Detailed information NTG1114600

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: A66_RS09225
• Genome accession: NZ_LN847353
• Coordinates: 1755187..1755648 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250545.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain A66
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1750187..1760648

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A66_RS12810 (A66_01835)	-	1750306..1750437 (-)	132	WP_000768904.1	hypothetical protein	-
A66_RS09200 (A66_01836)	-	1750438..1751628 (-)	1191	WP_053039772.1	acetate kinase	-
A66_RS09205 (A66_01837)	comYH	1751679..1752632 (-)	954	WP_000345126.1	class I SAM-dependent methyltransferase	Machinery gene
A66_RS09210	-	1752911..1753514 (+)	604	Protein_1790	transposase family protein	-
A66_RS09215 (A66_01840)	-	1753665..1754141 (+)	477	WP_000777686.1	GNAT family N-acetyltransferase	-
A66_RS09220 (A66_01841)	comGG/cglG	1754796..1755209 (-)	414	WP_000265630.1	competence type IV pilus minor pilin ComGG	Machinery gene
A66_RS09225 (A66_01842)	comGF/cglF	1755187..1755648 (-)	462	WP_000250545.1	competence type IV pilus minor pilin ComGF	Machinery gene
A66_RS09230 (A66_01843)	comGE/cglE	1755611..1755913 (-)	303	WP_000413380.1	competence type IV pilus minor pilin ComGE	Machinery gene
A66_RS09235 (A66_01844)	comGD/cglD	1755876..1756280 (-)	405	WP_050365326.1	competence type IV pilus minor pilin ComGD	Machinery gene
A66_RS09240 (A66_01845)	comGC/cglC	1756273..1756599 (-)	327	WP_000738630.1	comG operon protein ComGC	Machinery gene
A66_RS09245 (A66_01846)	comGB/cglB	1756601..1757617 (-)	1017	WP_074017570.1	competence type IV pilus assembly protein ComGB	Machinery gene
A66_RS09250 (A66_01847)	comGA/cglA/cilD	1757565..1758506 (-)	942	WP_000249571.1	competence type IV pilus ATPase ComGA	Machinery gene
A66_RS09255 (A66_01848)	-	1758582..1758947 (-)	366	WP_000286415.1	DUF1033 family protein	-
A66_RS09260 (A66_01849)	-	1759098..1760156 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17855.34 Da
• Isoelectric Point: 6.4832

>NTDB_id=1114600 A66_RS09225 WP_000250545.1 1755187..1755648(-) (comGF/cglF) [Streptococcus pneumoniae strain A66]
MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RFYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=1114600 A66_RS09225 WP_000250545.1 1755187..1755648(-) (comGF/cglF) [Streptococcus pneumoniae strain A66]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGCT
TGTCATCAGTGGAGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTGGAAGGCAAT
CGTTTCTATATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae D39	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae R6	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae TIGR4	93.464	100	0.935
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915