Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   A66_RS09245 Genome accession   NZ_LN847353
Coordinates   1756601..1757617 (-) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain A66     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1751601..1762617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A66_RS09205 (A66_01837) comYH 1751679..1752632 (-) 954 WP_000345126.1 class I SAM-dependent methyltransferase Machinery gene
  A66_RS09210 - 1752911..1753514 (+) 604 Protein_1790 transposase family protein -
  A66_RS09215 (A66_01840) - 1753665..1754141 (+) 477 WP_000777686.1 GNAT family N-acetyltransferase -
  A66_RS09220 (A66_01841) comGG/cglG 1754796..1755209 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  A66_RS09225 (A66_01842) comGF/cglF 1755187..1755648 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  A66_RS09230 (A66_01843) comGE/cglE 1755611..1755913 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  A66_RS09235 (A66_01844) comGD/cglD 1755876..1756280 (-) 405 WP_050365326.1 competence type IV pilus minor pilin ComGD Machinery gene
  A66_RS09240 (A66_01845) comGC/cglC 1756273..1756599 (-) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  A66_RS09245 (A66_01846) comGB/cglB 1756601..1757617 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  A66_RS09250 (A66_01847) comGA/cglA/cilD 1757565..1758506 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  A66_RS09255 (A66_01848) - 1758582..1758947 (-) 366 WP_000286415.1 DUF1033 family protein -
  A66_RS09260 (A66_01849) - 1759098..1760156 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  A66_RS09265 (A66_01850) nagA 1760319..1761470 (-) 1152 WP_001134454.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=1114604 A66_RS09245 WP_074017570.1 1756601..1757617(-) (comGB/cglB) [Streptococcus pneumoniae strain A66]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1114604 A66_RS09245 WP_074017570.1 1756601..1757617(-) (comGB/cglB) [Streptococcus pneumoniae strain A66]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment