Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   BN5_RS23840 Genome accession   NZ_HG916826
Coordinates   718521..718955 (+) Length   144 a.a.
NCBI ID   WP_004424994.1    Uniprot ID   -
Organism   Ectopseudomonas oleovorans CECT 5344 strain CECT5344     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 713521..723955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN5_RS03225 (BN5_0661) coaE 713600..714208 (-) 609 WP_004425004.1 dephospho-CoA kinase -
  BN5_RS03230 (BN5_0662) pilD 714501..715370 (-) 870 WP_004425002.1 A24 family peptidase Machinery gene
  BN5_RS03235 (BN5_0663) pilC 715371..716588 (-) 1218 WP_004425000.1 type II secretion system F family protein Machinery gene
  BN5_RS03240 (BN5_0664) pilB 716591..718294 (-) 1704 WP_004424998.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BN5_RS23840 (BN5_0665) pilA/pilAI 718521..718955 (+) 435 WP_004424994.1 pilin Machinery gene
  BN5_RS03250 (BN5_0666) - 719750..720280 (+) 531 WP_004424990.1 transposase -
  BN5_RS03255 (BN5_0667) - 720350..720694 (-) 345 WP_004424987.1 type II toxin-antitoxin system HicB family antitoxin -
  BN5_RS22325 - 720691..720945 (-) 255 WP_074857836.1 type II toxin-antitoxin system HicA family toxin -
  BN5_RS03260 (BN5_0668) - 721163..721561 (+) 399 WP_004424982.1 nucleotidyltransferase domain-containing protein -
  BN5_RS03265 (BN5_0669) - 721551..721973 (+) 423 WP_039965073.1 DUF86 domain-containing protein -
  BN5_RS03270 (BN5_0670) - 722227..722616 (-) 390 WP_004424977.1 type II toxin-antitoxin system VapC family toxin -
  BN5_RS03275 (BN5_0671) - 722613..722870 (-) 258 WP_004424974.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  BN5_RS03280 (BN5_0672) - 723370..723729 (-) 360 Protein_664 nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14770.09 Da        Isoelectric Point: 8.0963

>NTDB_id=1112135 BN5_RS23840 WP_004424994.1 718521..718955(+) (pilA/pilAI) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
MKAQMQKGFTLIELMIVVAIIGILAAIAIPAYQDYVAKSKATAALADITGGKTQYELLATEGTTQTTTLVGLATETGNCS
TISVTATAAAGSPTTAALKCVIKNPGRLGADAAIQYDRTAGGLYECKTIKFADKKFAPAGCSDT

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1112135 BN5_RS23840 WP_004424994.1 718521..718955(+) (pilA/pilAI) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
ATGAAAGCTCAGATGCAAAAAGGTTTTACGCTGATTGAATTGATGATCGTTGTGGCGATCATTGGTATTTTGGCGGCAAT
TGCGATTCCGGCTTATCAAGATTACGTAGCCAAGTCGAAGGCAACGGCAGCGTTAGCCGATATCACTGGCGGCAAAACCC
AGTATGAGCTGCTAGCTACTGAAGGAACGACTCAGACCACTACTCTCGTGGGGCTGGCCACCGAAACTGGCAATTGCTCA
ACAATTTCCGTAACAGCGACCGCTGCAGCGGGCTCACCAACAACCGCAGCGCTAAAATGTGTAATCAAAAATCCAGGTCG
CCTGGGAGCTGACGCAGCCATTCAGTATGACCGTACGGCTGGAGGTCTGTATGAGTGCAAAACCATCAAATTTGCTGATA
AAAAATTCGCACCAGCAGGTTGCTCCGATACTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-28)

Pilin (bacterial filament) (Pilin)

(37-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.651

100

0.493
  pilA Pseudomonas aeruginosa PAK

41.615

100

0.465
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.06

100

0.431
  pilA Vibrio cholerae strain A1552

41.06

100

0.431
  pilA Vibrio cholerae C6706

41.06

100

0.431
  pilA Acinetobacter baumannii strain A118

41.549

98.611

0.41
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.845

98.611

0.403
  comP Acinetobacter baylyi ADP1

35.862

100

0.361

Multiple sequence alignment