Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KHN94_RS13315 Genome accession   NZ_HG992758
Coordinates   2923207..2924940 (-) Length   577 a.a.
NCBI ID   WP_182011506.1    Uniprot ID   -
Organism   Vibrio sp. B1FLJ16 isolate B1REV17     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2918207..2929940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHN94_RS13295 (ACOMICROBIO_LOCUS1900) - 2918836..2919675 (-) 840 WP_182032325.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  KHN94_RS13300 (ACOMICROBIO_LOCUS1901) - 2919745..2921235 (-) 1491 WP_182032324.1 SPOR domain-containing protein -
  KHN94_RS13305 (ACOMICROBIO_LOCUS1902) aroB 2921368..2922468 (-) 1101 WP_182032323.1 3-dehydroquinate synthase -
  KHN94_RS13310 (ACOMICROBIO_LOCUS1903) aroK 2922500..2923018 (-) 519 WP_182011505.1 shikimate kinase AroK -
  KHN94_RS13315 (ACOMICROBIO_LOCUS1904) pilQ 2923207..2924940 (-) 1734 WP_182011506.1 type IV pilus secretin PilQ family protein Machinery gene
  KHN94_RS13320 (ACOMICROBIO_LOCUS1905) pilP 2924972..2925487 (-) 516 WP_182011507.1 pilus assembly protein PilP Machinery gene
  KHN94_RS13325 (ACOMICROBIO_LOCUS1906) pilO 2925477..2926070 (-) 594 WP_182011508.1 type 4a pilus biogenesis protein PilO Machinery gene
  KHN94_RS13330 (ACOMICROBIO_LOCUS1907) pilN 2926063..2926638 (-) 576 WP_182032322.1 PilN domain-containing protein Machinery gene
  KHN94_RS13335 (ACOMICROBIO_LOCUS1908) pilM 2926622..2927638 (-) 1017 WP_182032321.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63283.18 Da        Isoelectric Point: 4.4706

>NTDB_id=1112539 KHN94_RS13315 WP_182011506.1 2923207..2924940(-) (pilQ) [Vibrio sp. B1FLJ16 isolate B1REV17]
MKMRQGLTRTPRMLTGLLSFWLFLLLAPLVAAEDVTSNALKSIDFRTNKDKDAVIVVELSSPAAIVDIQRVQEGLSIDLL
NTSVRDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHIGDDYTHDYRLTGRYLEIKVSKLKPNQKVPDQSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTNELDLREKQQ
LEKQQLEEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSSRGSMTIDERTNSLLIRELPENISVIRDIIESLDIPVK
QVQIEARIVTISEGNMDELGIRWGFSSINGNNTVGGSIENNLATIGLYEGDGEGGNSVGIDDFLNVNLASTSPSATSIAF
QVAKLGTDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQITPDN
RLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRRSYEN
VGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1112539 KHN94_RS13315 WP_182011506.1 2923207..2924940(-) (pilQ) [Vibrio sp. B1FLJ16 isolate B1REV17]
ATGAAAATGAGACAAGGACTAACACGTACACCTAGGATGCTAACCGGGCTCTTATCCTTTTGGTTGTTTTTACTTTTAGC
CCCTTTGGTTGCGGCAGAAGATGTGACATCTAATGCTCTCAAGAGTATCGACTTTCGCACCAATAAAGACAAAGACGCAG
TGATAGTGGTGGAACTCTCTTCACCCGCAGCGATTGTTGATATTCAGCGTGTACAGGAAGGCTTAAGTATCGACTTACTG
AATACATCAGTGAGAGACGATCAATTATACCTTCTTGATGTAAAGGACTTTTCTACCGTGGTGGAGAGTGTTGAAGTTTT
TCGTGATACCTCAACGACGCGTTTAGTGGCCCATATCGGTGATGACTATACCCATGATTACCGCCTGACCGGGCGCTATC
TGGAAATCAAAGTAAGTAAACTGAAGCCAAACCAGAAGGTACCGGATCAGAGCATACTGGAGAAAGAGGGGAAGCTGATC
TCGATTAACTTCCAGGATATCCCTGTGCGTAACGTACTGCAGTTAATCGCTGACTATAACCAGTTTAACCTGGTGGTTTC
AGATTCAGTTGAAGGCAACTTAACTCTGCGACTCGATGGCGTTCCCTGGCAGCAAGTGCTGGATATTATCCTGCAGGTAA
AAGGCCTCGATAAGCGTGTTGACGGTAATGTTATCCTTGTCGCGCCGACAAATGAGCTGGACTTGCGTGAAAAGCAACAG
TTAGAGAAGCAACAGCTTGAAGAAGAGATGGGTGAGCTTTCGTCTGAAATTATCAAAGTTAACTTTGCTAAAGCGTCTGA
TATTGCTGAAATGATTAATGGTGAAGGTAATATAAGTATGCTCTCCTCACGTGGCAGCATGACGATTGATGAACGTACAA
ACTCGCTGTTGATCAGAGAACTGCCAGAAAACATTTCGGTGATTCGTGACATTATCGAGTCACTCGATATTCCGGTTAAG
CAAGTTCAAATCGAAGCTCGAATTGTCACCATCAGTGAAGGCAATATGGATGAGTTGGGTATTCGCTGGGGATTCTCTTC
TATCAACGGTAATAATACGGTTGGTGGTTCAATTGAAAATAACTTAGCAACCATAGGTTTGTATGAAGGGGATGGTGAAG
GCGGAAACAGTGTCGGAATTGATGACTTCCTTAACGTGAACCTCGCGTCGACCAGCCCGAGTGCAACCAGTATTGCTTTT
CAGGTTGCAAAACTAGGTACAGATACCTTGCTCGACCTTGAGCTGTCTGCACTCCAGCAGGAGTCGAAAGCGGAGATAAT
CTCTAGTCCTCGTTTGATTACGACGAATAAAAAGCCGGCCTACATCGAGCAGGGTACTGAAATACCATATTTAGAGTCAT
CATCGAGCGGTGCGACATCGGTCACATTCAAGAAAGCAGTATTAAGTCTGAAGGTGACGCCACAGATTACCCCTGATAAC
CGTCTGGTGTTGGATTTAAGTGTTACCCAGGACAGACCTGGACAAGTGGTGAAAACGGGTACCGGTGAAGCCGTTGCGAT
AGATACTCAGAGAATCGGTACTCAGGTATTGGTTAACAATGGTGAGACAGTTGTATTGGGCGGTATTTTCCAGCACAGCA
TCACTAACTCAGTCGACAAGGTGCCATTACTAGGTGATCTGCCGCTATTAGGTGCACTTTTCCGCAGAAGTTATGAGAAT
GTGGGCAAAAGTGAACTGTTGATTTTCGTTACACCGAAGGTTGTAATTCAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(417-575)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

91.379

100

0.919
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.554

97.574

0.747
  pilQ Vibrio cholerae strain A1552

76.554

97.574

0.747
  pilQ Legionella pneumophila strain ERS1305867

41.575

94.627

0.393
  pilQ Legionella pneumophila strain Lp02

41.575

94.627

0.393
  pilQ Pseudomonas aeruginosa PAK

40.217

95.667

0.385
  pilQ Acinetobacter baumannii D1279779

36.869

100

0.38
  pilQ Acinetobacter baumannii strain A118

36.869

100

0.38
  comQ Acinetobacter baylyi ADP1

37.674

99.827

0.376

Multiple sequence alignment