Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   A66_RS09250 Genome accession   NZ_LN847353
Coordinates   1757565..1758506 (-) Length   313 a.a.
NCBI ID   WP_000249571.1    Uniprot ID   A0A0B7M3Q7
Organism   Streptococcus pneumoniae strain A66     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1752565..1763506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A66_RS09210 - 1752911..1753514 (+) 604 Protein_1790 transposase family protein -
  A66_RS09215 (A66_01840) - 1753665..1754141 (+) 477 WP_000777686.1 GNAT family N-acetyltransferase -
  A66_RS09220 (A66_01841) comGG/cglG 1754796..1755209 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  A66_RS09225 (A66_01842) comGF/cglF 1755187..1755648 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  A66_RS09230 (A66_01843) comGE/cglE 1755611..1755913 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  A66_RS09235 (A66_01844) comGD/cglD 1755876..1756280 (-) 405 WP_050365326.1 competence type IV pilus minor pilin ComGD Machinery gene
  A66_RS09240 (A66_01845) comGC/cglC 1756273..1756599 (-) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  A66_RS09245 (A66_01846) comGB/cglB 1756601..1757617 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  A66_RS09250 (A66_01847) comGA/cglA/cilD 1757565..1758506 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  A66_RS09255 (A66_01848) - 1758582..1758947 (-) 366 WP_000286415.1 DUF1033 family protein -
  A66_RS09260 (A66_01849) - 1759098..1760156 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  A66_RS09265 (A66_01850) nagA 1760319..1761470 (-) 1152 WP_001134454.1 N-acetylglucosamine-6-phosphate deacetylase -
  A66_RS09270 (A66_01851) - 1761623..1763440 (-) 1818 WP_053039773.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35591.51 Da        Isoelectric Point: 6.0083

>NTDB_id=1114605 A66_RS09250 WP_000249571.1 1757565..1758506(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain A66]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1114605 A66_RS09250 WP_000249571.1 1757565..1758506(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain A66]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M3Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361

Multiple sequence alignment