Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BN5_RS03240 Genome accession   NZ_HG916826
Coordinates   716591..718294 (-) Length   567 a.a.
NCBI ID   WP_004424998.1    Uniprot ID   -
Organism   Ectopseudomonas oleovorans CECT 5344 strain CECT5344     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 711591..723294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN5_RS03210 (BN5_0658) - 712234..712923 (+) 690 WP_004425010.1 energy-coupling factor ABC transporter permease -
  BN5_RS03215 (BN5_0659) - 713030..713245 (+) 216 WP_004425008.1 hypothetical protein -
  BN5_RS03220 (BN5_0660) yacG 713409..713603 (-) 195 WP_004425006.1 DNA gyrase inhibitor YacG -
  BN5_RS03225 (BN5_0661) coaE 713600..714208 (-) 609 WP_004425004.1 dephospho-CoA kinase -
  BN5_RS03230 (BN5_0662) pilD 714501..715370 (-) 870 WP_004425002.1 A24 family peptidase Machinery gene
  BN5_RS03235 (BN5_0663) pilC 715371..716588 (-) 1218 WP_004425000.1 type II secretion system F family protein Machinery gene
  BN5_RS03240 (BN5_0664) pilB 716591..718294 (-) 1704 WP_004424998.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BN5_RS23840 (BN5_0665) pilA/pilAI 718521..718955 (+) 435 WP_004424994.1 pilin Machinery gene
  BN5_RS03250 (BN5_0666) - 719750..720280 (+) 531 WP_004424990.1 transposase -
  BN5_RS03255 (BN5_0667) - 720350..720694 (-) 345 WP_004424987.1 type II toxin-antitoxin system HicB family antitoxin -
  BN5_RS22325 - 720691..720945 (-) 255 WP_074857836.1 type II toxin-antitoxin system HicA family toxin -
  BN5_RS03260 (BN5_0668) - 721163..721561 (+) 399 WP_004424982.1 nucleotidyltransferase domain-containing protein -
  BN5_RS03265 (BN5_0669) - 721551..721973 (+) 423 WP_039965073.1 DUF86 domain-containing protein -
  BN5_RS03270 (BN5_0670) - 722227..722616 (-) 390 WP_004424977.1 type II toxin-antitoxin system VapC family toxin -
  BN5_RS03275 (BN5_0671) - 722613..722870 (-) 258 WP_004424974.1 type II toxin-antitoxin system Phd/YefM family antitoxin -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62062.07 Da        Isoelectric Point: 5.3527

>NTDB_id=1112110 BN5_RS03240 WP_004424998.1 716591..718294(-) (pilB) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
MTDNIALSGLAKQMVLTGLLDDKTAQQAQQQAARNKLSLVTYVVQNKLAKGRAVAELASDQFGVALLDLSALDKESLPKE
LVSEKLARQHRVLPLSRRGTKLYVAISDPANHQAITDIQFSTGLSIESILVEDDKLGEAIDKLYESGTTGLEELGEVDLD
GVDVESVTEDGKAEEGGEAADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRFRTDGILHEVARPPIQLAPRISAR
LKVMAALDISERRKPQDGRIKMRVSKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYMSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIRD
LDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNSCKKEVDVPRETLLAEGFP
EDKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPNLQRIIMEEGNSIDIAAQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1112110 BN5_RS03240 WP_004424998.1 716591..718294(-) (pilB) [Ectopseudomonas oleovorans CECT 5344 strain CECT5344]
ATGACGGACAATATCGCTCTATCCGGTCTCGCCAAGCAGATGGTGCTGACAGGTCTCCTTGACGACAAGACGGCGCAGCA
AGCGCAACAGCAGGCAGCGCGTAACAAGTTGTCGTTGGTTACCTATGTCGTCCAGAACAAGTTGGCCAAAGGCCGTGCCG
TGGCGGAACTCGCTTCGGATCAGTTCGGCGTGGCATTGCTGGACCTCTCTGCGCTTGACAAGGAGTCGCTGCCTAAAGAG
TTAGTCAGCGAGAAGCTAGCGAGGCAGCATCGTGTCCTGCCGCTCTCCAGGCGCGGTACTAAGCTATATGTCGCTATTTC
GGACCCTGCCAATCATCAGGCGATCACCGACATACAGTTCAGCACCGGTCTGAGCATCGAGTCGATCCTGGTGGAAGACG
ACAAGCTGGGTGAGGCCATCGACAAGCTGTACGAAAGCGGCACCACCGGACTAGAGGAGCTGGGCGAAGTCGATCTGGAT
GGGGTCGATGTCGAGTCCGTCACCGAAGATGGAAAAGCTGAGGAAGGCGGAGAGGCTGCTGACGACGCCCCTGTAGTGCG
CTTCGTCAACAAGATGCTGCTTGATGCAATAAAAGGTGGTTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGACCTATC
GCGTGCGCTTTCGTACCGACGGCATCCTCCATGAGGTTGCTCGCCCGCCTATTCAGTTGGCGCCGCGGATTTCAGCACGC
CTCAAGGTGATGGCCGCACTGGATATCTCCGAACGGCGCAAGCCGCAGGATGGCCGGATCAAGATGCGCGTATCCAAGAC
CAAGGCCATCGACTTTCGCGTCAACACCCTGCCTACCCTATGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCCACCA
GCGCGCAAATGGGCATCGACGCACTTGGCTACGAGGAGGAACAGAAAGAGCTGTATATGTCTGCCCTCAAGCAACCTCAA
GGTATGATTCTGGTCACCGGCCCCACCGGCTCGGGCAAAACCGTATCGCTCTATACCGGCCTGAACATCCTCAATACGCC
TGATGTGAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAGGGGATCAACCAGGTCAACGTGAATCCCAAGC
AGGGCATGGACTTTACCCAGGCGCTACGCGCCTTCCTGCGCCAGGACCCGGATATCATCATGGTCGGTGAGATCCGCGAT
CTGGATACCGCCTCCATTGCCGTCAAGGCGGCGCAAACCGGGCATATGGTGATGTCGACCCTGCACACCAACAGCGCTGC
CGAAACCCTGACCCGCCTGCGCAATATGGGCGTACCCTCCTTCAACATAGCCACCTCGGTCAACCTGATCATTGCCCAAC
GCCTGGCGCGCAAGCTTTGCAACAGTTGCAAGAAGGAAGTGGACGTGCCCCGCGAGACCCTGCTGGCCGAAGGCTTCCCT
GAGGACAAGATCGGTACCTTCAAGATCTATGGCCCAGTCGGCTGCGACAATTGCAAGGGCGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCCAACCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGATATTGCCGCGC
AGATGCGTAAAGACGGCTTTAATGACCTACGCACCTCCGCTTTGCTCAAGGCCATGCAAGGGGTCACCAGCCTTGAGGAA
GTCAACCGCGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.043

99.471

0.587
  pilB Acinetobacter baylyi ADP1

59.147

99.295

0.587
  pilB Legionella pneumophila strain ERS1305867

54.912

100

0.552
  pilB Vibrio parahaemolyticus RIMD 2210633

49.023

99.295

0.487
  pilB Vibrio campbellii strain DS40M4

47.965

99.647

0.478
  pilB Vibrio cholerae strain A1552

52.047

90.476

0.471
  pilF Neisseria gonorrhoeae MS11

51.346

91.711

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.212

94.004

0.369
  pilF Thermus thermophilus HB27

36.738

98.413

0.362

Multiple sequence alignment