Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BN5_RS03235 Genome accession   NZ_HG916826
Coordinates   715371..716588 (-) Length   405 a.a.
NCBI ID   WP_004425000.1    Uniprot ID   W6QS04
Organism   Ectopseudomonas oleovorans CECT 5344 strain CECT5344     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 710371..721588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN5_RS03195 (BN5_0655) - 710664..710837 (-) 174 WP_004425016.1 DUF3094 family protein -
  BN5_RS03200 (BN5_0656) - 710862..711410 (-) 549 WP_004425015.1 MOSC domain-containing protein -
  BN5_RS03205 (BN5_0657) - 711407..712036 (-) 630 WP_004425012.1 DUF1780 domain-containing protein -
  BN5_RS03210 (BN5_0658) - 712234..712923 (+) 690 WP_004425010.1 energy-coupling factor ABC transporter permease -
  BN5_RS03215 (BN5_0659) - 713030..713245 (+) 216 WP_004425008.1 hypothetical protein -
  BN5_RS03220 (BN5_0660) yacG 713409..713603 (-) 195 WP_004425006.1 DNA gyrase inhibitor YacG -
  BN5_RS03225 (BN5_0661) coaE 713600..714208 (-) 609 WP_004425004.1 dephospho-CoA kinase -
  BN5_RS03230 (BN5_0662) pilD 714501..715370 (-) 870 WP_004425002.1 A24 family peptidase Machinery gene
  BN5_RS03235 (BN5_0663) pilC 715371..716588 (-) 1218 WP_004425000.1 type II secretion system F family protein Machinery gene
  BN5_RS03240 (BN5_0664) pilB 716591..718294 (-) 1704 WP_004424998.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BN5_RS23840 (BN5_0665) pilA/pilAI 718521..718955 (+) 435 WP_004424994.1 pilin Machinery gene
  BN5_RS03250 (BN5_0666) - 719750..720280 (+) 531 WP_004424990.1 transposase -
  BN5_RS03255 (BN5_0667) - 720350..720694 (-) 345 WP_004424987.1 type II toxin-antitoxin system HicB family antitoxin -
  BN5_RS22325 - 720691..720945 (-) 255 WP_074857836.1 type II toxin-antitoxin system HicA family toxin -
  BN5_RS03260 (BN5_0668) - 721163..721561 (+) 399 WP_004424982.1 nucleotidyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43963.74 Da        Isoelectric Point: 9.8564

>NTDB_id=1112109 BN5_RS03235 WP_004425000.1 715371..716588(-) (pilC) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
MAGKALKTSVFTWEGKNKSGAVVKGELSGQNPSLVKAQLRKQGINPTKVRKKGVSLFSAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIISEGFDNPNMRKLVDEVKQEVAAGNSFAASLRKKPQYFDELYCNLVESGEQAGALENLLDRIATYKEKT
EALKAKIKKAMNYPIAVIVVAIIVSAILLIKVVPQFEEVFANFGAELPAFTLMVIGISQFLQEWWFIFLIAIIAAAFAFK
EAMKRSQKLRDSVDRGVLKLPIVGDILYKSAVARFARTLSTTFAAGVPLVDALDSVSGATGNVVFKNATNKIKADVSTGM
QLNFSMRTTGTFPSMAVQMTAIGEESGSLDEMLDKVASFYEAEVDNMVDGLTSLMEPIIMSVLGVLIGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1112109 BN5_RS03235 WP_004425000.1 715371..716588(-) (pilC) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
ATGGCGGGAAAAGCTCTAAAAACCAGTGTGTTCACCTGGGAAGGCAAGAACAAAAGCGGTGCCGTCGTCAAGGGGGAGCT
CAGCGGGCAGAACCCGTCCTTGGTCAAGGCGCAACTGCGCAAACAGGGGATCAATCCGACCAAAGTACGCAAAAAGGGGG
TGTCGCTCTTCAGCGCAGGCAAGAAGATCAAACCCATGGATATCGCCCTGTTTACCCGGCAGATGGCGACCATGATGAAG
GCTGGGGTCCCGCTGCTGCAGTCTTTCGACATCATTTCCGAAGGTTTCGACAACCCCAACATGCGCAAGCTGGTCGACGA
GGTGAAGCAGGAGGTGGCTGCTGGTAACAGTTTTGCCGCCTCGCTGCGCAAGAAACCACAGTACTTCGATGAACTGTATT
GCAACCTGGTTGAGTCTGGCGAACAGGCCGGCGCCCTGGAAAACCTGCTGGACCGAATCGCCACCTACAAGGAAAAGACC
GAGGCGCTCAAAGCCAAGATCAAAAAAGCGATGAATTACCCTATCGCTGTTATCGTGGTCGCCATCATCGTCTCAGCCAT
TTTGCTGATTAAAGTGGTGCCACAGTTCGAAGAGGTATTTGCTAACTTCGGCGCTGAACTACCTGCCTTTACGCTGATGG
TAATAGGCATATCGCAATTTCTTCAGGAGTGGTGGTTCATTTTTCTGATTGCAATCATCGCCGCTGCCTTTGCATTCAAA
GAAGCGATGAAGCGCTCGCAGAAACTGCGCGACTCGGTTGACCGTGGCGTACTGAAACTGCCAATCGTCGGCGACATTCT
TTACAAATCTGCGGTAGCCCGCTTCGCACGCACACTATCAACGACTTTTGCCGCTGGCGTGCCACTGGTCGATGCGCTGG
ATTCGGTCTCCGGCGCCACCGGTAATGTGGTGTTCAAGAATGCGACCAACAAGATCAAGGCCGATGTCTCGACTGGCATG
CAGCTTAACTTCTCAATGCGCACCACCGGCACCTTCCCCTCCATGGCTGTGCAGATGACCGCTATTGGCGAAGAGTCTGG
CTCGCTGGATGAGATGCTCGATAAGGTAGCGAGCTTCTATGAAGCAGAAGTGGACAACATGGTTGACGGCCTGACCAGCC
TGATGGAACCCATCATCATGTCCGTACTGGGCGTACTGATCGGCGGTCTGATCATCGCCATGTACCTACCTATCTTCCAG
TTGGGTGCCGTCGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W6QS04

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.222

100

0.822
  pilC Acinetobacter baumannii D1279779

59.901

99.753

0.598
  pilC Acinetobacter baylyi ADP1

60.804

98.272

0.598
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilG Neisseria gonorrhoeae MS11

46.02

99.259

0.457
  pilG Neisseria meningitidis 44/76-A

46.02

99.259

0.457
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Vibrio campbellii strain DS40M4

40.152

97.778

0.393

Multiple sequence alignment