Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACLKM5_RS12750 Genome accession   NZ_CP183893
Coordinates   2657607..2658050 (-) Length   147 a.a.
NCBI ID   WP_411687467.1    Uniprot ID   -
Organism   Acinetobacter indicus strain D10_NDM_tet(X3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2640665..2657553 2657607..2658050 flank 54


Gene organization within MGE regions


Location: 2640665..2658050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM5_RS12675 comE 2640665..2641126 (-) 462 WP_286396274.1 type IV pilin protein Machinery gene
  ACLKM5_RS12680 - 2641136..2645137 (-) 4002 WP_411687474.1 pilus assembly protein PilY -
  ACLKM5_RS12685 - 2645156..2645917 (-) 762 WP_180002716.1 pilus assembly PilX family protein -
  ACLKM5_RS12690 - 2645914..2646927 (-) 1014 WP_411687473.1 PilW family protein -
  ACLKM5_RS12695 pilV 2646930..2647436 (-) 507 WP_411687472.1 type IV pilus modification protein PilV -
  ACLKM5_RS12700 - 2647436..2647924 (-) 489 WP_411687471.1 GspH/FimT family pseudopilin -
  ACLKM5_RS12705 ispH 2648088..2649038 (-) 951 WP_411687494.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACLKM5_RS12710 gmk 2649174..2649797 (+) 624 WP_045795273.1 guanylate kinase -
  ACLKM5_RS12715 rpoZ 2649874..2650155 (+) 282 WP_004809957.1 DNA-directed RNA polymerase subunit omega -
  ACLKM5_RS12720 - 2650378..2652477 (+) 2100 WP_160240914.1 RelA/SpoT family protein -
  ACLKM5_RS12725 - 2652549..2652932 (+) 384 WP_104513240.1 RidA family protein -
  ACLKM5_RS12730 - 2653087..2653281 (+) 195 WP_034597991.1 bacterioferritin-associated ferredoxin -
  ACLKM5_RS12735 bfr 2653508..2653972 (+) 465 WP_411687470.1 heteropolymeric bacterioferritin subunit Bfr -
  ACLKM5_RS12740 - 2654084..2655724 (-) 1641 WP_411687469.1 PglL family O-oligosaccharyltransferase -
  ACLKM5_RS12745 - 2655946..2657553 (-) 1608 WP_411687468.1 PglL family O-oligosaccharyltransferase -
  ACLKM5_RS12750 pilA 2657607..2658050 (-) 444 WP_411687467.1 pilin Machinery gene

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15478.78 Da        Isoelectric Point: 5.1717

>NTDB_id=1106945 ACLKM5_RS12750 WP_411687467.1 2657607..2658050(-) (pilA) [Acinetobacter indicus strain D10_NDM_tet(X3)]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYTAKSQVAAGLAEISPAKTNAEAKLAEGISDTITSAPNLGVQETTNRC
DIEVSVAKNGVSTIQCKLKGTSSIDTKVIQWTRTEDKPDGTIGAWTCKTDVATDFMPKTCKTGATEI

Nucleotide


Download         Length: 444 bp        

>NTDB_id=1106945 ACLKM5_RS12750 WP_411687467.1 2657607..2658050(-) (pilA) [Acinetobacter indicus strain D10_NDM_tet(X3)]
ATGAACGCTCAAAAAGGTTTTACCTTAATTGAATTAATGATCGTTGTAGCGATTATTGGTATTCTTGCTGCAATTGCGAT
TCCTGCGTACCAAAACTATACAGCTAAGTCGCAAGTAGCTGCTGGTTTAGCAGAAATTTCACCTGCAAAAACAAATGCTG
AAGCTAAACTAGCAGAAGGTATTTCTGATACAATTACTTCAGCACCAAATTTAGGTGTTCAAGAAACTACTAATCGTTGT
GATATTGAGGTAAGTGTAGCGAAAAATGGTGTATCAACTATTCAGTGTAAACTTAAAGGTACATCATCTATTGATACTAA
AGTAATTCAATGGACACGTACTGAAGATAAACCAGATGGAACAATTGGTGCATGGACTTGTAAAACAGATGTGGCTACAG
ACTTTATGCCAAAAACATGTAAAACAGGCGCTACAGAAATTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

51.701

100

0.517
  comP Acinetobacter baylyi ADP1

42.675

100

0.456
  pilA Pseudomonas aeruginosa PAK

42.675

100

0.456
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.39

95.918

0.435
  pilA Vibrio cholerae C6706

42.568

100

0.429
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.568

100

0.429
  pilA Vibrio cholerae strain A1552

42.568

100

0.429
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.029

93.878

0.395
  pilA Vibrio parahaemolyticus RIMD 2210633

45.6

85.034

0.388
  pilE Neisseria gonorrhoeae strain FA1090

35.22

100

0.381
  pilA2 Legionella pneumophila strain ERS1305867

36.913

100

0.374

Multiple sequence alignment