Detailed information NTG1122955

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EL223_RS12145
• Genome accession: NZ_LR134474
• Coordinates: 2488790..2489239 (-)
• Length: 149 a.a.
• NCBI ID: WP_126344133.1
• Uniprot ID: -
• Organism: Kurthia zopfii strain NCTC404
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2483790..2494239

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL223_RS12120 (NCTC404_02545)	-	2484100..2484399 (-)	300	WP_126344125.1	DUF3139 domain-containing protein	-
EL223_RS12125 (NCTC404_02546)	holA	2484516..2485529 (-)	1014	WP_126344127.1	DNA polymerase III subunit delta	-
EL223_RS12130 (NCTC404_02547)	-	2485680..2485814 (+)	135	WP_126344129.1	YqzM family protein	-
EL223_RS12135 (NCTC404_02548)	-	2485886..2487964 (-)	2079	WP_172597358.1	DNA internalization-related competence protein ComEC/Rec2	-
EL223_RS12140 (NCTC404_02549)	-	2488144..2488725 (-)	582	WP_109348344.1	ComE operon protein 2	-
EL223_RS12145 (NCTC404_02550)	comEA	2488790..2489239 (-)	450	WP_126344133.1	helix-hairpin-helix domain-containing protein	Machinery gene
EL223_RS12150 (NCTC404_02551)	-	2489477..2490685 (-)	1209	WP_126344624.1	ISL3 family transposase	-
EL223_RS12155 (NCTC404_02552)	-	2491069..2491806 (-)	738	WP_126344134.1	class I SAM-dependent DNA methyltransferase	-
EL223_RS12160 (NCTC404_02553)	rsfS	2491799..2492155 (-)	357	WP_126344136.1	ribosome silencing factor	-
EL223_RS12165 (NCTC404_02554)	yqeK	2492159..2492728 (-)	570	WP_126344138.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EL223_RS12170 (NCTC404_02555)	-	2492715..2493287 (-)	573	WP_126344140.1	nicotinate-nucleotide adenylyltransferase	-
EL223_RS12175 (NCTC404_02556)	yhbY	2493302..2493607 (-)	306	WP_109348338.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 15902.87 Da
• Isoelectric Point: 4.8895

>NTDB_id=1122955 EL223_RS12145 WP_126344133.1 2488790..2489239(-) (comEA) [Kurthia zopfii strain NCTC404]
MDVKGAVKKPGIYELDPNARVMDAISLAGGYISTADRNGINLAAKLQDEMVIYVPKKGENKDNPIIQSPASSPTGQQNSS
GSEAQSVVNINTADETQLQTLSGIGPAKAQAIISYRTDTGLFQTIEDLKKVTGFGEKTFERLQSSITVQ

Nucleotide

• Length: 450 bp

>NTDB_id=1122955 EL223_RS12145 WP_126344133.1 2488790..2489239(-) (comEA) [Kurthia zopfii strain NCTC404]
GTGGATGTTAAAGGTGCTGTTAAAAAACCCGGTATTTACGAACTGGATCCAAATGCGAGAGTAATGGATGCAATCTCATT
AGCTGGTGGTTATATTTCGACGGCTGATCGAAATGGTATTAATTTGGCTGCAAAACTTCAAGATGAGATGGTCATTTATG
TCCCTAAAAAGGGGGAGAATAAAGACAATCCTATTATTCAAAGTCCAGCATCTTCGCCTACAGGACAACAAAATTCTTCT
GGAAGTGAAGCGCAGTCTGTAGTGAATATTAATACGGCAGATGAGACTCAATTGCAGACGCTTAGTGGAATAGGACCTGC
TAAAGCACAAGCGATTATCTCCTATCGCACCGATACGGGGTTATTTCAGACGATTGAAGATCTCAAAAAAGTGACAGGAT
TCGGAGAAAAAACTTTCGAAAGATTACAAAGTTCTATAACAGTTCAGTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (85-147)

• SLBB domain (SLBB) (3-54)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Latilactobacillus sakei subsp. sakei 23K	46.914	100	0.51
comEA	Bacillus subtilis subsp. subtilis str. 168	48.387	100	0.503
comEA	Lactococcus lactis subsp. cremoris KW2	49.324	99.329	0.49
comEA	Streptococcus thermophilus LMD-9	45.695	100	0.463
comEA/celA/cilE	Streptococcus pneumoniae D39	42.568	99.329	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	42.568	99.329	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	42.568	99.329	0.423
comEA/celA/cilE	Streptococcus mitis SK321	42.568	99.329	0.423
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	42.568	99.329	0.423
comEA/celA/cilE	Streptococcus pneumoniae Rx1	42.568	99.329	0.423
comEA	Staphylococcus aureus N315	41.216	99.329	0.409
comEA	Staphylococcus aureus MW2	41.216	99.329	0.409