Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   FGK99_RS00710 Genome accession   NZ_LR594033
Coordinates   124245..125183 (+) Length   312 a.a.
NCBI ID   WP_021320259.1    Uniprot ID   -
Organism   Streptococcus equi subsp. zooepidemicus strain NCTC4675     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 119245..130183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGK99_RS00695 (NCTC4675_00140) - 119611..120843 (+) 1233 WP_014622136.1 hypothetical protein -
  FGK99_RS00700 (NCTC4675_00141) - 121416..123419 (+) 2004 WP_043024947.1 DUF1430 domain-containing protein -
  FGK99_RS00705 (NCTC4675_00142) - 123421..124089 (+) 669 WP_043024949.1 ATP-binding cassette domain-containing protein -
  FGK99_RS00710 (NCTC4675_00143) comGA/cglA 124245..125183 (+) 939 WP_021320259.1 competence type IV pilus ATPase ComGA Machinery gene
  FGK99_RS00715 (NCTC4675_00144) comYB 125116..126150 (+) 1035 WP_229296759.1 competence type IV pilus assembly protein ComGB Machinery gene
  FGK99_RS00720 (NCTC4675_00145) comGC/cglC 126151..126474 (+) 324 WP_012514782.1 competence type IV pilus major pilin ComGC Machinery gene
  FGK99_RS00725 (NCTC4675_00146) comGD 126452..126877 (+) 426 WP_012514783.1 competence type IV pilus minor pilin ComGD -
  FGK99_RS00730 (NCTC4675_00147) comGE 126882..127124 (+) 243 WP_012677236.1 competence type IV pilus minor pilin ComGE -
  FGK99_RS00735 (NCTC4675_00148) comYF 127111..127545 (+) 435 WP_012677237.1 competence type IV pilus minor pilin ComGF Machinery gene
  FGK99_RS00740 (NCTC4675_00149) comGG 127523..127885 (+) 363 WP_043024957.1 competence type IV pilus minor pilin ComGG -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35341.58 Da        Isoelectric Point: 7.1375

>NTDB_id=1127155 FGK99_RS00710 WP_021320259.1 124245..125183(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain NCTC4675]
MVQELAKRLIRQAEELQAQDIYILPRGASYELLMRIGDDKRLIDVCESDRMANLISHFKFVAGMNVGEKRRCQLGACDYD
LDGTKVSLRLSAVGDYQGQESLVIRLLYHHQRQLRYWFDGLERVTSAIGARGLYLFSGPVGSGKTTLMYQLVSDYCKELQ
VISIEDPVEIKNDQLLQLQVNDSIGMTYDNLIKLSLRHRPDVLIIGEIRDTQTARAVIRASLTGAMVFSTVHAKSISGVY
ARLLELGISRAELDNSLAMVTYQRFISGGALIDCAQKEFEHHQANRWNQQIDQLLAEGHLNTRQARLEKIIQ

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1127155 FGK99_RS00710 WP_021320259.1 124245..125183(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain NCTC4675]
ATGGTTCAAGAATTAGCCAAGAGGCTTATCAGGCAAGCCGAAGAATTGCAGGCTCAGGATATTTATATACTGCCAAGAGG
GGCAAGCTATGAGCTTTTGATGAGGATAGGTGATGACAAGAGGCTAATAGATGTTTGTGAAAGTGATCGGATGGCTAATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGAATGTGGGAGAAAAAAGACGCTGCCAGCTCGGTGCTTGTGACTACGAC
CTTGATGGCACTAAGGTATCTCTACGATTATCAGCTGTGGGTGATTATCAGGGACAGGAGAGCTTGGTTATTCGTCTCTT
GTATCATCATCAACGCCAGCTCAGGTATTGGTTTGATGGTTTAGAGCGAGTCACATCAGCTATTGGAGCTAGAGGCCTTT
ACCTCTTTTCTGGACCAGTCGGATCAGGTAAGACAACCCTGATGTATCAGCTAGTCTCTGACTATTGCAAGGAGCTTCAG
GTGATCAGTATTGAGGATCCTGTGGAAATAAAAAATGACCAGCTCCTGCAATTACAGGTTAACGACAGCATTGGCATGAC
ATACGATAATCTGATTAAGCTCTCCTTGCGTCATCGTCCAGATGTGTTGATTATCGGAGAAATCAGAGATACACAGACTG
CTAGAGCGGTTATTAGGGCTAGCCTAACAGGAGCAATGGTCTTTTCAACCGTCCATGCAAAGAGTATTTCAGGCGTTTAC
GCTAGGCTGCTAGAATTAGGCATTTCTAGGGCAGAATTGGATAATAGTCTAGCAATGGTTACTTACCAGCGATTTATTAG
TGGAGGTGCTTTAATTGATTGTGCACAAAAGGAATTTGAACATCATCAAGCCAACCGGTGGAATCAGCAGATTGATCAGC
TTCTTGCAGAGGGACATCTCAATACCAGGCAGGCAAGGCTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

68.065

99.359

0.676
  comYA Streptococcus mutans UA140

63.344

99.679

0.631
  comYA Streptococcus mutans UA159

63.344

99.679

0.631
  comYA Streptococcus gordonii str. Challis substr. CH1

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae D39

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae R6

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

60.968

99.359

0.606
  comGA Lactococcus lactis subsp. cremoris KW2

52.733

99.679

0.526
  comGA Latilactobacillus sakei subsp. sakei 23K

39.583

92.308

0.365

Multiple sequence alignment