Detailed information NTG1112108

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BN5_RS03230
• Genome accession: NZ_HG916826
• Coordinates: 714501..715370 (-)
• Length: 289 a.a.
• NCBI ID: WP_004425002.1
• Uniprot ID: W6QYS6
• Organism: Ectopseudomonas oleovorans CECT 5344 strain CECT5344
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 709501..720370

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BN5_RS03195 (BN5_0655)	-	710664..710837 (-)	174	WP_004425016.1	DUF3094 family protein	-
BN5_RS03200 (BN5_0656)	-	710862..711410 (-)	549	WP_004425015.1	MOSC domain-containing protein	-
BN5_RS03205 (BN5_0657)	-	711407..712036 (-)	630	WP_004425012.1	DUF1780 domain-containing protein	-
BN5_RS03210 (BN5_0658)	-	712234..712923 (+)	690	WP_004425010.1	energy-coupling factor ABC transporter permease	-
BN5_RS03215 (BN5_0659)	-	713030..713245 (+)	216	WP_004425008.1	hypothetical protein	-
BN5_RS03220 (BN5_0660)	yacG	713409..713603 (-)	195	WP_004425006.1	DNA gyrase inhibitor YacG	-
BN5_RS03225 (BN5_0661)	coaE	713600..714208 (-)	609	WP_004425004.1	dephospho-CoA kinase	-
BN5_RS03230 (BN5_0662)	pilD	714501..715370 (-)	870	WP_004425002.1	A24 family peptidase	Machinery gene
BN5_RS03235 (BN5_0663)	pilC	715371..716588 (-)	1218	WP_004425000.1	type II secretion system F family protein	Machinery gene
BN5_RS03240 (BN5_0664)	pilB	716591..718294 (-)	1704	WP_004424998.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BN5_RS23840 (BN5_0665)	pilA/pilAI	718521..718955 (+)	435	WP_004424994.1	pilin	Machinery gene
BN5_RS03250 (BN5_0666)	-	719750..720280 (+)	531	WP_004424990.1	transposase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31822.95 Da
• Isoelectric Point: 6.8190

>NTDB_id=1112108 BN5_RS03230 WP_004425002.1 714501..715370(-) (pilD) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
MLVIDFLASHALAFVLCALLLGLLIGSFLNVVIYRLPLMLQRDWQSQAREVLELPSAPTAQTFNLILPNSCCPQCGHEIR
PWENIPVISYLFLRGKCSNCKTPISKRYPIVELACGLLSGYIAWHFGFTWQAGAMLVLAWGLLAMSLIDADHQILPDVLV
LPLLWLGLIVNYFGLFTSLEDALWGAIAGYLSLWSVYWLFKLITGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVILLRMRGSDSGTPIPFGPYLAIAGWIALLWGDVITGSYLKFAGF

Nucleotide

• Length: 870 bp

>NTDB_id=1112108 BN5_RS03230 WP_004425002.1 714501..715370(-) (pilD) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
ATGCTCGTGATCGACTTTCTGGCCAGCCATGCGCTGGCCTTTGTTTTGTGTGCTCTGCTACTGGGCCTGCTGATTGGCAG
CTTCCTCAATGTAGTGATCTATCGCCTACCGTTGATGCTGCAACGTGATTGGCAGAGTCAGGCGCGCGAGGTGCTGGAAT
TGCCGTCGGCACCAACCGCCCAGACCTTCAACCTGATCCTGCCCAATTCCTGCTGCCCGCAATGCGGCCATGAGATTCGC
CCCTGGGAGAACATCCCGGTCATCAGCTATCTGTTTCTGCGCGGCAAGTGTTCGAACTGCAAGACTCCGATCAGCAAGCG
TTACCCCATCGTTGAACTGGCCTGCGGGCTGCTTTCCGGTTACATCGCCTGGCATTTCGGTTTCACCTGGCAGGCCGGGG
CGATGCTGGTGCTGGCCTGGGGGCTGCTGGCGATGAGCCTGATCGATGCTGATCACCAGATTCTGCCCGACGTGCTGGTG
CTGCCTCTGCTATGGCTGGGATTGATCGTCAATTACTTCGGACTGTTCACCAGCCTGGAAGATGCTCTGTGGGGGGCGAT
TGCCGGTTATCTGAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCTGATCACCGGCAAGGAAGGCATGGGTTACGGTGACT
TCAAGCTGCTGGCGATGCTCGGGGCCTGGGGTGGCTGGCAGATTCTGCCGTTGACCATCCTGCTGTCTTCGCTGGTCGGT
GCGGTGCTAGGTGTGATTCTGCTGCGCATGCGTGGCAGCGATTCCGGCACGCCGATTCCCTTCGGCCCCTATCTGGCGAT
TGCCGGGTGGATCGCCCTGCTGTGGGGTGATGTGATTACCGGCAGTTATCTGAAGTTCGCTGGGTTTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

3D structure

• AlphaFold DB: W6QYS6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.38	94.81	0.516
pilD	Acinetobacter baumannii D1279779	52.113	98.27	0.512
pilD	Acinetobacter nosocomialis M2	51.761	98.27	0.509
pilD	Vibrio campbellii strain DS40M4	53.285	94.81	0.505
pilD	Neisseria gonorrhoeae MS11	50.19	91.003	0.457