Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ALT761_RS15690 Genome accession   NZ_LR136958
Coordinates   3641298..3642410 (-) Length   370 a.a.
NCBI ID   WP_129739897.1    Uniprot ID   -
Organism   Alteromonas sp. 76-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3636298..3647410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALT761_RS20675 (ALT761_03198) - 3637470..3637634 (+) 165 WP_013782911.1 hypothetical protein -
  ALT761_RS15665 (ALT761_03199) - 3637782..3638510 (+) 729 WP_129739892.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ALT761_RS15670 (ALT761_03200) gshB 3638723..3639676 (+) 954 WP_129739893.1 glutathione synthase -
  ALT761_RS15675 (ALT761_03201) - 3639719..3640285 (+) 567 WP_129739894.1 YqgE/AlgH family protein -
  ALT761_RS15680 (ALT761_03202) ruvX 3640278..3640712 (+) 435 WP_129739895.1 Holliday junction resolvase RuvX -
  ALT761_RS15685 (ALT761_03203) - 3640978..3641292 (-) 315 WP_129739896.1 DUF2007 domain-containing protein -
  ALT761_RS15690 (ALT761_03204) pilU 3641298..3642410 (-) 1113 WP_129739897.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ALT761_RS15695 (ALT761_03205) pilT 3642420..3643463 (-) 1044 WP_057789891.1 type IV pilus twitching motility protein PilT Machinery gene
  ALT761_RS15700 (ALT761_03206) - 3643488..3644174 (+) 687 WP_129739898.1 YggS family pyridoxal phosphate-dependent enzyme -
  ALT761_RS15705 (ALT761_03207) proC 3644231..3645052 (+) 822 WP_129739899.1 pyrroline-5-carboxylate reductase -
  ALT761_RS15710 (ALT761_03208) - 3645074..3645613 (+) 540 WP_013782901.1 YggT family protein -
  ALT761_RS15715 (ALT761_03209) - 3645645..3646076 (+) 432 WP_232043598.1 DUF4426 domain-containing protein -
  ALT761_RS15720 (ALT761_03210) - 3646303..3647241 (+) 939 WP_232043475.1 transposase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40916.61 Da        Isoelectric Point: 5.2756

>NTDB_id=1125815 ALT761_RS15690 WP_129739897.1 3641298..3642410(-) (pilU) [Alteromonas sp. 76-1]
MLDSFLEKMVEKSASDLFVTAGFPVSAKVHGKLTPISENSFNDEDALELVHEAMNEKQKEAFHTTKECNFAIVREGLGRF
RCSAFWQRDQAGMVVRRIVTEIPKVDDLGLPSVLKDIIMSKRGLVLFVGGTGTGKSTSLAALIGHRNSNSYGHILTIEDP
IEFVHEHKSCVITQREVGIDTHSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSQNIKAIVAQQLLPTKDGEGRVAAIEILLNSPLVSDLIQRNEIGSLKEAMKKGKELGMQSFDMAL
YDLYREGSIDLDQALHHADSPNDLRLMIKLDSNDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1125815 ALT761_RS15690 WP_129739897.1 3641298..3642410(-) (pilU) [Alteromonas sp. 76-1]
ATGTTAGATAGCTTTTTAGAAAAAATGGTAGAGAAATCTGCTTCTGACTTATTCGTTACCGCGGGCTTTCCCGTTAGCGC
CAAAGTGCACGGTAAGCTTACTCCCATTTCGGAAAATTCATTTAACGATGAAGATGCATTAGAACTTGTCCATGAGGCTA
TGAATGAAAAGCAAAAAGAGGCTTTTCATACTACCAAGGAATGTAATTTTGCCATTGTACGAGAAGGCCTAGGGCGTTTT
CGTTGTTCGGCGTTCTGGCAACGAGATCAAGCCGGGATGGTGGTACGTCGTATTGTGACCGAAATTCCCAAGGTCGATGA
CTTGGGTTTACCTTCGGTGTTAAAAGATATCATTATGTCAAAGCGCGGCTTGGTGCTTTTTGTAGGTGGCACAGGCACGG
GTAAATCGACCTCGCTGGCCGCACTTATTGGTCATCGAAATAGTAATTCTTATGGGCATATTTTAACTATTGAAGATCCT
ATTGAATTTGTGCATGAGCATAAATCTTGCGTGATAACTCAGCGTGAAGTGGGTATAGACACCCACTCATTCGACGATGC
ATTGAAAAGCTCCTTGCGACAAGCGCCTGATGTCATTCTTATTGGTGAAATTCGTTCAATGGAGACAATGGAATACGCCA
TGTCGTTCGCTGATACGGGGCATTTATGTGTGGCAACACTCCATGCCAACAACGCAAACCAAGCAATTGAGCGCATTATG
CATTTAGCGCCAAAAGACCAACATGACAAGTTACGTTTTGACTTAAGCCAAAATATAAAAGCTATAGTGGCACAACAACT
TCTTCCAACCAAAGACGGCGAGGGAAGGGTAGCGGCAATTGAAATACTATTAAACTCTCCTCTAGTGAGCGACCTTATTC
AACGCAATGAAATAGGTAGCTTGAAAGAAGCCATGAAAAAGGGCAAGGAGTTAGGCATGCAAAGTTTCGATATGGCGTTG
TACGATTTATATCGCGAGGGCAGTATTGATTTAGACCAAGCCTTACACCATGCTGATTCTCCCAATGATTTGCGCCTAAT
GATTAAGCTAGATTCTAACGATGGATCGAGCTTGGGCACATTATCGAATGTCTCAATCGATATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.569

100

0.597
  pilU Acinetobacter baylyi ADP1

56.164

98.649

0.554
  pilU Vibrio cholerae strain A1552

55.435

99.459

0.551
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.21

95.405

0.403
  pilT Acinetobacter baumannii D1279779

41.566

89.73

0.373
  pilT Acinetobacter nosocomialis M2

41.566

89.73

0.373
  pilT Acinetobacter baumannii strain A118

41.566

89.73

0.373
  pilT Legionella pneumophila strain Lp02

42.188

86.486

0.365
  pilT Legionella pneumophila strain ERS1305867

42.188

86.486

0.365
  pilT Acinetobacter baylyi ADP1

40.663

89.73

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.12

90.27

0.362

Multiple sequence alignment