Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EL064_RS05870 Genome accession   NZ_LR134178
Coordinates   1359690..1360121 (-) Length   143 a.a.
NCBI ID   WP_115705063.1    Uniprot ID   -
Organism   Legionella sainthelensi strain NCTC12450     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1354690..1365121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL064_RS05845 (NCTC12450_01237) - 1354773..1356728 (+) 1956 WP_115705057.1 ATP-dependent DNA helicase -
  EL064_RS05850 (NCTC12450_01238) tsaB 1356709..1357359 (+) 651 WP_115705059.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  EL064_RS05855 (NCTC12450_01239) - 1357369..1357548 (+) 180 WP_027270259.1 Trm112 family protein -
  EL064_RS05860 (NCTC12450_01240) - 1357714..1359114 (-) 1401 WP_027270258.1 APC family permease -
  EL064_RS05865 (NCTC12450_01241) - 1359280..1359603 (-) 324 WP_115705061.1 BolA/IbaG family iron-sulfur metabolism protein -
  EL064_RS05870 (NCTC12450_01242) pilA 1359690..1360121 (-) 432 WP_115705063.1 pilin Machinery gene
  EL064_RS05875 (NCTC12450_01243) - 1360409..1361653 (-) 1245 WP_128129675.1 6-phosphofructokinase -
  EL064_RS05880 (NCTC12450_01245) - 1361694..1364428 (-) 2735 Protein_1174 ATP-binding protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14751.95 Da        Isoelectric Point: 6.2100

>NTDB_id=1118808 EL064_RS05870 WP_115705063.1 1359690..1360121(-) (pilA) [Legionella sainthelensi strain NCTC12450]
MKQKGFTLIELMIVVAIIGILAAIAIPAYQDYVVRARVTEGLSLASAAKTTVSENASSGAAKLSQGWVAPTATANVKSIT
VDDNTGVITITYDTPAKSIVITLTPQSGGAALVAGTPPTDAITWTCAVDQAANDRYVPSNCRI

Nucleotide


Download         Length: 432 bp        

>NTDB_id=1118808 EL064_RS05870 WP_115705063.1 1359690..1360121(-) (pilA) [Legionella sainthelensi strain NCTC12450]
ATGAAGCAAAAGGGTTTTACTTTAATTGAATTGATGATAGTAGTGGCAATCATTGGAATTCTCGCCGCAATCGCTATTCC
TGCATATCAAGACTATGTCGTTAGAGCTCGTGTTACGGAAGGATTAAGCCTTGCCTCTGCAGCAAAAACGACAGTTTCTG
AGAACGCATCAAGTGGTGCTGCGAAATTAAGCCAAGGCTGGGTTGCCCCTACAGCAACTGCTAATGTAAAAAGCATTACT
GTAGATGATAATACGGGAGTAATCACCATCACTTATGATACGCCGGCAAAAAGTATTGTGATTACTTTAACCCCACAATC
TGGTGGTGCAGCTCTTGTTGCTGGTACTCCGCCAACAGATGCTATTACCTGGACTTGTGCGGTTGATCAGGCTGCAAACG
ACCGATATGTTCCGTCCAATTGCCGTATTTAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(34-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

50.955

100

0.559
  pilA2 Legionella pneumophila strain ERS1305867

53.378

100

0.552
  pilA2 Legionella pneumophila str. Paris

53.378

100

0.552
  comP Acinetobacter baylyi ADP1

49.032

100

0.531
  pilA/pilA1 Eikenella corrodens VA1

39.744

100

0.434
  pilA Acinetobacter baumannii strain A118

41.216

100

0.427
  pilA Pseudomonas aeruginosa PAK

38.065

100

0.413
  pilE Neisseria gonorrhoeae MS11

36.025

100

0.406
  pilE Neisseria gonorrhoeae strain FA1090

35.625

100

0.399
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.762

100

0.378
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.324

99.301

0.371

Multiple sequence alignment