (+) 198 WP_179972339.1 helix-turn-helix transcriptional regulator -
  H0514_RS11190 (STHERMO_0177) - 153504..153945 (+) 442 Protein_129 CAAX protease -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 42075.24 Da        Isoelectric Point: 10.1295

>NTDB_id=1131065 H0514_RS00835 WP_179973355.1 148005..149105(+) (comYB) [Streptococcus thermophilus isolate STH_CIRM_956]
MPEKISKTIRRPTGIISWKVWLNKDVSLRGISKGKKLKISQQVKVIQLFKQLLKAGFTLTEIVAFLERSHLLKETSLSLM
KESLIRGDRLDQMFALVGFSDNIVTQISLADKHGNLLGSLTKIETYMLRMTKVRKKLMEVATYPILLLGFLILIMLGLKN
YLLPQLLEGDGRNNWAVQLVQIFPQLFFVSLCRFLVLGLILYLWVKRQSALVFYRRMAQIPFIGQIVRLYTTAYYAREWG
NLLGQGVDLLDLVALMQEQKSKLFRELGADLEEALMLGQSFPERIASHPFFTKELSLIIAYGEANARLGYELEVYAEEVW
QNFFNRLNKATTFVQPLIFVIIAVVIVMIYVAMLLPMYQNMEGMMS

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=1131065 H0514_RS00835 WP_179973355.1 148005..149105(+) (comYB) [Streptococcus thermophilus isolate STH_CIRM_956]
TTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCATCAGTTGGAAGGTTTGGTTAAACAAGGATGTCTC
ACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAGTCAGCAAGTTAAGGTTATCCAGCTCTTCAAACAACTTCTAA
AGGCCGGTTTTACCTTAACTGAAATCGTAGCCTTTTTGGAGCGAAGTCACTTGCTGAAAGAAACGTCCTTGTCTCTTATG
AAGGAGAGTTTAATACGCGGTGATAGGTTGGATCAGATGTTCGCGTTAGTGGGGTTTTCGGACAATATTGTTACTCAGAT
TTCTCTTGCTGACAAGCACGGTAATCTTCTAGGGAGTCTAACAAAGATTGAAACCTATATGCTTCGTATGACAAAGGTTC
GCAAGAAACTCATGGAGGTGGCGACTTATCCCATACTACTTCTAGGTTTTCTGATTCTGATTATGCTAGGACTTAAAAAT
TATCTTCTACCCCAACTCTTAGAGGGTGATGGTAGGAATAATTGGGCTGTACAGTTGGTTCAAATTTTTCCTCAGCTCTT
TTTTGTGAGTTTGTGTAGATTCCTTGTGTTGGGCTTAATTCTCTATCTATGGGTGAAACGACAGTCAGCCCTTGTTTTTT
ATAGGCGAATGGCCCAAATCCCTTTTATTGGTCAGATAGTAAGGCTTTACACGACCGCCTATTATGCTAGGGAATGGGGA
AATTTATTAGGTCAAGGCGTTGATTTGCTAGATTTGGTGGCTCTTATGCAAGAGCAAAAGTCTAAACTCTTCCGTGAGCT
GGGAGCCGATTTGGAAGAAGCCCTGATGCTGGGACAGAGTTTTCCTGAACGTATTGCCAGTCATCCGTTTTTTACTAAGG
AGCTCTCGCTAATTATTGCTTATGGAGAGGCCAATGCGAGGTTGGGCTATGAGTTGGAAGTTTATGCCGAGGAGGTTTGG
CAAAACTTCTTTAACCGTCTTAATAAGGCAACAACCTTTGTGCAACCCCTCATTTTTGTTATTATTGCAGTTGTGATTGT
GATGATCTATGTAGCCATGCTATTACCAATGTATCAAAATATGGAAGGAATGATGTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(236-356)

Type II secretion system (T2SS), protein F (T2SSF)

(51-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

54.678

93.443

0.511
  comYB Streptococcus mutans UA159

54.678

93.443

0.511
  comYB Streptococcus gordonii str. Challis substr. CH1

50.585

93.443

0.473
  comGB/cglB Streptococcus mitis NCTC 12261

50.151

90.437

0.454
  comGB/cglB Streptococcus mitis SK321

49.394

90.164

0.445
  comGB/cglB Streptococcus pneumoniae Rx1

49.39

89.617

0.443
  comGB/cglB Streptococcus pneumoniae D39

49.39

89.617

0.443
  comGB/cglB Streptococcus pneumoniae R6

49.39

89.617

0.443
  comGB/cglB Streptococcus pneumoniae TIGR4

49.39

89.617

0.443
  comGB Lactococcus lactis subsp. cremoris KW2

43.769

89.891

0.393

Multiple sequence alignment